Cat. No.: HY-N6972

Cepharanthine

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Based on 14 publication(s) in Google Scholar

Cepharanthine is a natural product that can be isolated from the plant Stephania cephalantha Hayata. Cepharanthine has anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) activities. Cepharanthine has good effective in suppressing viral proliferation (half maximal (50%) inhibitory concentration (IC₅₀) and 90% inhibitory concentration (IC₉₀) values of 1.90 and 4.46 μM. Cepharanthine can also effectively reverses P-gp-mediated multidrug resistance in K562 cells and increase enhances the sensitivity of anticancer agents in xenograft mice model. Cepharanthine shows inhibitory effects of human liver cytochrome P450 enzymes CYP3A4, CYP2E1 and CYP2C9. Cepharanthine has antitumor, anti-inflammatory and antinociceptive effects.

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Pureté: 99.87%
CAS No.: 481-49-2
Formule: C₃₇H₃₈N₂O₆
Masse moléculaire:606.71

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Cepharanthine Chemical Structure

CAS No.: 481-49-2

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
- Essai clinique
Chemical Information
Publications (14)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Cepharanthine is a natural product that can be isolated from the plant Stephania cephalantha Hayata. Cepharanthine has anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) activities. Cepharanthine has good effective in suppressing viral proliferation (half maximal (50%) inhibitory concentration (IC₅₀) and 90% inhibitory concentration (IC₉₀) values of 1.90 and 4.46 μM^[1]. Cepharanthine can also effectively reverses P-gp-mediated multidrug resistance in K562 cells and increase enhances the sensitivity of anticancer agents in xenograft mice model^[2]^[3]. Cepharanthine shows inhibitory effects of human liver cytochrome P450 enzymes CYP3A4, CYP2E1 and CYP2C9. Cepharanthine has antitumor, anti-inflammatory and antinociceptive effects^[4]^[5]^[6]^[7]^[8].

IC₅₀ & Target^[4]

CYP3A4

16.29 μM (IC₅₀)

CYP2E1

25.62 μM (IC₅₀)

CYP2C9

24.57 μM (IC₅₀)

Voir tous les produits spécifiques à Isoform Cytochrome P450 :

Voir tous les produits spécifiques à Isoform Parasite :

Cellular Effect

Cell Line	Type	Value	Description	References
A-431	ED₅₀	2.1 μg/mL Compound: 11	Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay Cytotoxicity against human A431 cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
A549	IC₅₀	5 μM Compound: 7	Cytotoxicity against human A549 cells after 48 hrs by MTS assay Cytotoxicity against human A549 cells after 48 hrs by MTS assay	[PMID: 23621840]
A673	GI₅₀	4.5 μM Compound: Cepheranthine	Antiproliferative activity against human A673 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human A673 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
ECa-109 cell line	IC₅₀	>10 μM Compound: 7	Cytotoxicity against human ECA109 cells after 48 hrs by MTT assay Cytotoxicity against human ECA109 cells after 48 hrs by MTT assay	[PMID: 23621840]
ECa-109 cell line	IC₅₀	436.7 nM Compound: CEP	Reversal of VCR -resistance in human Eca-109 cells assessed as cell viability at 5 uM incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM) Reversal of VCR -resistance in human Eca-109 cells assessed as cell viability at 5 uM incubated for 48 hrs by CCK-8 method (Rvb = 6830.0+/-537.0 nM)	[PMID: 36892076]
HCC1806	GI₅₀	7.2 μM Compound: Cepheranthine	Antiproliferative activity against human HCC1806 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC1806 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HCC1937	GI₅₀	6 μM Compound: Cepheranthine	Antiproliferative activity against human HCC1937 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC1937 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HCC70	GI₅₀	5.8 μM Compound: Cepheranthine	Antiproliferative activity against human HCC70 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human HCC70 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
HEK-293T	CC₅₀	2.1 μM Compound: 3; cep	Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay Cytotoxicity against HEK293T cells assessed as reduction in cell viability by CellTiter-Glo assay	[PMID: 37043739]
HeLa	IC₅₀	1.53 μM Compound: Cepharanthine	Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay Inhibition of Ebolavirus glycoprotein/matrix protein VP40 entry in human HeLa cells after 4.5 hrs beta-lactamase reporter assay	[PMID: 29624387]
HL-60	IC₅₀	9.2 μM Compound: 7	Cytotoxicity against human HL60 cells after 48 hrs by MTS assay Cytotoxicity against human HL60 cells after 48 hrs by MTS assay	[PMID: 23621840]
HT-1080	ED₅₀	6.1 μg/mL Compound: 11	Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay Cytotoxicity against human HT1080 cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
KB	ED₅₀	5.9 μg/mL Compound: 11	Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay Cytotoxicity against human KB cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
KB	ED₅₀	9700 nM Compound: 12	Cytotoxicity against human KB cells after 72 hrs by SRB assay Cytotoxicity against human KB cells after 72 hrs by SRB assay	[PMID: 9917283]
KB-V1	ED₅₀	0.9 μg/mL Compound: 11	Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human KBV1 cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
LNCaP	ED₅₀	5.6 μg/mL Compound: 11	Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay Cytotoxicity against human LNCAP cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
MCF7	IC₅₀	2.9 μM Compound: 7	Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay	[PMID: 23621840]
MDA-MB-231	GI₅₀	5.3 μM Compound: Cepheranthine	Antiproliferative activity against human MDA-MB-231 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human MDA-MB-231 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
MDA-MB-453	GI₅₀	5.5 μM Compound: Cepheranthine	Antiproliferative activity against human MDA-MB-453 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human MDA-MB-453 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
MOLT-4	CC₅₀	10 μg/mL Compound: CEP, Cepharanthine	Cytotoxicity against human MOLT4 cells assessed as cell growth inhibition after 24 hrs by MTT assay Cytotoxicity against human MOLT4 cells assessed as cell growth inhibition after 24 hrs by MTT assay	[PMID: 24704028]
P388	ED₅₀	0.3 μg/mL Compound: 11	Cytotoxicity against mouse P388 cells after 2 days by sulforhodamine B assay Cytotoxicity against mouse P388 cells after 2 days by sulforhodamine B assay	[PMID: 8450319]
SJRH30	GI₅₀	3.8 μM Compound: Cepheranthine	Antiproliferative activity against human SJRH30 assessed as cell growth inhibition after 48 hrs by SRB assay Antiproliferative activity against human SJRH30 assessed as cell growth inhibition after 48 hrs by SRB assay	[PMID: 33226219]
SK-MEL-2	ED₅₀	14.5 μg/mL Compound: 11	Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay Cytotoxicity against human SK-MEL-2 cells after 3 days by sulforhodamine B assay	[PMID: 8450319]
SMMC-7721	IC₅₀	9.9 μM Compound: 7	Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay	[PMID: 23621840]
SW480	IC₅₀	4.7 μM Compound: 7	Cytotoxicity against human SW480 cells after 48 hrs by MTS assay Cytotoxicity against human SW480 cells after 48 hrs by MTS assay	[PMID: 23621840]
U-937	IC₅₀	>50 μM Compound: 8, NSC 623442	Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay Cytotoxicity against human U937 cells after 24 hrs by alamar blue assay	[PMID: 22766217]
Vero	CC₅₀	>50 μM Compound: Cepharanthine	Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr Cell viability measured by CellTiter-Glo assay in Vero cells at MOI 0.05 after 72hr	10.1101/2020.03.20.999730
Vero	IC₅₀	4.47 μM Compound: Cepharanthine	Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr Antiviral activity against SARS-CoV-2 (viral titer) measured by plaque assay in Vero cells at MOI 0.0125 after 24 hr	10.1101/2020.03.20.999730
Vero C1008	EC₅₀	0.98 μM Compound: Cepharanthine	Antiviral activity against SARS-Co-V-2 infected in Vero E6 cells assessed as reduction in viral replication Antiviral activity against SARS-Co-V-2 infected in Vero E6 cells assessed as reduction in viral replication	[PMID: 37229831]
ZR-75-1	ED₅₀	1.2 μg/mL Compound: 11	Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay Cytotoxicity against human ZR-75-1 cells after 3 days by sulforhodamine B assay	[PMID: 8450319]

In Vitro

Cepharanthine (CEP) (2 μM, 48 h) inhibits cell viability and colony formation and induces apoptosis via the mitochondrial pathway in human TNBC cells^[2].
Cepharanthine (2 μM, 48 h) Combinates with Epirubicin (HY-13624) impairs mitochondrial function and causes mitochondrial fission and apoptosis in MDA-MB-231 cells^[2].
Cepharanthine (5 μM, 24 h) potently enhances the sensitivity of anticancer agents Doxorubicin (HY-15142A) and Vincristine (HY-N0488) and enhanced apoptosis induced by anticancer agents in K562 cells^[3].
Cepharanthine (10-50 μM, 0.5-1 h) changes the distribution of Doxorubicin (HY-15142A) from cytoplasmic vesicles to nucleoplasm in K562 cells by inhibiting the acidification of cytoplasmic organellesin^[3].
Cepharanthine (0-50 μM, 30 min) shows inhibitory effects of human liver cytochrome P450 enzymes CYP3A4,CYP2E1 and CYP2C9in vitro^[4].
Cepharanthine(0-4 μM, 48 hours) blocks P. falciparum development in ring stage with IC₅₀s of 3.059, 0.927, 2.276, and 1.803 μM for FCM29, W2, 3D7 and K1, respectively^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[2]

Cell Line:

MDAMB-231 and BT549 cells
Concentration:

2 μM
Incubation Time:

48 h
Result:

Cepharanthine alone minimally increased apoptosis (~5% to ~10%), whereas combinated with Epirubicin (HY-13624) markedly increased apoptosis (~50%).

Western Blot Analysis^[2]

Cell Line:

MDAMB-231 cells
Concentration:

2 μM
Incubation Time:

48 h
Result:

Combinated with Epirubicin (HY-13624) markedly resulted in oxidation of the actin-remodeling protein cofilin, which promoted formation of an intramolecular disulfide bridge between Cys39, Cys80 and Ser3 dephosphorylation, leading to mitochondria translocation of cofilin.
Combinated with Epirubicin (HY-13624) induced mitochondrial fission in MDA-MB-231 cells.

Immunofluorescence^[3]

Cell Line:

K562 cells or MIA-PaCa-2 cells
Concentration:

10,20,25,50 μM
Incubation Time:

0.5 h or 1 h
Result:

Made the intracellular localization of Doxorubicin (HY-15142A) in cytoplasmic vesicles shifted to the nucleoplasm.
Decreased red AO (weakly basic fluorescence probe) fluorescence by dose-dependent mannar in K562 cells.

Cell Viability Assay^[5]

Cell Line:

P. falciparum cultivated in type A+ human erythrocytes
Concentration:

2 μM
Incubation Time:

48 h
Result:

Blocked P. falciparum development in ring stage with IC₅₀s of 3.059, 0.927, 2.276, and 1.803 μM for FCM29, W2, 3D7 and K1, respectively.

In Vivo

Cepharanthine (12 mg/kg, i.p., once daily for 36 days) enhances the therapeutic efficacy of Epirubicin (HY-13624) in MDA-MB-231 cell xenografts^[2].
Cepharanthine (10 mg/kg, i.p., single dose) prevents LPS-induced pulmonary vascular injury in rats by inhibiting leukocyte activation^[6].
Cepharanthine (CE)(10 mg/kg, i.p., single dose) exerts anti-inflammatory effects via NF-kB inhibition in a LPS-induced rat model of systemic inflammation^[7].
Cepharanthine (20-180 mg/kg, i.p.) results in a dose-dependent antinociceptive effect with an ED₅₀ value of 24.5 mg/kg in mice pain models^[8].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

MDA-MB-231 cell xenografts in mice^[1]
Dosage:

12 mg/kg
Administration:

Intraperitoneal injection (i.p.), once daily for 36 days
Result:

Combinated with Epirubicin (HY-13624) greatly enhanced the therapeutic efficacy compared with administration of either drug alone.

Animal Model:

LPS-induced pulmonary vascular injury in male Wistar rats^[6]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose
Result:

Decreased LPS-induced pulmonary vascular injury.
Significantly inhibited the increases in plasma tumor necrosis factor-a (TNF-a) concentrations.

Animal Model:

LPS-induced Wistar rat model of systemic inflammation^[7]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.), single dose
Result:

Significantly inhibited the increase in LPS-induced IL-6, TNF-α and nitrate/nitrite levels.
Reduced interstitial edema and inflammatory cell compared with the control group.
Reduced pathologic abnormalities, such as vacuolization, dot necrosis, striped necrosis, and bridging necrosis appeared, and inflammatory cells compared with the control group.
group compared with the LPS group.

Animal Model:

Mice pain models in Kunming (KM) strain male and female mice ^[8]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection (i.p.)
Result:

Resulted in a dose-dependent antinociceptive effect with an ED₅₀ value of 24.5 mg/kg in mice pain models.
Significantly decreased the intestinal propulsion with maximal inhibition at 33.6%.

Essai clinique

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS No. 481-49-2
Appearance Solid
Masse moléculaire 606.71
Formule C₃₇H₃₈N₂O₆
Color White to yellow
SMILES

[H][C@]12N(C)CCC(C1=C3)=CC(OC)=C3OC4=C5C(OCO5)=CC6=C4[C@](N(C)CC6)([H])CC(C=C7)=CC=C7OC8=C(OC)C=CC(C2)=C8
Structure Classification
- Alkaloids
- Isoquinoline Alkaloids
Initial Source
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (14)

All WBCell Proliferation/Viability AssayIFBio/Physico-chemical AssayFlow Cytometry

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Journal Impact Factor

J Transl Med

Single-cell profiling of SLC family transporters: uncovering the role of SLC7A1 in osteosarcoma. [Abstract]

2025 Jan 22;23(1):103. PMID: 39844299

Cepharanthine purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 22;23(1):103. [Abstract]

Cepharanthine (CPE) (2–5 μM) dose-dependently inhibited the proliferation of 143B cells.

Cepharanthine purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 22;23(1):103. [Abstract]

Cepharanthine (CPE) (2–5 μM) enhanced its inhibitory effects on arginine uptake in 143B cells with increasing concentration.

Cepharanthine purchased from MedChemExpress. Usage Cited in: J Transl Med. 2025 Jan 22;23(1):103. [Abstract]

Cepharanthine (CPE) (2–5 μM; 48 h) pretreatment significantly downregulated CD206 expression and upregulated CD86 expression in THP1-derived macrophages in a dose-dependent manner.
Am J Chin Med

Cepharanthine Triggers Ferroptosis in Gastric Cancer by PINK1/FUNDC1-Mediated Mitophagy-Dependent GPX4 Degradation. [Abstract]

2026;54(1):329-348. PMID: 41684093
Int Immunopharmacol

Cepharanthine inhibits NLRP3 inflammasome-induced pyroptosis through ARIH2-mediated degradation of NLRP3. [Abstract]

2026 Sep 1:184:116935. PMID: 42217341
Virol J

Bisbenzylisoquinoline alkaloids inhibit influenza virus replication by disrupting endosomal acidification. [Abstract]

2025 Jun 4;22(1):181. PMID: 40468427

Cepharanthine purchased from MedChemExpress. Usage Cited in: Virol J. 2025 Jun 4;22(1):181. [Abstract]

Cepharanthine (CPE) (0.33-3 nM; 24 h) dose-dependently suppressed protein levels of the viral PB2 in A549 cells.

Cepharanthine purchased from MedChemExpress. Usage Cited in: Virol J. 2025 Jun 4;22(1):181. [Abstract]

Cepharanthine (CPE) (0.33-3 nM; 24 h) significantly reduced the expression of the IAV M2 protein in MDCK cells.
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607
Vet Microbiol

Antiviral activity of Stephania japonica extract against porcine epidemic diarrhea virus infection. [Abstract]

2025 Sep:308:110647. PMID: 40706130
Vet Microbiol

Cepharanthine mitigates NIBV-induced pyroptosis via the MDA5/NF-κB/NLRP3 signaling pathway. [Abstract]

2025 Apr 18:305:110519. PMID: 40273551
Virology

Cepharanthine: A promising natural compound against feline infectious peritonitis virus infection and associated inflammation. [Abstract]

2025 Jan 23:604:110422. PMID: 39884162
Arch Dermatol Res

Cepharantine prevents hypertrophic scarring by regulating the TGF-β/SMAD pathway. [Abstract]

2025 Apr 24;317(1):734. PMID: 40274641
Am J Transl Res

Cepharanthine inhibits migration, invasion, and EMT of bladder cancer cells by activating the Rap1 signaling pathway in vitro. [Abstract]

2024 May 15;16(5):1602-1619. PMID: 38883391
SSRN

2026 Mar 15.
SSRN

2023 Sep 21.
Oxid Med Cell Longev

Cepharanthine Attenuates Early Brain Injury after Subarachnoid Hemorrhage in Mice via Inhibiting 15-Lipoxygenase-1-Mediated Microglia and Endothelial Cell Ferroptosis. [Abstract]

2022 Feb 9;2022:4295208. PMID: 35186185
bioRxiv

2020 Jun.

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (82.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6482 mL

8.2412 mL

16.4823 mL

5 mM

0.3296 mL

1.6482 mL

3.2965 mL

10 mM

0.1648 mL

0.8241 mL

1.6482 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: 2.5 mg/mL (4.12 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 2

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (3.43 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 6.02 mg/mL (9.92 mM); Suspended solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 15% Cremophor EL 85% Saline

Solubility: 6.02 mg/mL (9.92 mM); Suspended solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

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Références

[1]. Hijikata A, et al. Evaluating cepharanthine analogues as natural drugs against SARS-CoV-2. FEBS Open Bio. 2022;12(1):285-294 [Content Brief]

[2]. Shen LW, et.al. Cepharanthine sensitizes human triple negative breast cancer cells to chemotherapeutic agent epirubicin via inducing cofilin oxidation-mediated mitochondrial fission and apoptosis. Acta Pharmacol Sin. 2022 Jan;43(1):177-193. [Content Brief]

[3]. Ikeda R, et.al. Cepharanthine potently enhances the sensitivity of anticancer agents in K562 cells. Cancer Sci. 2005 Jun;96(6):372-6. [Content Brief]

[4]. Zhang X, et.al. In vitro inhibitory effects of cepharanthine on human liver cytochrome P450 enzymes. Pharm Biol. 2020 Dec;58(1):247-252. [Content Brief]

[5]. Hua P, et.al. Cepharanthine induces apoptosis through reactive oxygen species and mitochondrial dysfunction in human non-small-cell lung cancer cells. Biochem Biophys Res Commun. 2015 May 1;460(2):136-42. [Content Brief]

[6]. Desgrouas C, et.al. In vitro antiplasmodial activity of cepharanthine. Malar J. 2014 Aug 22;13:327. [Content Brief]

[7]. Murakami K, et.al. The prevention of lipopolysaccharide-induced pulmonary vascular injury by pretreatment with cepharanthine in rats. Am J Respir Crit Care Med. 2000 Jan;161(1):57-63. [Content Brief]

[8]. Wei XY, et,al. Antinociceptive activities and mechanism of action of Cepharanthine. Biochem Biophys Res Commun. 2022 Jul 23;614:219-224. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6482 mL	8.2412 mL	16.4823 mL	41.2058 mL
	5 mM	0.3296 mL	1.6482 mL	3.2965 mL	8.2412 mL
	10 mM	0.1648 mL	0.8241 mL	1.6482 mL	4.1206 mL
	15 mM	0.1099 mL	0.5494 mL	1.0988 mL	2.7471 mL
	20 mM	0.0824 mL	0.4121 mL	0.8241 mL	2.0603 mL
	25 mM	0.0659 mL	0.3296 mL	0.6593 mL	1.6482 mL
	30 mM	0.0549 mL	0.2747 mL	0.5494 mL	1.3735 mL
	40 mM	0.0412 mL	0.2060 mL	0.4121 mL	1.0301 mL
	50 mM	0.0330 mL	0.1648 mL	0.3296 mL	0.8241 mL
	60 mM	0.0275 mL	0.1374 mL	0.2747 mL	0.6868 mL
	80 mM	0.0206 mL	0.1030 mL	0.2060 mL	0.5151 mL

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Cepharanthine

14 Cited Publications

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