BRD6989
Based on 2 publication(s) in Google Scholar
BRD6989, an analog of the natural product cortistatin A (dCA), inhibits CDK8 and upregulates IL-10. BRD6989 selectively binds a complex of CDK8 with an IC50 of ~200 nM. BRD6989 inhibits the kinase activity of recombinant CDK8 or CDK19 complexes.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.54%
- No. CAS: 642008-81-9
- Fòrmula: C16H16N4
- Peso molecular:264.33
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) BRD6989
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Actividad biológica
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CDK8 ~200 nM (IC50) |
recombinant CDK8 ~0.5 μM (IC50) |
recombinant CDK19 >30 μM (IC50) |
IL-10 |
Pretreatment of BMDCs with BRD6989 (0-100 μM; for 48 hours) increases IL-10 production with an EC50 of ~1 μM[1].
BRD6989 (0.6, 1.7, 5, 15 μM) suppresses phosphorylation of the STAT1 transactivation domain at Ser727 in IFNγ-stimulated BMDCs[1].
BRD6989 (5 µM; ~2 hours) suppresses induction of STAT1–STAT2 activity and NF-κB activation to a varying degree after stimulation of BMDMs[1].
BRD6989 (5 µM; 24 hours) enhances IL-10 production in activated human and murine macrophages and dendritic cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 642008-81-9
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Appearance Solid
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Peso molecular 264.33
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Fòrmula C16H16N4
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Color Off-white to light yellow
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SMILES
N#CC1=C(C2=CC=CN=C2)C3=C(N=C1N)CCC(C)C3
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Publications (2)
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Journal Impact Factor
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Most Recent
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J Genet Genomics
Single-nucleotide transcription start sites profiling via nascent strand-specific RNA sequencing uncovers IFN-γ-induced promoter dynamics. [Abstract]2026 Mar 20:S1673-8527(26)00102-5. PMID: 41866111 -
Solvente y solubilidad
DMSO : 25 mg/mL (94.58 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (277 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.7832 mL | 18.9158 mL | 37.8315 mL | 94.5788 mL |
| 5 mM | 0.7566 mL | 3.7832 mL | 7.5663 mL | 18.9158 mL | |
| 10 mM | 0.3783 mL | 1.8916 mL | 3.7832 mL | 9.4579 mL | |
| 15 mM | 0.2522 mL | 1.2611 mL | 2.5221 mL | 6.3053 mL | |
| 20 mM | 0.1892 mL | 0.9458 mL | 1.8916 mL | 4.7289 mL | |
| 25 mM | 0.1513 mL | 0.7566 mL | 1.5133 mL | 3.7832 mL | |
| 30 mM | 0.1261 mL | 0.6305 mL | 1.2611 mL | 3.1526 mL | |
| 40 mM | 0.0946 mL | 0.4729 mL | 0.9458 mL | 2.3645 mL | |
| 50 mM | 0.0757 mL | 0.3783 mL | 0.7566 mL | 1.8916 mL | |
| 60 mM | 0.0631 mL | 0.3153 mL | 0.6305 mL | 1.5763 mL | |
| 80 mM | 0.0473 mL | 0.2364 mL | 0.4729 mL | 1.1822 mL |
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