Cat. No.: HY-149265

ROS-generating agent 1

고객리뷰

Based on 1 Customer Validation

ROS-generating agent 1 is a selective ROS-generating agent. ROS-generating agent 1 inhibits TrxR activity and expression in cancer cells. ROS-generating agent 1 induces ROS-dependent apoptosis and ferroptosis in cancer cells. ROS-generating agent 1 selectively kills lung cancer cells and inhibits the growth of cancer cell xenograft tumors in nude mice. ROS-generating agent 1 can be used for the research of non-small cell lung cancer.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.11%
CAS No.: 2369030-41-9
화학식: C₂₁H₁₅F₆NO
분자량:411.34

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ROS-generating agent 1 Chemical Structure

CAS No.: 2369030-41-9

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
용액&용해도
- In Vitro
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

All TrxR Isoforms :

TrxR1
TrxR2

All Caspase Isoforms :

All Bcl-2 Family Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	IC₅₀	5.8 μM Compound: 2c	Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human A549 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36758308]
HepG2	IC₅₀	0.67 μM Compound: 2c	Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36758308]
HT-1080	IC₅₀	0.93 μM Compound: 2c	Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human HT-1080 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36758308]
MRC5	IC₅₀	14.09 μM Compound: 2c	Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human MRC5 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36758308]
NCI-H460	IC₅₀	0.44 μM Compound: 2c	Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay	[PMID: 36758308]

In Vitro

ROS-generating agent 1 (compound 2c) (48 h) potently and selectively inhibits the viability of NCI-H460 cells (IC₅₀ = 0.44 μM) over normal MRC-5 cells, with a selectivity index of 32.0, and also shows strong cytotoxicity against A549, HepG2, and HT-1080 cancer cells^[1].
ROS-generating agent 1 (0.5-2 μM; 1-12 h) selectively induces time- and concentration-dependent ROS accumulation in NCI-H460 cells but not in normal MRC-5 cells^[1].
ROS-generating agent 1 (9 h) selectively depletes intracellular GSH levels in NCI-H460 cells in a dose-dependent manner, while having no significant effect on MRC-5 cells^[1].
ROS-generating agent 1 (0.5-2 μM; 6 h) dose-dependently inhibits TrxR activity in NCI-H460 cells but has negligible effect on TrxR activity in normal MRC-5 cells^[1].
ROS-generating agent 1 (0.5-2 μM; 6 h) potently and dose-dependently inhibits TrxR activity in NCI-H460 cells, with 2 μM causing 59% inhibition^[1].
ROS-generating agent 1 (0.5-2 μM; 6 h) dose-dependently reduces intracellular TrxR protein levels in NCI-H460 cells, with 2 μM causing 69% suppression^[1].
ROS-generating agent 1 covalently binds to the Sec-498 residue of human TrxR1 with a docking affinity score of −5.494 kcal/mol, forming hydrogen bonds and a cation-π interaction with key active site residues^[1].
ROS-generating agent 1 (0.5-1 μM; 48 h) kills NCI-H460 cells via induction of apoptosis and ferroptosis^[1].
ROS-generating agent 1 (0.5-2 μM; 12-36 h) concentration- and time-dependently reduces mitochondrial membrane potential in NCI-H460 cells^[1].
ROS-generating agent 1 (1-2 μM; 48 h) induces ROS-dependent mitochondrial-mediated apoptosis in NCI-H460 cells, as shown by altered expression of Bcl-2, Bax, caspase-9, and caspase-3^[1].
ROS-generating agent 1 (0.25-0.5 μM; 40 h) selectively induces ferroptosis in a dose-dependent manner in NCI-H460 cells^[1].
ROS-generating agent 1 (0.5-2 μM; 40 h) dose-dependently increases MDA levels in NCI-H460 cells^[1].
ROS-generating agent 1 (0.5-2 μM; 24 h) dose-dependently reduces GPX4 protein levels in NCI-H460 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

NCI-H460 cells
Concentration:

0.5; 1; 2 μM
Incubation Time:

6 h
Result:

Inhibited intracellular TrxR protein expression in a dose-dependent manner, with 2 μM suppressing 69% of TrxR expression.

Cell Viability Assay^[1]

Cell Line:

NCI-H460 cells
Concentration:

0.5; 1 μM
Incubation Time:

48 h
Result:

Dose-dependently reduced cell viability of NCI-H460 cells.

Western Blot Analysis^[1]

Cell Line:

NCI-H460 cells
Concentration:

1; 2 μM
Incubation Time:

48 h
Result:

Triggered down-regulation of anti-apoptotic Bcl-2, up-regulation of pro-apoptotic Bax, and activation of caspase-9 and caspase-3.
These changes were effectively reversed by pretreatment with NAC.

Western Blot Analysis^[1]

Cell Line:

NCI-H460 cells
Concentration:

0.5; 1; 2 μM
Incubation Time:

24 h
Result:

Reduced GPX4 protein expression in a concentration-dependent manner.

In Vivo

ROS-generating agent 1 (compound 2c) (5-15 mg/kg; i.p.; every 2 days; 4 weeks) dose-dependently inhibits non-small cell lung cancer xenograft growth in BALB/c nude mice^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

BALB/c nude mice (male, 5-6 weeks old) subcutaneously inoculated with NCI-H460 cells^[1]
Dosage:

5; 15 mg/kg
Administration:

i.p.; every 2 days; 4 weeks
Result:

Significantly and dose-dependently inhibited NCI-H460 xenograft tumor growth.
Showed no significant changes in body weight.
Exhibited no abnormal histology in liver or kidney tissues via H&E staining.
Increased tumor cell apoptosis.
Down-regulated GPX4 expression in tumor tissues compared to controls.

Chemical Information

CAS No. 2369030-41-9
Appearance Solid
분자량 411.34
화학식 C₂₁H₁₅F₆NO
Color Light yellow to yellow
SMILES

O=C1/C(CNC/C1=C\C2=CC(C(F)(F)F)=CC=C2)=C/C3=CC=CC(C(F)(F)F)=C3
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 33.33 mg/mL (81.03 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.4311 mL

12.1554 mL

24.3108 mL

5 mM

0.4862 mL

2.4311 mL

4.8622 mL

10 mM

0.2431 mL

1.2155 mL

2.4311 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (275 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Liu X, et al. Tumor killing by a dietary curcumin mono-carbonyl analog that works as a selective ROS generator via TrxR inhibition. Eur J Med Chem. 2023 Mar 15;250:115191. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.4311 mL	12.1554 mL	24.3108 mL	60.7770 mL
	5 mM	0.4862 mL	2.4311 mL	4.8622 mL	12.1554 mL
	10 mM	0.2431 mL	1.2155 mL	2.4311 mL	6.0777 mL
	15 mM	0.1621 mL	0.8104 mL	1.6207 mL	4.0518 mL
	20 mM	0.1216 mL	0.6078 mL	1.2155 mL	3.0388 mL
	25 mM	0.0972 mL	0.4862 mL	0.9724 mL	2.4311 mL
	30 mM	0.0810 mL	0.4052 mL	0.8104 mL	2.0259 mL
	40 mM	0.0608 mL	0.3039 mL	0.6078 mL	1.5194 mL
	50 mM	0.0486 mL	0.2431 mL	0.4862 mL	1.2155 mL
	60 mM	0.0405 mL	0.2026 mL	0.4052 mL	1.0129 mL
	80 mM	0.0304 mL	0.1519 mL	0.3039 mL	0.7597 mL

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ROS-generating agent 1

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Ferroptosis Compound Library

Mitochondria-Targeted Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

All TrxR Isoforms

All Caspase Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

ROS-generating agent 1

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Anti-Cancer Compound Library

Anti-Aging Compound Library

Ferroptosis Compound Library

Mitochondria-Targeted Compound Library

Metabolic Enzyme Compound Library

Highly Selective Inhibitors Library

Highly Selective Activators Library

Cell Death Library

Multi-Target Compound Library

Mitophagy Compound Library

Bioactive Compound Library Max

Bioactive Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

High-Throughput Bioactive Compound Library

Lactylation Compound Library

All TrxR Isoforms

All Caspase Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

ROS-generating agent 1 Related Classifications

HY-149265 Related Classifications