Art. -Nr.: HY-13428

Tubacin

25 Cited Publications

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Based on 25 publication(s) in Google Scholar

Tubacin is a potent and selective inhibitor of HDAC6, with an IC₅₀ value of 4 nM and approximately 350-fold selectivity over HDAC1. Tubacin also inhibits metallo-β-lactamase domain-containing protein 2 (MBLAC2).

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Reinheit: 97.11%
CAS. Nr.: 537049-40-4
Formel: C₄₁H₄₃N₃O₇S
Molecular Weight:721.86

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 1 year , -20°C, 6 months

Tubacin Chemical Structure

CAS. Nr.: 537049-40-4

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Publications (25)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Protokoll
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

HDAC6

4 nM (IC₅₀)

HDAC3

1.27 μM (IC₅₀)

HDAC8

1.27 μM (IC₅₀)

HDAC1

1.40 μM (IC₅₀)

HDAC5

3.35 μM (IC₅₀)

HDAC10

3.71 μM (IC₅₀)

HDAC11

3.79 μM (IC₅₀)

HDAC9

4.31 μM (IC₅₀)

HDAC2

6.27 μM (IC₅₀)

HDAC7

9.70 μM (IC₅₀)

HDAC4

17.30 μM (IC₅₀)

Alle Beta-lactamase Isoform-spezifische Produkte anzeigen :

Alle HDAC Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
A549	EC₅₀	2.5 μM Compound: 38	Inhibition of HDAC-6 in human A549 cells assessed as induction of alpha-tubulin acetylation after 20 hrs by cytoblot analysis Inhibition of HDAC-6 in human A549 cells assessed as induction of alpha-tubulin acetylation after 20 hrs by cytoblot analysis	10.1039/C1MD00199J
A549	EC₅₀	2.9 μM Compound: Tubacin	Inhibition of HDAC6 in human A549 cells assessed as induction of alpha-tubulin acetylation by fluorescence microscopy Inhibition of HDAC6 in human A549 cells assessed as induction of alpha-tubulin acetylation by fluorescence microscopy	[PMID: 16408003]
HeLa	IC₅₀	2.9 μM Compound: Tubacin	Inhibition of HDAC6 in human HeLa cells using acetyl tubulin as substrate assessed as Ac-Lys accumulation in nuclear histones after 6 hrs by immunofluorescence assay Inhibition of HDAC6 in human HeLa cells using acetyl tubulin as substrate assessed as Ac-Lys accumulation in nuclear histones after 6 hrs by immunofluorescence assay	[PMID: 23964961]
HeLa	IC₅₀	2.9 μM Compound: Tubacin	Inhibition of HDAC6 in human HeLa cells assessed as reduction in K40 hyperacetylation of alpha-tubulin incubated for 6 hrs by immunofluorescence assay Inhibition of HDAC6 in human HeLa cells assessed as reduction in K40 hyperacetylation of alpha-tubulin incubated for 6 hrs by immunofluorescence assay	[PMID: 25454270]
T-24	EC₅₀	2.9 μM Compound: Tubacin	Induction of histone H3 acetylation in human T24 cells Induction of histone H3 acetylation in human T24 cells	[PMID: 19111466]

In Vitro

Tubacin preferentially induces α-tubulin hyperacetylation at a concentration of 2.5 μM, and induces α-tubulin acetylation at 5 μM and protects prostate cancer (LNCaP) cells from hydrogen peroxide-induced death at 8 μM via peroxiredoxin acetylation^[1]. Tubacin (2.5 and 5 μM) specifically induces acetylation of α-tubulin in MM cells. Tubacin significantly inhibits both drug-sensitive and drug-resistant MM cell growth, with IC₅₀ 5-20 μM at 72 h. Tubacin also induces apoptosis by activation of caspases. Moreover, Tubacin inhibits binding of HDAC6 with dynein, and it induces significant accumulation of polyubiquitinated proteins, when combined with bortezomib. Tubacin and bortezomib induce synergistic antitumor activity in MM cell lines, and inhibits paracrine MM Cell Growth. Tubacin (5 μM) synergistically enhances bortezomib-induced cytotoxicity in patient MM cells without cytotoxicity to PBMCs^[2]. Tubacin can concentration-dependently inhibits JEV-induced cytopathic effect and apoptosis, as well as reduces virus yield in human cerebellar medulloblastoma cells. The IC₅₀ of virus yield is 0.26 μM for Tubacin. Tubacin also meaningfully blocks the production of intracellular infectious virus particles, with an IC₅₀ of 1.52 μM. Tubacin induces the hyperacetylation of a HDAC6 substrate Hsp90 and reduces the interaction of Hsp90 with JEV NS5 protein^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS. Nr. 537049-40-4
Appearance Solid
Molecular Weight 721.86
Formel C₄₁H₄₃N₃O₇S
Color White to off-white
SMILES

OCC(C=C1)=CC=C1[C@@H]2C[C@H](CSC3=NC(C4=CC=CC=C4)=C(C5=CC=CC=C5)O3)O[C@H](C6=CC=C(NC(CCCCCCC(NO)=O)=O)C=C6)O2
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	1 year
	-20°C	6 months

Publications (25)

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Journal Impact Factor

Cell

CD36-mediated endocytosis of proteolysis-targeting chimeras. [Abstract]

2025 Jun 12;188(12):3219-3237.e18. PMID: 40250420
Nat Metab

Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation. [Abstract]

2024 Aug;6(8):1505-1528. PMID: 39134903
Cell Stem Cell

Establishing dorsal-ventral patterning in human neural tube organoids with synthetic organizers. [Abstract]

2025 Jul 3;32(7):1071-1086.e8. PMID: 40373768
Nat Commun

Identification of a component of the tubulin poly-glutamylase complex required for phosphoinositide homeostasis and cilium assembly and maintenance. [Abstract]

2026 May 15. PMID: 42140944
Nat Commun

Human iPSC-based Modeling of Pulmonary Fibrosis Reveals p300/CBP Inhibition Suppresses Alveolar Transitional Cell State. [Abstract]

2026 Feb 12;17(1):1214. PMID: 41680175
Nat Commun

LPA signaling acts as a cell-extrinsic mechanism to initiate cilia disassembly and promote neurogenesis. [Abstract]

2021 Jan 28;12(1):662. PMID: 33510165

Tubacin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jan 28;12(1):662. [Abstract]

Quantification of cilium length of Ki67+ cells in Sox2-positive RG cells. Isolated cells from Lpar1^+/+ cerebral cortex were treated with 2 μM tubacin or DMSO for 72 h, and isolated cells from Lpar1^−/− cerebral cortex were infected with lentivirus expressed GFP or GFP-Lpar1 with or without 2 μM tubacin treatment for 72 h.

Tubacin purchased from MedChemExpress. Usage Cited in: Nat Commun. 2021 Jan 28;12(1):662. [Abstract]

Percentage of Ki67+ cells in Sox2-positive RG cells. Isolated cells from Lpar1^+/+ cerebral cortex were treated with 2 μM tubacin or DMSO for 72 h, and isolated cells from Lpar1^−/− cerebral cortex were infected with lentivirus expressed GFP or GFP-Lpar1 with or without 2 μM tubacin treatment for 72 h.
Biomark Res

A novel indirubin- 3-monoxime derivative I3MV- 8b exhibits remarkable cytotoxicity against multiple myeloma by targeting TRIM28. [Abstract]

2025 Apr 7;13(1):57. PMID: 40197552

Tubacin purchased from MedChemExpress. Usage Cited in: Biomark Res. 2025 Apr 7;13(1):57. [Abstract]

Heatmap showing the combination index (CI) values of I3MO and Tubacin (Tub, 3.125-25 μM) in MM cell lines. The cells were treated with various combinations of I3MO and Tub at different doses, and the CI values were calculated using the Chou-Talalay method. The heatmap color scale represents the CI values (indicated on the graph), with red indicating synergistic effect (CI < 1.0).

Tubacin purchased from MedChemExpress. Usage Cited in: Biomark Res. 2025 Apr 7;13(1):57. [Abstract]

Flow cytometry histogram showing the level of aggresome in MM cell lines ARP1 and U266 after treatment with DMSO control and I3MO (10 μM) and Tubacin (10 μM) for 6 h. Bar plots showing the mean fluorescence intensity (MFI) in corresponding cell lines and conditions.
Cell Death Dis

Alternative splicing of BAZ1A in colorectal cancer disrupts the DNA damage response and increases chemosensitization. [Abstract]

2024 Aug 7;15(8):570. PMID: 39112459
Cell Commun Signal

Tubacin alleviate the reproductive toxicity of deoxynivalenol in mouse oocytes and zygotes via strengthening microtubule stability. [Abstract]

2025 Oct 3;23(1):417. PMID: 41044645

Tubacin purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Oct 3;23(1):417. [Abstract]

Representative light microscopy images showing oocytes in control, DON, and DON + Tubacin (2-6 μM) oocytes. Scale bar, 200 μm. Quantification of the percentage of oocytes that undergo PBE in vitro.

Tubacin purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Oct 3;23(1):417. [Abstract]

Representative light microscopy images showing oocytes in control, DON, and DON + Tubacin (4 μM) oocytes. Scale bar, 200 μm. C Quantification of the percentage of oocytes that undergo PBE in vitro.

Tubacin purchased from MedChemExpress. Usage Cited in: Cell Commun Signal. 2025 Oct 3;23(1):417. [Abstract]

Representative immunofluorescence images demonstrating spindle assembly in mouse metaphase I oocytes from control, DON, and DON + Tubacin (4 μM). Green, microtubule (β-Tubulin); red, aMTOCs (γ-Tubulin); blue, DNA (DAPI). Scale bar, 10 μm.
Apoptosis

Valproic acid upregulates the expression of the p75NTR/sortilin receptor complex to induce neuronal apoptosis. [Abstract]

2020 Oct;25(9-10):697-714. PMID: 32712736
Genes Dev

The microtubule regulator ringer functions downstream from the RNA repair/splicing pathway to promote axon regeneration. [Abstract]

2020 Feb 1;34(3-4):194-208. PMID: 31919191
Biomed Pharmacother

Histone deacetylase 6 negatively regulated microRNA-199a-5p induces the occurrence of preeclampsia by targeting VEGFA in vitro. [Abstract]

2019 Jun:114:108805. PMID: 30947018

Tubacin purchased from MedChemExpress. Usage Cited in: Biomed Pharmacother. 2019 Jun:114:108805. [Abstract]

Western blot is used for the detection of VEGFA and Hsp-90 with or without the treatment of Tubacin.
Cell Death Discov

Mutant p53 upregulates HDAC6 to resist ER stress and facilitates Ku70 deacetylation, which prevents its degradation and mitigates DNA damage in colon cancer cells. [Abstract]

2025 Apr 10;11(1):162. PMID: 40210861
Clin Transl Med

Pathogenic variants of TUBB8 cause oocyte spindle defects by disrupting with EB1/CAKP5 interactions and potential treatment targeting microtubule acetylation through HDAC6 inhibition. [Abstract]

2025 Jan;15(1):e70193. PMID: 39834092
EMBO Rep

HDAC6 deacetylates ENKD1 to regulate mitotic spindle behavior and corneal epithelial homeostasis. [Abstract]

2025 Mar 28. PMID: 40155750
JCI Insight

Transcriptional control of a collagen deposition and adhesion process that promotes lung adenocarcinoma growth and metastasis. [Abstract]

2022 Jan 11;7(1):e153948. PMID: 34874914
Int J Oncol

Epigenetic inhibitors eliminate senescent melanoma BRAFV600E cells that survive long‑term BRAF inhibition. [Abstract]

2020 Jun;56(6):1429-1441. PMID: 32236593
J Cell Physiol

Inhibition of HDAC6 by tubastatin A disrupts mouse oocyte meiosis via regulating histone modifications and mRNA expression. [Abstract]

2020 Oct;235(10):7030-7042. PMID: 32017059
Sci Rep

Transcription factor 4 promotes increased corneal endothelial cellular migration by altering microtubules in Fuchs endothelial corneal dystrophy. [Abstract]

2024 May 4;14(1):10276. PMID: 38704483
J Pharmacol Exp Ther

Downregulation of TrkB Expression and Signaling by Valproic Acid and Other Histone Deacetylase Inhibitors. [Abstract]

2019 Sep;370(3):490-503. PMID: 31308194
Biochim Biophys Acta Mol Cell Res

The yin and yang interplay between HDAC6 and KDM1 regulates the lysosomal degradation of mutp53 in colon cancer cells undergoing TG-induced ER stress. [Abstract]

2026 Jun;1873(5):120164. PMID: 42203131
FEBS Lett

Septin 9 PB domains coordinate centrosome positioning and microtubule acetylation to control epithelial polarity. [Abstract]

2026 May 26. PMID: 42186334
Research Square Print

2023 Mar 9.
Università degli Studi di CAGLIARI

2021 May.
Florida Southern College

2019 May.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (138.53 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3853 mL

6.9266 mL

13.8531 mL

5 mM

0.2771 mL

1.3853 mL

2.7706 mL

10 mM

0.1385 mL

0.6927 mL

1.3853 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protokoll

Cell Assay^[3]

HDAC inhibitors TSA, VPA, tubacin, and TBSA are used in the assay. Cytotoxicity of HDACi to TE671 and BHK-21 cells is evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. 5 × 10⁴ cells per well are seeded in 96-well plates and then treated with the indicated concentration of each HDACi. After 48-h of treatment, 25 μL of MTT solution (5 mg/mL) is added to each well and incubated at 37 °C with 5% CO2 for 3 h. After three washings with phosphate buffer saline (PBS), 100 μL DMSO is added into each well for dissolving formazan crystals. OD570−630 is measured by micro-ELISA reader and survival rate are calculated to indicate suppressive effects of each HDACi on the survival of TE671 and BHK-21 cells. Survival rate (%) = ((Acontrol − Aexperiment)/Acontrol) × 100%. 50% cytotoxic concentration (CC₅₀) values are calculated by computer program^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Butler KV, et al. Rational design and simple chemistry yield a superior, neuroprotective HDAC6 inhibitor, tubastatin A. J Am Chem Soc. 2010 Aug 11;132(31):10842-6. [Content Brief]

[2]. Hideshima T, et al. Small-molecule inhibition of proteasome and aggresome function induces synergistic antitumor activity in multiple myeloma. Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8567-72. Epub 2005 Jun 3. [Content Brief]

[3]. Lu CY, et al. Tubacin, an HDAC6 Selective Inhibitor, Reduces the Replication of the Japanese Encephalitis Virus via the Decrease of Viral RNA Synthesis. Int J Mol Sci. 2017 May 1;18(5). [Content Brief]

[4]. Severin Lechner, et al. Target deconvolution of HDAC pharmacopoeia reveals MBLAC2 as common off-target. Nat Chem Biol. 2022 Apr 28. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3853 mL	6.9266 mL	13.8531 mL	34.6328 mL
	5 mM	0.2771 mL	1.3853 mL	2.7706 mL	6.9266 mL
	10 mM	0.1385 mL	0.6927 mL	1.3853 mL	3.4633 mL
	15 mM	0.0924 mL	0.4618 mL	0.9235 mL	2.3089 mL
	20 mM	0.0693 mL	0.3463 mL	0.6927 mL	1.7316 mL
	25 mM	0.0554 mL	0.2771 mL	0.5541 mL	1.3853 mL
	30 mM	0.0462 mL	0.2309 mL	0.4618 mL	1.1544 mL
	40 mM	0.0346 mL	0.1732 mL	0.3463 mL	0.8658 mL
	50 mM	0.0277 mL	0.1385 mL	0.2771 mL	0.6927 mL
	60 mM	0.0231 mL	0.1154 mL	0.2309 mL	0.5772 mL
	80 mM	0.0173 mL	0.0866 mL	0.1732 mL	0.4329 mL
	100 mM	0.0139 mL	0.0693 mL	0.1385 mL	0.3463 mL

Keywords

Tubacin

25 Cited Publications

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Tubacin

Anti-Infection Compound Library

Cell Cycle/DNA Damage Compound Library

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Lactylation Compound Library

Publications Citing Use of MedChemExpress (MCE) Tubacin

Alle Beta-lactamase Isoform-spezifische Produkte anzeigen

Alle HDAC Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (25)

Lösungsmittel & Löslichkeit

Protokoll

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Tubacin Related Classifications

HY-13428 Related Classifications