Art. -Nr.: HY-W010155

Tryptophol

(Synonyms: Indole-3-ethanol)

2 Cited Publications

Kundenbewertung

Based on 2 publication(s) in Google Scholar

Tryptophol is an aromatic alcohol and secondary metabolite produced by microorganisms. Tryptophol induces apoptosis and cleavage of caspase-8. Tryptophol inhibits Cunninghamella blakesleeana biofilm. Tryptophol has anti-phage infection, biofilm formation regulation, anti-inflammatory, hemolytic, sleep induction, temperature change, seizure susceptibility and immune regulation activities. Tryptophol is used in the research of African trypanosomiasis, sleep disorders, epilepsy.

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Reinheit: 99.80%
CAS. Nr.: 526-55-6
Formel: C₁₀H₁₁NO
Molecular Weight:161.20

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Tryptophol Chemical Structure

CAS. Nr.: 526-55-6

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Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]^[2]^[3]^[4]^[5]^[6]^[7]^[8]^[9]^[10]^[11]^[12]

Microbial Metabolite

Caspase-8

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen :

Alle Caspase Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
HaCaT	IC₅₀	6.55 μM Compound: 9	Antiinflammatory activity against human HaCaT cells assessed as inhibition of UVB irradiation-induced PGE2 production measured after 2 hrs by ELISA Antiinflammatory activity against human HaCaT cells assessed as inhibition of UVB irradiation-induced PGE2 production measured after 2 hrs by ELISA	[PMID: 35172097]
U-251	EC₅₀	1220 μM Compound: 8	Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U251 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]
U-87MG ATCC	EC₅₀	580 μM Compound: 8	Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay Cytotoxicity against human U87 cells assessed as decrease in cell proliferation after 72 hrs by SRB assay	[PMID: 28256372]

In Vitro

Tryptophol (25 μM) promotes the population density, biofilm formation and cell aggregation of the marine yeast Scheffersomyces spartinae and enhances its biocontrol effect against strawberry gray mold (Botrytis cinerea)^[1].
. Tryptophol (2 mM; 24 h) causes DNA damage in HepG2, A549 and THP-1 cells^[2].
Tryptophol (1-100 μM) inhibits the infection and replication of bacteriophage GVE2 against the thermophilic bacterium Geobacillus sp. E263^[3].
Tryptophol (0.25-2.00 mM; 24 h) is cytotoxic and genotoxic to human peripheral blood lymphocytes^[4].
Tryptophol (80 μg/ml; 12 h) induces apoptosis in U937 cells^[5].
Tryptophol (25-100 μM; 24 h) has anti-inflammatory activity in 3T3-L1 mouse adipocytes in vitro and reduces MCP-1 production in a dose-dependent manner^[6].
Tryptophol (0.02-0.1 mg/mL; 96 h) inhibits Cunninghamella blakesleeana biofilm growth and causes shedding of established biofilms^[7].
Tryptophol (3.8 mg/mL) lyses human red blood cells in pH and temperature- dependent manner^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[5]

Cell Line:

U937
Concentration:

80 μg/ml
Incubation Time:

12 h
Result:

Led to apoptosis.
Induced cleavage of caspase-8 and the formation of a death-inducing signaling complex including DR5.

In Vivo

Tryptophol (100-300 mg/kg; i.p.) induces a sleep-like state and causes changes in body temperature in mice^[8].
Tryptophol (200-600 mg/kg; i.p.) induces sleep in mice in a dose-dependent manner^[9].
Tryptophol (50-100 mg/kg; i.p.; twice daily; 3 days) combined with ethanol increases the seizure susceptibility of mice^[10].
Tryptophol (200 mg/kg; i.p.) effectively suppresses Pentylenetetrazol and Picrotoxin (HY-101391) induced convulsion in mice^[11].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

CD-1 strain male mice (male, 16-30 g) for sleep induction study^[9]
Dosage:

200, 400, 600 mg/kg
Administration:

Intraperitoneal injection (dissolved in 50% propylene glycol- water, sesame oil or Tween 80)
Result:

Induced sleep in mice.
Induced sleep in 8 of 12 mice, with an onset time of 2.8 min and sleep duration of 4.2 min (at 200 mg/kg) (dissolved in 50% propylene glycol-water).
Induced sleep in 10 of 12 mice, with an onset time of 1.4 min and sleep duration of 14.4 min (at 400 mg/kg) (dissolved in 50% propylene glycol-water).
Induced sleep in 3 out of 6 mice, with an onset time of 2.3 min and a sleep duration of 5.2 min (dissolved in sesame oil, at 200 mg/kg).
Induced sleep in 6 out of 6 mice, with an onset time of 1.8 min and a sleep time of 16.7 min (dissolved in sesame oil, at 400 mg/kg).
Induced sleep in 5 out of 6 mice, with an onset time of 2.1 min and a sleep time of 4.8 min (dissolved in Tween 80, at 200 mg/kg).
Induced sleep in 5 out of 6 mice, with an onset time of 1.7 min and a sleep time of 15.7 min (dissolved in Tween 80, at 400 mg/kg).

Chemical Information

CAS. Nr. 526-55-6
Appearance Solid
Molecular Weight 161.20
Formel C₁₀H₁₁NO
Color Light yellow to brown
SMILES

OCCC1=CNC2=CC=CC=C12
Synonyms

Indole-3-ethanol
Structure Classification
- Others
Initial Source
- Endogenous metabolite
- Human Gut Microbiota Metabolites
- Microorganisms
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

All Bio/Physico-chemical AssayIn Vivo Efficacy StudyCell Imaging/StainingRT-PCRWB

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Journal Impact Factor

Adv Sci (Weinh)

IL4I1⁺ Macrophages and TDO2⁺ Myofibroblasts Drive AhR-Mediated Immunosuppression and Ferroptosis Resistance in Solid Predominant Lung Adenocarcinoma. [Abstract]

2025 Dec 22:e13606. PMID: 41431173
Phytomedicine

A microbiota-tryptophol-AhR axis mediates the gut-kidney protective effects of Hushen Tongfengtai Granules in hyperuricemic nephropathy. [Abstract]

2026 Jun:155:158089. PMID: 41886955

Tryptophol purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jun:155:158089. [Abstract]

Feature ranking using Random Forest and Support Vector Machine (SVM) algorithms identifying top metabolites contributing to group discrimination, with tryptophol (indole-3-ethanol) highlighted.

Tryptophol purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jun:155:158089. [Abstract]

Schematic illustration of the urate nephropathy induction and treatment protocol. Mice received daily intraperitoneal injections of potassium oxonate (PO, 300 mg/kg) and oral hypoxanthine (Hx, 250 mg/kg) for 14 days, During the same treatment with medium-dose HSTFT (HSTFT-M, 33.47 g/kg) or tryptophol at low (TOL-L, 50 mg/kg/day) and high (TOL-H, 100 mg/kg/day) doses (n=6 per group).

Tryptophol purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jun:155:158089. [Abstract]

AB-PAS staining further showed marked restoration of goblet-cell abundance and mucin secretion, particularly in the high-dose Tryptophol (TOL) group.

Tryptophol purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jun:155:158089. [Abstract]

Colonic expression of IL6, TNFα, and IL1β were significantly suppressed following Tryptophol (TOL) administration, confirming its potent anti-inflammatory effect.

Tryptophol purchased from MedChemExpress. Usage Cited in: Phytomedicine. 2026 Jun:155:158089. [Abstract]

Western blot analysis confirmed these results at the protein level. Compared with the model group, Tryptophol (TOL)-treated mice exhibited significantly higher expression of tightjunction proteins ZO-1 and Occludin, as well as adherens junction marker E-cadherin.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (620.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.2035 mL

31.0174 mL

62.0347 mL

5 mM

1.2407 mL

6.2035 mL

12.4069 mL

10 mM

0.6203 mL

3.1017 mL

6.2035 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 3.25 mg/mL (20.16 mM); Clear solution

This protocol yields a clear solution of ≥ 3.25 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (32.5 mg/mL) to 900 μL Corn oil, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (15.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (15.51 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 66.67 mg/mL (413.59 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (295 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Zhao Z, et al. Tryptophol Improves the Biocontrol Efficacy of Scheffersomyces spartinae against the Gray Mold of Strawberries by Quorum Sensing. J Agric Food Chem. 2023 Dec 13;71(49):19739-19748. [Content Brief]

[2]. Kosalec I, et al. Assessment of tryptophol genotoxicity in four cell lines in vitro: a pilot study with alkaline comet assay. Arh Hig Rada Toksikol. 2011 Mar;62(1):41-9. [Content Brief]

[3]. Jin M, et al. The effect of tryptophol on the bacteriophage infection in high-temperature environment. Appl Microbiol Biotechnol. 2015 Oct;99(19):8101-11. [Content Brief]

[4]. Kosalec I, et al. Genotoxicity of tryptophol in a battery of short-term assays on human white blood cells in vitro. Basic Clin Pharmacol Toxicol. 2008 May;102(5):443-52. [Content Brief]

[5]. Inagaki S, et al. Tryptophol induces death receptor (DR) 5-mediated apoptosis in U937 cells. Biosci Biotechnol Biochem. 2007 Aug;71(8):2065-8. [Content Brief]

[6]. Niwa T, et al. In Vitro Anti-inflammatory Activity of Tyrosol and Tryptophol: Metabolites of Yeast via the Ehrlich Pathway. Biol Pharm Bull. 2025;48(2):115-118. [Content Brief]

[7]. Khan MF, et al. Regulation of Cunninghamella spp. biofilm growth by tryptophol and tyrosol. Biofilm. 2021 Mar 26;3:100046. [Content Brief]

[8]. Seed JR, et al. The biological effects of tryptophol (indole-3-ethanol): hemolytic, biochemical and behavior modifying activity. Comp Biochem Physiol C Comp Pharmacol. 1978;60(2):175-85. [Content Brief]

[9]. Feldstein A, et al. Tryptophol, 5-hydroxytryptophol and 5-methoxytryptophol induced sleep in mice. Life Sciences, 1970, 9(6): 323-329.

[10]. FUKUMORI R, et al. Changes in seizure susceptibility after successive treatments of mice with tryptophol and ethanol. Journal of Pharmacy and Pharmacology, 1981, 33(1): 586-589.

[11]. Satoh T, et al. Effect of tryptophol on pentylenetetrazol and picrotoxin induced convulsion in mice. Life Sci. 1979 May 28;24(22):2031-6. [Content Brief]

[12]. Park I, et al. Gut Microbiota-Derived Indole-3-Carboxylate Influences Mucosal Integrity and Immunity Through the Activation of the Aryl Hydrocarbon Receptors and Nutrient Transporters in Broiler Chickens Challenged With Eimeria maxima. Front Immunol. 2022 Jun 23;13:867754. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.2035 mL	31.0174 mL	62.0347 mL	155.0869 mL
	5 mM	1.2407 mL	6.2035 mL	12.4069 mL	31.0174 mL
	10 mM	0.6203 mL	3.1017 mL	6.2035 mL	15.5087 mL
	15 mM	0.4136 mL	2.0678 mL	4.1356 mL	10.3391 mL
	20 mM	0.3102 mL	1.5509 mL	3.1017 mL	7.7543 mL
	25 mM	0.2481 mL	1.2407 mL	2.4814 mL	6.2035 mL
	30 mM	0.2068 mL	1.0339 mL	2.0678 mL	5.1696 mL
	40 mM	0.1551 mL	0.7754 mL	1.5509 mL	3.8772 mL
	50 mM	0.1241 mL	0.6203 mL	1.2407 mL	3.1017 mL
	60 mM	0.1034 mL	0.5170 mL	1.0339 mL	2.5848 mL
	80 mM	0.0775 mL	0.3877 mL	0.7754 mL	1.9386 mL
	100 mM	0.0620 mL	0.3102 mL	0.6203 mL	1.5509 mL

Keywords

Tryptophol

2 Cited Publications

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Tryptophol

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Publications Citing Use of MedChemExpress (MCE) Tryptophol

Alle Endogenous Metabolite Isoform-spezifische Produkte anzeigen

Alle Caspase Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (2)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Tryptophol Related Classifications

Art. -Nr.: HY-W010155