JRN73958
JRN73958 (Reduced scytonemin) is a PI3K/Akt, MAPK, and NF-κB inhibitor found in Nostoc commune. JRN73958 inhibits nitric oxide production, induce reactive oxygen species (ROS) generation, and lead to autophagy. JRN73958 decreases LPS (HY-D1056)/IFNγ-induced PI3K/Akt, MAPK, and NF-κB activity. JRN73958 can be used for the research of leukemia.
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- No. CAS: 171773-95-8
- Fòrmula: C36H24N2O4
- Peso molecular:548.59
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Descripciòn
In Vitro
JRN73958 (0.5-2.5 μM; 2 h pretreatment) suppresses LPS/IFNγ-induced NO production in murine macrophage RAW264 cells in a concentration-dependent manner, with the strongest inhibition observed at 2.5 μM[1].
JRN73958 (2.5 μM; 2 h pretreatment) significantly reduces LPS/IFNγ-induced iNOS and COX-2 mRNA and protein expression in murine macrophage RAW264 cells[1].
JRN73958 (2.5 μM; 2 h pretreatment) inhibits LPS/IFNγ-mediated activation of p38 MAPK, ERK, SAPK/JNK, IκB, and STAT1 in murine macrophage RAW264 cells[1].
JRN73958 (2.5 μM; 2 h pretreatment) increases HO-1 expression 3.5-fold in murine macrophage RAW264 cells, and this induction is required for JRN73958's inhibitory effect on LPS/IFNγ-induced NO production[1].
JRN73958 (2.5 μM; 15-120 min) transiently increases intracellular ROS levels in murine macrophage RAW264 cells, reaching a 1.8-fold peak at 30 min[1].
JRN73958 (2.5 μM; 24 h) activates Nrf2/ARE signaling in murine macrophage RAW264 cells, increasing ARE enhancer activity to 1.35-fold relative to untreated cells[1].
JRN73958 (2.5 μM; 2 h) has its induced HO-1 expression in murine macrophage RAW264 cells suppressed by pretreatment with the antioxidant NAC or the kinase inhibitors SB203580 and LY294002[1].
JRN73958 (2.5 μM; 5-120 min pretreatment) activates p38 MAPK and PI3K/Akt signaling in murine macrophage RAW264 cells, with peak phosphorylation observed at 30 min[1].
JRN73958 (1.0-5.0 μM; 24-72 h) inhibits the growth of human T-lymphoid Jurkat cells with an IC50 of 1.8 μM[2].
JRN73958 (2.5 μM; 3-12 h) induces nuclear swelling, nuclear fragmentation, and reduced mitochondrial membrane potential in human T-lymphoid Jurkat cells[2].
JRN73958 (2.5-5.0 μM; 6-24 h) induces only marginal nucleosomal DNA fragmentation in human T-lymphoid Jurkat cells[2].
JRN73958 (2.5 μM; 72 h) induces human T-lymphoid Jurkat cell death that is resistant to caspase-8, caspase-9, and pan-caspase inhibitors after 72 h of treatment[2].
JRN73958 (2.5 μM; 12 h) induces the formation of multiple vacuoles and autophagosomes in human T-lymphoid Jurkat cells after 12 h of treatment[2].
JRN73958 (2.5 μM; 6-24 h) induces the conversion of LC3-I to LC3-II, a marker of autophagy, in human T-lymphoid Jurkat cells after 6, 12, and 24 h of treatment[2].
JRN73958 (2.5 μM; 12 h) induces colocalization of LC3 and lysosomes, marking autophagosome/autolysosome formation, in human T-lymphoid Jurkat cells after 12 h of treatment[2].
JRN73958 (2.5 μM; 48 h) induces autophagic cell growth inhibition in human T-lymphoid Jurkat cells after 48 h of treatment, which is reversible by the autophagy inhibitor 3-MA[2].
JRN73958 (2.5 μM; 48 h) induces human T-lymphoid Jurkat cell growth inhibition after 48 h of treatment, which is reversible by the antioxidant NAC[2].
JRN73958 (2.5 μM; 0.5-24 h) inhibits Akt phosphorylation in a time-dependent manner in human T-lymphoid Jurkat cells over 0.5, 1, 3, 6, 12, and 24 h of treatment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:murine macrophage RAW264 cells
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Concentration:2.5 μM
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Incubation Time:2 h
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Result:Remarkably suppressed LPS/IFNγ-stimulated p38 MAPK and ERK activation.
Slightly suppressed SAPK/JNK and IκB activation up to 30 and 60 min respectively.
Strongly suppressed STAT1 activation.
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Cell Line:human T-lymphoid Jurkat cells
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Concentration:2.5 μM
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Incubation Time:6 h; 12 h; 24 h
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Result:Increased levels of both LC3-I and LC3-II in Jurkat cells up to 24 h after treatment, indicating conversion of LC3-I to LC3-II.
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Cell Line:human T-lymphoid Jurkat cells
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Concentration:2.5 μM
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Incubation Time:48 h
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Result:Induced autophagic cell growth inhibition that was significantly blocked by the autophagy inhibitor 3-MA.\nInduced Jurkat cell growth inhibition that was reversed by pretreatment with the antioxidant NAC.
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Cell Line:human T-lymphoid Jurkat cells
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Concentration:2.5 μM
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Incubation Time:0.5 h; 1 h; 3 h; 6 h; 12 h; 24 h
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Result:Inhibited phosphorylation of Akt in a time-dependent manner, with reduced p-Akt levels observed from 0.5 h onwards.
Chemical Information
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No. CAS 171773-95-8
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Peso molecular 548.59
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Fòrmula C36H24N2O4
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SMILES
O=C1/C(C2NC3=C(C2=C1C(C/4=O)=C5C6=C(C=CC=C6)NC5C4=C\C7=CC=C(C=C7)O)C=CC=C3)=C\C8=CC=C(C=C8)O
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Synonyms
Reduced scytonemin
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Structure Classification
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Initial Source
Lyngbya sp. CU2555
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. Itoh T, et al. Reduced scytonemin isolated from Nostoc commune suppresses LPS/IFNγ-induced NO production in murine macrophage RAW264 cells by inducing hemeoxygenase-1 expression via the Nrf2/ARE pathway. Food Chem Toxicol. 2014;69:330-338. [Content Brief]
[2]. Itoh T, et al. Reduced scytonemin isolated from Nostoc commune induces autophagic cell death in human T-lymphoid cell line Jurkat cells. Food Chem Toxicol. 2013;60:76-82. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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