Cat. No.: HY-N1921

Edpetiline

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Edpetiline is an anti-inflammatory agent. Edpetiline inhibits the phosphorylation of IκB, nuclear transcription/translocation of NF-κB p65, as well as the phosphorylation of p38 MAPK and ERK MAPK. Edpetiline reduces intracellular ROS levels, inhibits the expression of TNF-α, IL-6, iNOS, COX-2, and promotes the expression of IL-4. Edpetiline is applicable to the research of diseases associated with inflammation and oxidative stress.

For research use only. We do not sell to patients.

Purity: 99.81%
CAS No.: 32685-93-1
Formula: C₃₃H₅₃NO₈
Molecular Weight:591.78

Storage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Edpetiline Chemical Structure

CAS No.: 32685-93-1

Size

Price

Stock

Quantity

Amount: USD 0.00

* Please select Quantity before adding items.

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[1]

COX-2

TNF-α

IL-6

IL-4

All NF-κB Isoforms :

All p38 MAPK Isoforms :

All TNF Receptor Isoforms :

All COX Isoforms :

All Interleukin Related Isoforms :

In Vitro

Edpetiline (0-200 μM) exhibits no cytotoxicity against RAW264.7 cells at concentrations up to 100 μM, but induces significant cytotoxicity at 200 μM^[1].
Edpetiline (0-100 μM; 1 h) inhibits LPS (HY-D1056)-induced production of TNF-α and IL-6 in RAW264.7 macrophages by suppressing the transcription of TNF-α and IL-6 mRNA, and promotes the production of the IL-4 cytokine^[1].
Edpetiline (0-100 μM; 1 h) inhibits LPS-induced expression of iNOS and COX-2 at both protein and mRNA levels, and suppresses ROS production in RAW264.7 macrophages^[1].
Edpetiline (0-100 μM; 1 h) inhibits the activation of p38 and ERK MAPK signaling pathways in LPS-induced RAW264.7 macrophages, and suppresses the NF-κB signaling pathway by blocking IκB-α degradation and NF-κB p65 nuclear translocation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

0, 25, 100 μM
Incubation Time:

1 h
Result:

Suppressed LPS-induced TNF-α and IL-6 production.
Increased the expression of IL-4 mRNA, but had an insignificant effect on the expression level of IL-10 mRNA.
Suppressed the up-regulation of iNOS and COX-2 mRNA expression induced by LPS.

ELISA Assay^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

0, 25, 50, 100 μM
Incubation Time:

1 h
Result:

Suppressed LPS-induced TNF-α and IL-6 mRNA levels.

Western Blot Analysis^[1]

Cell Line:

LPS-induced RAW264.7 cells
Concentration:

25, 50, 100 μM
Incubation Time:

1 h
Result:

Reduced the expressions of iNOS and COX-2 proteins.
Inhibited the expressions of P-p38, P-ERK, proteins.
Inhibited the decrease of IκB-α and the expression of NF-κB p65 in the nucleus at 50 or 100 μM.

Chemical Information

CAS No. 32685-93-1
Appearance Solid
Molecular Weight 591.78
Formula C₃₃H₅₃NO₈
Color White to off-white
SMILES

C[C@@]([C@@]1([H])C2=O)(CC[C@H](O[C@]([C@@H]([C@@H](O)[C@@H]3O)O)([H])O[C@@H]3CO)C1)[C@]4([H])[C@](C2)([H])[C@@](CC[C@@]5([H])[C@@]6([H])CN(C[C@@H](C)CC7)[C@]7([H])[C@]5(O)C)([H])[C@@]6([H])C4
Structure Classification
- Terpenoids
- Triterpenes
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Publications (2)

All

Sort by:

Journal Impact Factor

Phytomedicine

Total alkaloids of Fritillaria unibracteata var. wabuensis bulbus ameliorate chronic asthma via the TRPV1/Ca2+/NFAT pathway. [Abstract]

2023 Sep:118:154946. PMID: 37421766
Molecules

Metabolite Profiling and Anti-Inflammatory Activities of Fritillaria cirrhosa D. Don Bulbs Derived from Tissue Culture. [Abstract]

2025 Jan 31;30(3):623. PMID: 39942727

Solvent & Solubility

In Vitro:

DMSO : 33.33 mg/mL (56.32 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6898 mL

8.4491 mL

16.8982 mL

5 mM

0.3380 mL

1.6898 mL

3.3796 mL

10 mM

0.1690 mL

0.8449 mL

1.6898 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (4.22 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zhang H, et al. Effects of edpetiline from Fritillaria on inflammation and oxidative stress induced by LPS stimulation in RAW264.7 macrophages. Acta Biochim Biophys Sin (Shanghai). 2021;53(2):229-237. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6898 mL	8.4491 mL	16.8982 mL	42.2454 mL
	5 mM	0.3380 mL	1.6898 mL	3.3796 mL	8.4491 mL
	10 mM	0.1690 mL	0.8449 mL	1.6898 mL	4.2245 mL
	15 mM	0.1127 mL	0.5633 mL	1.1265 mL	2.8164 mL
	20 mM	0.0845 mL	0.4225 mL	0.8449 mL	2.1123 mL
	25 mM	0.0676 mL	0.3380 mL	0.6759 mL	1.6898 mL
	30 mM	0.0563 mL	0.2816 mL	0.5633 mL	1.4082 mL
	40 mM	0.0422 mL	0.2112 mL	0.4225 mL	1.0561 mL
	50 mM	0.0338 mL	0.1690 mL	0.3380 mL	0.8449 mL

Keywords

Product Name:
Cat. No.:
Quantity:
MCE Japan Authorized Agent:

Product Name:
CAS No.:
Species:
Purity:
Quantity:

Edpetiline

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Kinase Inhibitor Library

MAPK Compound Library

Metabolism/Protease Compound Library

NF-κB Signaling Compound Library

Stem Cell Signaling Compound Library

Natural Product Library

Anti-Aging Compound Library

Antioxidant Compound Library

Differentiation Inducing Compound Library

Reprogramming Compound Library

Glycoside Compound Library

Oxygen Sensing Compound Library

Terpenoids Library

Pyroptosis Compound Library

Anti-Breast Cancer Compound Library

Anti-Lung Cancer Compound Library

Anti-Pancreatic Cancer Compound Library

Anti-Blood Cancer Compound Library

Anti-Cancer Metabolism Compound Library

Anti-Parkinson's Disease Compound Library

Neurodegenerative Disease-related Compound Library

Anti-Obesity Compound Library

Angiogenesis-Related Compound Library

Transcription Factor-Targeted Library

Anti-Liver Cancer Compound Library

Anti-Colorectal Cancer Compound Library

Antidepressant Compound Library

Plant-Sourced Natural Product Library

Anti-Prostate Cancer Compound Library

Anti-Pulmonary Fibrosis Compound Library

Cancer Stem Cells Compound Library

Heterocyclic Compound Library

Pain-Related Compound Library

Mitochondrial Protection Compound Library

Membrane Protein-targeted Compound Library

Membrane Receptor-targeted Compound Library

Autoimmune Disease Compound Library

Cytokine Inhibitors Library

Serine/Threonine Kinase Inhibitor Library

Anti-Hematopathy Compound Library

Anti-Ovarian Cancer Compound Library

Multi-Target Compound Library

Radioprotector Library

Bioactive Compound Library Max

MCE Bioactive Compound Library

Natural Product Library Plus

Natural Product and Natural Product-Like Compound Library

Bioactive Compound Library

Anti-Gastric Cancer Compound Library

Anti-Fibrosis Compound Library

Protein Kinase Compound Library

Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library

Cell Proliferation Compound Library

High-Throughput Bioactive Compound Library

High-Throughput Natural Product Library

Anti-Rheumatic Arthritis Compound Library

Natural Product Diversity Scaffold Library

Lactylation Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Edpetiline

All NF-κB Isoforms

All p38 MAPK Isoforms

All TNF Receptor Isoforms

All COX Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Edpetiline Related Classifications

HY-N1921 Related Classifications