Bonducellin
Based on 1 Customer Validation
Bonducellin is an efflux pump inhibitor and an EthD bromide (HY-D0021) sensitivity modulator. Bonducellin inhibits angiogenesis, proliferation, migration, and colony formation by regulating MMP9 gene expression, inhibiting iNOS and TNF-α/IL-12 production, inducing apoptosis, and targeting METTL3. Bonducellin can be used in research on tuberculosis, polycystic ovary syndrome, and colorectal cancer.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit : 99.66%
- CAS. Nr.: 83162-84-9
- Formel: C17H14O4
- Molecular Weight:282.29
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Speicherung:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Biologische Aktivität
Beschreibung
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iNOS |
TNF-α |
IL-12 |
METTL3 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Peritoneal macrophage | IC50 |
30 μM
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Inhibition of nitric oxide production in LPS/IFN-γ-stimulated murine peritoneal macrophages by Griess assay after 24 hrs incubation.
Inhibition of nitric oxide production in LPS/IFN-γ-stimulated murine peritoneal macrophages by Griess assay after 24 hrs incubation.
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15893896 |
| Peritoneal macrophage | IC50 |
20 μM
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Inhibition of TNF-α production in LPS/IFN-γ-stimulated murine peritoneal macrophages by ELISA.
Inhibition of TNF-α production in LPS/IFN-γ-stimulated murine peritoneal macrophages by ELISA.
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15893896 |
| SW480 | IC50 |
43.5 μM
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Antiproliferative activity against human SW480 colorectal cancer cells assessed as inhibition of proliferation.
Antiproliferative activity against human SW480 colorectal cancer cells assessed as inhibition of proliferation.
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v1 |
| SW-620 | IC50 |
51.3 μM
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Antiproliferative activity against human SW620 colorectal cancer cells assessed as inhibition of proliferation.
Antiproliferative activity against human SW620 colorectal cancer cells assessed as inhibition of proliferation.
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v1 |
In Vitro
Bonducellin (2.5-40 μM; 24 h after pre-activation) reduces NO production by 20% at 40 μM in pre-activated mouse peritoneal macrophages[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:murine peritoneal macrophage
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Concentration:2.5; 5; 10; 20; 40 μM
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Incubation Time:24 h
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Result:Did not reduce murine peritoneal macrophage viability in the MTT assay at the tested concentrations.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:zebrafish embryos[2]
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Dosage:0.8 μg/mL (LC50 dose at 48 hpf)
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Administration:static aqueous exposure in E3 medium; from 2 hpf; sampled at 24 and 48 hpf
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Result:At 24 hpf, downregulated MMP9 expression by 0.05-fold compared with control.
At 48 hpf, upregulated MMP9 expression by 2.4-fold compared with control.
Expression was normalized to β-Actin.
- RT-PCR
- RNA extraction experimental
- Human pluripotent stem cell endothelial-cell differentiation
- Vascular/Branching Fractal Analysis
- Annexin V plus membrane-impermeant dye apoptosis staining
- Cell migration
- Colony Formation (Clonogenic) Assay
- Real Time qPCR (Q-PCR)
- Cell Viability Determination by MTT Colorimetric Assay
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Endothelial Tube Formation Assay
- Apoptosis Solutions
- Soft Agar Colony Formation Assay
- Endothelial Cell Migration/Angiogenic Sprouting Assay
Chemical Information
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CAS. Nr. 83162-84-9
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Appearance Solid
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Molecular Weight 282.29
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Formel C17H14O4
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Color Off-white to light yellow
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SMILES
O=C1/C(COC2=CC(O)=CC=C12)=C/C3=CC=C(OC)C=C3
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Structure Classification
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Initial Source
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
In Vitro:
DMSO : 100 mg/mL (354.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.5 mg/mL (8.86 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Reinheit & Dokumentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Roy SK, et al. 7-Hydroxy-(E)-3-phenylmethylene-chroman-4-one analogues as efflux pump inhibitors against Mycobacterium smegmatis mc² 155. European journal of medicinal chemistry. 2013 Aug;66:499-507. [Content Brief]
[4]. Rao YK, et al. Anti-inflammatory activities of flavonoids isolated from Caesalpinia pulcherrima. Journal of ethnopharmacology. 2005 Sep 14;100(3):249-53. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.5425 mL | 17.7123 mL | 35.4246 mL | 88.5614 mL |
| 5 mM | 0.7085 mL | 3.5425 mL | 7.0849 mL | 17.7123 mL | |
| 10 mM | 0.3542 mL | 1.7712 mL | 3.5425 mL | 8.8561 mL | |
| 15 mM | 0.2362 mL | 1.1808 mL | 2.3616 mL | 5.9041 mL | |
| 20 mM | 0.1771 mL | 0.8856 mL | 1.7712 mL | 4.4281 mL | |
| 25 mM | 0.1417 mL | 0.7085 mL | 1.4170 mL | 3.5425 mL | |
| 30 mM | 0.1181 mL | 0.5904 mL | 1.1808 mL | 2.9520 mL | |
| 40 mM | 0.0886 mL | 0.4428 mL | 0.8856 mL | 2.2140 mL | |
| 50 mM | 0.0708 mL | 0.3542 mL | 0.7085 mL | 1.7712 mL | |
| 60 mM | 0.0590 mL | 0.2952 mL | 0.5904 mL | 1.4760 mL | |
| 80 mM | 0.0443 mL | 0.2214 mL | 0.4428 mL | 1.1070 mL | |
| 100 mM | 0.0354 mL | 0.1771 mL | 0.3542 mL | 0.8856 mL |
Bonducellin Related Classifications
HY-N10069 Related Classifications
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Keywords
- Bonducellin
- 83162-84-9
- NO Synthase
- TNF Receptor
- Interleukin Related
- Apoptosis
- METTL3
- IL-12
- METTL3 RNA methyltransferase
- MMP9
- Mycobacterium smegmatis
- TNF-alpha
- angiogenesis
- apoptosis
- colorectal cancer
- efflux pump inhibitor
- ethidium bromide
- extracellular matrix remodeling
- iNOS
- murine peritoneal macrophages
- ovarian function
- polycystic ovary syndrome
- tuberculosis
- zebrafish embryos
- Inhibitor
- inhibitor
- inhibit