Obafluorin
Based on 1 Customer Validation
Obafluorin is a β-lactone antibiotic. Obafluorin inhibits threonyl-tRNA synthetase (PfThrRS) with an IC50 of 4.3 nM. Obafluorin inhibits the aminoacylation activity of EcThrRS and ObaO. Obafluorin exhibits Fe3+-enhanced antibacterial activity. Obafluorin can be used in studies related to infections caused by bacterial and parasites such as Plasmodium.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.9%
- CAS No.: 92121-68-1
- 화학식: C17H14N2O7
- 분자량:358.30
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보관:Powder -20°C, 3 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Biological Activity
Obafluorin (Compound 1) (16-18 h) inhibits the growth of B. subtilis (MIC 4 μg/mL), MRSA (MIC 2 μg/mL), E. coli ATCC 25922 (MIC 256 μg/mL) and E. coli NR698 (MIC 4 μg/mL) in the spot lawn antimicrobial assay[1].
Obafluorin (0-5000 nM; 10 min preincubation, up to 10 min reaction) inhibits the aminoacylation activity of purified EcThrRS and ObaO, with IC50 values of 92 nM and 50 nM, respectively[1].
Obafluorin (0-5000 nM; 10 min preincubation; 10 min assay incubation) potently inhibits EcThrRS and PfThrRS in vitro, with IC50 values of 35 nM and 4.3 nM, respectively[2].
The antibacterial activity of Obafluorin (16-18 h; 2 mM Fe3+ added to agar) is significantly enhanced in the presence of Fe3+: the MIC against E. coli ATCC 25922 decreases by ≥256-fold, the MIC against P. aeruginosa PA01 decreases by ≥128-fold, and the MIC against MRSA decreases by 8-fold[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 92121-68-1
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Appearance Solid
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분자량 358.30
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화학식 C17H14N2O7
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Color Light yellow to yellow
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SMILES
O=C(C(C=CC=C1O)=C1O)N[C@H](C2=O)[C@H](O2)CC(C=C3)=CC=C3[N+]([O-])=O
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years In solvent -80°C 6 months -20°C 1 month
용액&용해도
MeCN : 1 mg/mL (2.79 mM; Need ultrasonic and warming)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
순도&문서
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Data Sheet (281 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Scott TA, et al. Immunity-Guided Identification of Threonyl-tRNA Synthetase as the Molecular Target of Obafluorin, a β-Lactone Antibiotic. ACS Chem Biol. 2019;14(12):2663-2671. [Content Brief]
[2]. Batey SFD, et al. The catechol moiety of obafluorin is essential for antibacterial activity. RSC Chem Biol. 2023;4(11):926-941. Published 2023 Aug 21. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| MeCN | 1 mM | 2.7910 mL | 13.9548 mL | 27.9096 mL | 69.7739 mL |
Obafluorin Related Classifications
HY-138075 Related Classifications
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