2. NF-κB MAPK/ERK Pathway PI3K/Akt/mTOR Immunology/Inflammation Metabolic Enzyme/Protease Apoptosis
  3. IKK NF-κB p38 MAPK PI3K Akt Reactive Oxygen Species (ROS) Interleukin Related TNF Receptor Apoptosis Caspase Bcl-2 Family
  4. 4′-Hydroxywogonin

4′-Hydroxywogonin  (Synonyms: 8-Methoxyapigenin)

Cat. No.: HY-N1904 Purity: 98.22%
COA
SDS
Handling Instructions Technical Support

4′-Hydroxywogonin (8-Methoxyapigenin), a flavonoid, could be isolated from a variety of plants including Scutellaria barbata and Verbena littoralis. 4′-Hydroxywogonin has anti-inflammatory activity via TAK1/IKK/NF-κB, MAPKs and PI3/AKT signaling pathways. 4′-Hydroxywogonin inhibits angiogenesis by disrupting PI3K/AKT signaling. 4′-Hydroxywogonin inhibits cell proliferation and induces apoptosis.

For research use only. We do not sell to patients.

4′-Hydroxywogonin

4′-Hydroxywogonin Chemical Structure

CAS No. : 57096-02-3

Size Price Stock Quantity
1 mg In-stock
5 mg In-stock
10 mg   Get quote  
50 mg   Get quote  

* Please select Quantity before adding items.

This product is a controlled substance and not for sale in your territory.

Customer Review

Based on 1 Customer Validation

4′-Hydroxywogonin Related Antibodies

Top Publications Citing Use of Products

View All IKK Isoform Specific Products:

View All Isoforms
IKK-α IKK-β IKK IKKε TBK1

View All NF-κB Isoform Specific Products:

View All Isoforms
NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

View All p38 MAPK Isoform Specific Products:

View All Isoforms
p38 MAPK Akt1 p38α p38β MAPK13 p38δ p38γ

View All PI3K Isoform Specific Products:

View All Isoforms
PI3Kα PI3Kβ PI3Kγ PI3Kδ PI3KC2α PI3KC2β PI3KC2γ Vps34 PI3K PI3KC3 p120γ

View All Akt Isoform Specific Products:

View All Isoforms
Akt Akt1 Akt2 Akt3

View All Interleukin Related Isoform Specific Products:

View All Isoforms
IL-1 IL-2 IL-3 IL-4 IL-5 IL-6 IL-8 IL-10 IL-12 IL-13 IL-15 IL-17 IL-23 IL-7 IL-33 IL-22 IL-18

View All TNF Receptor Isoform Specific Products:

View All Isoforms
TNFRSF1A/CD120a TNFRSF3/CD18 TNFRSF4 TNFRSF5/CD40 TNFRSF6/Fas/CD95 TNFRSF7/CD27 TNFRSF8/CD30 TNFRSF9/4-1BB/CD137 TNFRSF10B/DR5/CD262 TNFRSF12A/TWEAK TNFRSF16/NGF Receptor/CD271 TNFRSF18/GITR/CD357

View All Caspase Isoform Specific Products:

View All Isoforms
Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

View All Bcl-2 Family Isoform Specific Products:

View All Isoforms
Bcl-2 Bcl-xL Bcl-W Mcl-1 Bfl-1 Bcl-B Bax Bak Bim BNIP3 Bad

  • Biological Activity

  • Purity & Documentation

  • References

  • Customer Review

Description

4′-Hydroxywogonin (8-Methoxyapigenin), a flavonoid, could be isolated from a variety of plants including Scutellaria barbata and Verbena littoralis. 4′-Hydroxywogonin has anti-inflammatory activity via TAK1/IKK/NF-κB, MAPKs and PI3/AKT signaling pathways. 4′-Hydroxywogonin inhibits angiogenesis by disrupting PI3K/AKT signaling. 4′-Hydroxywogonin inhibits cell proliferation and induces apoptosis[1][2][3].

Cellular Effect
Cell Line Type Value Description References
RAW264.7 IC50
12.5 μM
Compound: 11
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by Griess reaction based method
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced nitric oxide production by Griess reaction based method
[PMID: 25637363]
In Vitro

4′-Hydroxywogonin (8-Methoxyapigenin; 0.5-15 μM; 0-24 h) has low cytotoxicity and inhibits NO and PGE2 production in LPS-stimulated RAW 264.7 macrophages by suppression of iNOS and COX-2 expression[1].
4′-Hydroxywogonin (0.5-15 μM; 1 and 12 h) suppresses LPS-induced expression of pro-inflammatory cytokines in RAW 264.7 macrophages and suppresses LPS-induced activation of NF-κB[1].
4′-Hydroxywogonin (0.5-15 μM; 1 h) suppresses LPS-induced degradation of IκB-α and activation of IKK and TAK and suppresses the phosphorylation of MAPK and AKTin in RAW 264.7 macrophages[1].
4′-Hydroxywogonin (0.5-15 μM; 24 h) inhibits ROS production in LPS-stimulated RAW 264.7 macrophages[1].
4′-Hydroxywogonin (0-10 μg/mL; 24 h) reduces the viability of SW620 cells in a concentration- and time-dependent manner and decreases the mRNA and protein expression of vascular endothelial growth factor-A (VEGF-A), the predominant pro-angiogenic cytokine in tumor angiogenesis[2].
4′-Hydroxywogonin (24 h; SUP-B15 and Jurkat cells) induces apoptosis and decreases the expression of C-MYC, BCL-2 and cleaved caspase 3[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

4′-Hydroxywogonin Related Antibodies

Cell Viability Assay[1]

Cell Line: RAW 264.7 macrophages
Concentration: 0.5, 5 and 15 μM
Incubation Time: 24 hours
Result: Had low cytotoxicity in RAW 264.7 macrophages.

Western Blot Analysis[1]

Cell Line: RAW 264.7 macrophages
Concentration: 0.5, 5 and 15 μM
Incubation Time: 1 hours
Result: Attenuated the increase of iNOS and COX-2 mRNA expression induced by LPS in RAW 264.7 cells.

Western Blot Analysis[1]

Cell Line: RAW 264.7 macrophages
Concentration: 0.5, 5 and 15 μM
Incubation Time: 1 and 12 hours
Result: Reduced TNF-α, IL-6 and IL-1β mRNA expression in a dose-dependent manner.
Inhibited LPS-induced p65 phosphorylation and nuclear translocation.

Western Blot Analysis[1]

Cell Line: RAW 264.7 macrophages
Concentration: 0.5, 5 and 15 μM
Incubation Time: 1 hours
Result: Attenuated LPS induced IκB-α degradation.
Attenuated the phosphorylation of ERK1/2 and p38 in a dose-dependent manner.
Reduced the intensity of the TAK1/TAB1 band.

Western Blot Analysis[2]

Cell Line: SW620 cells
Concentration: 0.1, 1, and 10 μg/mL
Incubation Time: 24 hours
Result: Downregulated VEGF-Aexpression in colorectal cancer cells and suppressed angiogenesis.
In Vivo

4′-Hydroxywogonin (10 and 20 mg/kg; i.p.; male C57BL/6 mice) alleviates LPS-induced acute lung injury (ALI) in a mouse model[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male C57BL/6 mice (6-8 weeks old; 20 g) with acute lung injury model[1]
Dosage: 10 and 20 mg/kg
Administration: Intraperitoneal injection, 12 and 1 h before LPS treatment
Result: Had potential protective effects against inflammation in LPS induced ALI mice.
Attenuated the degree of leukocyte infiltration.
Molecular Weight

300.26

Formula

C16H12O6
CAS No.
Appearance

Solid

Color

Brown to breen

SMILES

O=C1C=C(C2=CC=C(O)C=C2)OC3=C(OC)C(O)=CC(O)=C13
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, protect from light

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Purity & Documentation
References
  • No file chosen (Maximum size is: 1024 Kb)
  • If you have published this work, please enter the PubMed ID.
  • Your name will appear on the site.

4′-Hydroxywogonin Related Classifications

  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

4′-Hydroxywogonin57096-02-38-MethoxyapigeninIKKNF-κBp38 MAPKPI3KAktReactive Oxygen Species (ROS)Interleukin RelatedTNF ReceptorApoptosisCaspaseBcl-2 FamilyIκB kinaseI kappa B kinase Nuclear factor-κBNuclear factor-kappaBPhosphoinositide 3-kinasePKBProtein kinase BILTumor Necrosis Factor ReceptorTNFRflavonoidScutellaria barbataVerbena littoralisanti-inflammatoryangiogenesisantitumorInhibitorinhibitorinhibit

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

  

Requested Quantity *

Applicant Name *

 

Salutation

Email Address *

 

Phone Number *

Department

 

Organization Name *

City

State

Country or Region *

      

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
4′-Hydroxywogonin
Cat. No.:
HY-N1904
Quantity:
MCE Japan Authorized Agent:

Customer Review

Thank You for Your Feedback!

Request for HNMR Report

Please fill out this form to request the QC report. We will send it to your Email address shortly.

Your information is safe with us. * Required Fields.

Product Name

  

Lot #

Applicant Name *

 

Email Address *

Phone Number

 

Department *

Organization Name *

 

Country or Region *

Remarks

SAFETY DATA SHEET (SDS)

English - EN (393 KB) Français - FR (393 KB) Deutsch - DE (393 KB) Norwegian - NO (393 KB) Español - ES (393 KB) Swedish - SV (393 KB) Italian - IT (393 KB) Korean - KR (393 KB) Portuguese - PT (393 KB)

Request for HNMR Report

We have received your request and will respond to you as soon as possible.