1. Epigenetics
    TGF-beta/Smad
    Stem Cell/Wnt
    Neuronal Signaling
    Protein Tyrosine Kinase/RTK
    Autophagy
    Anti-infection
  2. PKC
    PKA
    CaMK
    Trk Receptor
    Autophagy
    Antibiotic
  3. K-252a

K-252a  (Synonyms: SF2370; Antibiotic K 252a; Antibiotic SF 2370)

Cat. No.: HY-N6732 Purity: 99.27%
COA Handling Instructions

K-252a, a staurosporine analog, inhibits protein kinase, with IC50 values of 470 nM, 140 nM, 270 nM, and 1.7 nM for PKC, PKA, Ca2+/calmodulin-dependent kinase type II, and phosphorylase kinase, respectively. K-252a is a potent inhibitor (IC50 of 3 nM) of the tyrosine protein kinase (TRK) activity of the NGF receptor gp140trk, the product of the trk protooncogene.

For research use only. We do not sell to patients.

K-252a Chemical Structure

K-252a Chemical Structure

CAS No. : 99533-80-9

Size Price Stock Quantity
Solution
10 mM * 1 mL in DMSO USD 813 In-stock
Estimated Time of Arrival: December 31
Solid + Solvent
10 mM * 1 mL
ready for reconstitution
USD 813 In-stock
Estimated Time of Arrival: December 31
Solid
1 mg USD 330 In-stock
Estimated Time of Arrival: December 31
5 mg USD 790 In-stock
Estimated Time of Arrival: December 31
10 mg USD 1260 In-stock
Estimated Time of Arrival: December 31
50 mg   Get quote  
100 mg   Get quote  

* Please select Quantity before adding items.

Customer Review

Based on 9 publication(s) in Google Scholar

Top Publications Citing Use of Products
  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

K-252a, a staurosporine analog, inhibits protein kinase, with IC50 values of 470 nM, 140 nM, 270 nM, and 1.7 nM for PKC, PKA, Ca2+/calmodulin-dependent kinase type II, and phosphorylase kinase, respectively[1][2]. K-252a is a potent inhibitor (IC50 of 3 nM) of the tyrosine protein kinase (TRK) activity of the NGF receptor gp140trk, the product of the trk protooncogene[3].

IC50 & Target

CaMK II

 

In Vitro

K-252a (3-100 nM, 8d) inhibits NGF-promoted neurite outgrowth[5].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis[4].

Cell Line: LINC00641-overexpressed cell line.
Concentration: 1.7 nM (NGF (50 ng/mL)).
Incubation Time: 6 h.
Result: Decreased p-Akt and p-TrkB levels.

Cell Viability Assay[5].

Cell Line: PC12 subclone h cells.
Concentration: 3 to 100 nM.
Incubation Time: 8 d.
Result: Inhibited NGF-promoted neurite outgrowth.
In Vivo

K252a (20 mg/kg/day) inhibiting TrkB pathway dampens the neuroprotective effects induced by TH[6].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Mice[6].
Dosage: 20 mg/kg/day.
Administration: Intraperitoneally, daily, for consecutive 5 days.
Result: Dampened the neuroprotective effects induced by TH.
Molecular Weight

467.47

Formula

C27H21N3O5

CAS No.
SMILES

O=C(NC1)C2=C1C3=C4C5=C2C6=C(C=CC=C6)N5[[email protected]](C[[email protected]]7(O)C(OC)=O)([H])O[[email protected]]7(C)N4C8=C3C=CC=C8

Structure Classification
Source

Nocardiopsis sp.

Shipping

Room temperature in continental US; may vary elsewhere.

Storage
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
Solvent & Solubility
In Vitro: 

DMSO : 50 mg/mL (106.96 mM; Need ultrasonic and warming)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 2.1392 mL 10.6959 mL 21.3917 mL
5 mM 0.4278 mL 2.1392 mL 4.2783 mL
10 mM 0.2139 mL 1.0696 mL 2.1392 mL
*Please refer to the solubility information to select the appropriate solvent.
In Vivo:
  • 1.

    Add each solvent one by one:  10% DMSO    40% PEG300    5% Tween-80    45% saline

    Solubility: ≥ 2.5 mg/mL (5.35 mM); Clear solution

  • 2.

    Add each solvent one by one:  10% DMSO    90% corn oil

    Solubility: ≥ 2.08 mg/mL (4.45 mM); Clear solution

*All of the co-solvents are available by MCE.
Purity & Documentation

Purity: 99.45%

References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email address *

Phone number *

 

Organization name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
K-252a
Cat. No.:
HY-N6732
Quantity:
MCE Japan Authorized Agent: