Cat. No.: HY-N1921

Edpetiline

2 Cited Publications

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Based on 2 publication(s) in Google Scholar

Edpetiline is an anti-inflammatory agent. Edpetiline inhibits the phosphorylation of IκB, nuclear transcription/translocation of NF-κB p65, as well as the phosphorylation of p38 MAPK and ERK MAPK. Edpetiline reduces intracellular ROS levels, inhibits the expression of TNF-α, IL-6, iNOS, COX-2, and promotes the expression of IL-4. Edpetiline is applicable to the research of diseases associated with inflammation and oxidative stress.

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Pureté: 99.81%
CAS No.: 32685-93-1
Formule: C₃₃H₅₃NO₈
Masse moléculaire:591.78

Stockage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Edpetiline Chemical Structure

CAS No.: 32685-93-1

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

COX-2

TNF-α

IL-6

IL-4

Voir tous les produits spécifiques à Isoform NF-κB :

Voir tous les produits spécifiques à Isoform p38 MAPK :

Voir tous les produits spécifiques à Isoform TNF Receptor :

Voir tous les produits spécifiques à Isoform COX :

Voir tous les produits spécifiques à Isoform Interleukin Related :

In Vitro

Edpetiline (0-200 μM) exhibits no cytotoxicity against RAW264.7 cells at concentrations up to 100 μM, but induces significant cytotoxicity at 200 μM^[1].
Edpetiline (0-100 μM; 1 h) inhibits LPS (HY-D1056)-induced production of TNF-α and IL-6 in RAW264.7 macrophages by suppressing the transcription of TNF-α and IL-6 mRNA, and promotes the production of the IL-4 cytokine^[1].
Edpetiline (0-100 μM; 1 h) inhibits LPS-induced expression of iNOS and COX-2 at both protein and mRNA levels, and suppresses ROS production in RAW264.7 macrophages^[1].
Edpetiline (0-100 μM; 1 h) inhibits the activation of p38 and ERK MAPK signaling pathways in LPS-induced RAW264.7 macrophages, and suppresses the NF-κB signaling pathway by blocking IκB-α degradation and NF-κB p65 nuclear translocation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

0, 25, 100 μM
Incubation Time:

1 h
Result:

Suppressed LPS-induced TNF-α and IL-6 production.
Increased the expression of IL-4 mRNA, but had an insignificant effect on the expression level of IL-10 mRNA.
Suppressed the up-regulation of iNOS and COX-2 mRNA expression induced by LPS.

ELISA Assay^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

0, 25, 50, 100 μM
Incubation Time:

1 h
Result:

Suppressed LPS-induced TNF-α and IL-6 mRNA levels.

Western Blot Analysis^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

25, 50, 100 μM
Incubation Time:

1 h
Result:

Reduced the expressions of iNOS and COX-2 proteins.
Inhibited the expressions of P-p38, P-ERK, proteins.
Inhibited the decrease of IκB-α and the expression of NF-κB p65 in the nucleus at 50 or 100 μM.

Chemical Information

CAS No. 32685-93-1
Appearance Solid
Masse moléculaire 591.78
Formule C₃₃H₅₃NO₈
Color White to off-white
SMILES

C[C@@]([C@@]1([H])C2=O)(CC[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)C1)[C@]4([H])[C@](C2)([H])[C@@](CC[C@@]5([H])[C@@]6([H])CN(C[C@@H](C)CC7)[C@]7([H])[C@]5(O)C)([H])[C@@]6([H])C4
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

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Phytomedicine

Total alkaloids of Fritillaria unibracteata var. wabuensis bulbus ameliorate chronic asthma via the TRPV1/Ca2+/NFAT pathway. [Abstract]

2023 Sep:118:154946. PMID: 37421766
Molecules

Metabolite Profiling and Anti-Inflammatory Activities of Fritillaria cirrhosa D. Don Bulbs Derived from Tissue Culture. [Abstract]

2025 Jan 31;30(3):623. PMID: 39942727

Solvant et solubilité

In Vitro:

DMSO : 33.33 mg/mL (56.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6898 mL

8.4491 mL

16.8982 mL

5 mM

0.3380 mL

1.6898 mL

3.3796 mL

10 mM

0.1690 mL

0.8449 mL

1.6898 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (282 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhang H, et al. Effects of edpetiline from Fritillaria on inflammation and oxidative stress induced by LPS stimulation in RAW264.7 macrophages. Acta Biochim Biophys Sin (Shanghai). 2021;53(2):229-237. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6898 mL	8.4491 mL	16.8982 mL	42.2454 mL
	5 mM	0.3380 mL	1.6898 mL	3.3796 mL	8.4491 mL
	10 mM	0.1690 mL	0.8449 mL	1.6898 mL	4.2245 mL
	15 mM	0.1127 mL	0.5633 mL	1.1265 mL	2.8164 mL
	20 mM	0.0845 mL	0.4225 mL	0.8449 mL	2.1123 mL
	25 mM	0.0676 mL	0.3380 mL	0.6759 mL	1.6898 mL
	30 mM	0.0563 mL	0.2816 mL	0.5633 mL	1.4082 mL
	40 mM	0.0422 mL	0.2112 mL	0.4225 mL	1.0561 mL
	50 mM	0.0338 mL	0.1690 mL	0.3380 mL	0.8449 mL

Keywords

Edpetiline

2 Cited Publications

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Edpetiline

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Publications Citing Use of MedChemExpress (MCE) Edpetiline

Voir tous les produits spécifiques à Isoform NF-κB

Voir tous les produits spécifiques à Isoform p38 MAPK

Voir tous les produits spécifiques à Isoform TNF Receptor

Voir tous les produits spécifiques à Isoform COX

Voir tous les produits spécifiques à Isoform Interleukin Related

Activité biologique

Chemical Information

Publications (2)

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Edpetiline Related Classifications

HY-N1921 Related Classifications