TFEB activator 2
Based on 1 Customer Validation
TFEB activator 2 is an orally active compound that can cross the blood-brain barrier. TFEB activator 2 can bind to the dopamine transporter (DAT). TFEB activator 2 promotes TFEB nuclear translocation and lysosome biogenesis by targeting the DAT-CDK9-TFEB pathway. TFEB activator 2 has neuroprotective activity and can be used in the research of Alzheimer's disease and other diseases.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza : 98.84%
- No. CAS: 3027414-04-3
- Fòrmula: C26H29FN2O3
- Peso molecular:436.52
-
Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Actividad biológica
-
Chemical Information
-
Solvente y solubilidad
- Protocolo
- Pureza y Documentación
- Referencias
-
Help & FAQs
Actividad biológica
Descripciòn
In Vitro
TFEB activator 2 (Compound 37) (10-30 μM; 3-6 h) promotes TFEB nuclear translocation in HeLa cells[1].
TFEB activator 2 (Compound 37) (10-30 μM; 6-24 h) promotes lysosome biogenesis and upregulates the expression of lysosomal and autophagic genes in HeLa cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:HeLa cells
-
Concentration:10 μM
-
Incubation Time:24 h
-
Result:Increased the lysosomal genes of LAMP1, HEXA, CTSD and CTSF.
Increased the autophagic genes of LC3B, OPTN, p62, WIPI1 and ATG2A.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:Five-month-old APP/PS1 mice[1]
-
Dosage:10 mg/kg
-
Administration:Intraperitoneal injection; once every two days; 30 days
-
Result:Reduced Aβ plaque burden by 69.8% in the hippocampus and 29.5% in the cortex.
Decreased Aβ42 levels by 25.4% and 36.3% in the hippocampus and cortex, respectively.
Improved spatial learning and memory, as shown by reduced escape latency in the Morris Water Maze and increased platform crossings, and enhanced working memory in the Y maze.
Upregulated the expression of lysosomal and autophagic genes (e.g., LAMP1, CTSB) in the brain.
Chemical Information
-
No. CAS 3027414-04-3
-
Appearance Solid
-
Peso molecular 436.52
-
Fòrmula C26H29FN2O3
-
Color White to off-white
-
SMILES
FC1=CC2=C(C=C1)NC=C2CCC3C4=C(CCN3CC5CCOCC5)C=C6OCOC6=C4
-
Envío
Room temperature in continental US; may vary elsewhere.
-
Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (229.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 5 mg/mL (11.45 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
-
-
-
-
Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
-
%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
-
Ficha de datos (279 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2908 mL | 11.4542 mL | 22.9085 mL | 57.2711 mL |
| 5 mM | 0.4582 mL | 2.2908 mL | 4.5817 mL | 11.4542 mL | |
| 10 mM | 0.2291 mL | 1.1454 mL | 2.2908 mL | 5.7271 mL | |
| 15 mM | 0.1527 mL | 0.7636 mL | 1.5272 mL | 3.8181 mL | |
| 20 mM | 0.1145 mL | 0.5727 mL | 1.1454 mL | 2.8636 mL | |
| 25 mM | 0.0916 mL | 0.4582 mL | 0.9163 mL | 2.2908 mL | |
| 30 mM | 0.0764 mL | 0.3818 mL | 0.7636 mL | 1.9090 mL | |
| 40 mM | 0.0573 mL | 0.2864 mL | 0.5727 mL | 1.4318 mL | |
| 50 mM | 0.0458 mL | 0.2291 mL | 0.4582 mL | 1.1454 mL | |
| 60 mM | 0.0382 mL | 0.1909 mL | 0.3818 mL | 0.9545 mL | |
| 80 mM | 0.0286 mL | 0.1432 mL | 0.2864 mL | 0.7159 mL | |
| 100 mM | 0.0229 mL | 0.1145 mL | 0.2291 mL | 0.5727 mL |
TFEB activator 2 Related Classifications
HY-161724 Related Classifications
Powered by Bioz