AC-45594
Based on 1 Customer Validation
AC-45594 (4-(Hexyloxy)phenol) is a UPR activator. AC-45594 induces endoplasmic reticulum stress, activates the unfolded protein response (UPR), and drives the transition from adaptive stress signaling to terminal stress signaling, ultimately leading to cell Apoptosis. AC-45594 activates Caspase-3, induces PARP cleavage, and increases the protein level of DR5. AC-45594 selectively inhibits the growth of Ewing sarcoma cells.
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- Purity: 99.94%
- CAS No.: 18979-55-0
- 화학식: C12H18O2
- 분자량:194.27
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보관:
RT, stored under nitrogen.
In solvent -80°C, 1 year , -20°C, 6 months
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
All Caspase Isoforms
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Biological Activity
제품 설명
IC50 & Target
[1]|
Caspase 3 |
Caspase 8 |
Caspase-7 |
In Vitro
AC-45594 (72 h) selectively inhibits the growth of Ewing sarcoma cell lines COG-E-352, SK-ES-1, TC-32, SKNMC, TC-71 and TC-106 in a concentration-dependent manner, with EC50 values ranging from 45.0 ± 2.2 to 144.3 ± 13.0 nM[1].
AC-45594 (25-200 nM; 72 h) induces apoptosis in SK-ES-1 and TC-71 Ewing sarcoma cell lines, while reducing the cell populations in the S and G2/M phases[1].
AC-45594 (200 nM; 8-16 h) triggers sequential activation of the unfolded protein response and pro-apoptotic signaling in SK-ES-1 Ewing sarcoma cells, with early stress response changes observed at 8 h post-treatment, and extensive UPR activation and apoptosis initiation detected at 16 h post-treatment[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Ewing sarcoma cell lines (SK-ES-1, TC-71)
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Concentration:25, 50, 100 nM
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Incubation Time:72 h
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Result:Did not alter cell cycle distribution in SK-ES-1 or TC-71 cells at 50 nM (below EC50 values for both cell lines).
Significantly reduced the proportion of SK-ES-1 cells in S (12.97 ± 0.32% vs 21.83 ± 0.49% in control) and G2/M (20.27 ± 0.03% vs 22.57 ± 0.15% in control) phases, and increased apoptotic cells to 4.70 ± 0.12% at 100 nM.
Reduced the proportion of TC-71 cells in S (12.97 ± 0.32% vs 20.60 ± 0.90% in control) and G2/M (16.20 ± 0.38% vs 25.47 ± 0.27% in control) phases, and increased apoptotic cells to 20.23 ± 0.20% at 100 nM.
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Cell Line:Ewing sarcoma cell lines (SK-ES-1, TC-71)
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Concentration:100, 200, 300 nM (72 h); 200 nM (8, 16, 24, 48 h)
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Incubation Time:8, 16, 24, 48, 72 h
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Result:Activated caspase-3 and induced PARP cleavage in TC-71 and SK-ES-1 cells at 100 nM or higher.
Activated caspase-3, -7, and -8, induced PARP cleavage, and increased DR5 protein levels as early as 8 hours in both cell lines at 200 nM.
Chemical Information
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CAS No. 18979-55-0
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Appearance Solid
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분자량 194.27
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화학식 C12H18O2
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Color Off-white to yellow
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SMILES
CCCCCCOC1=CC=C(O)C=C1
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Synonyms
4-(Hexyloxy)phenol
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선적
Room temperature in continental US; may vary elsewhere.
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보관
RT, stored under nitrogen
In solvent -80°C 1 year -20°C 6 months
용액&용해도
In Vitro:
DMSO : 100 mg/mL (514.75 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (12.87 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (270 KB)
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SDS (476 KB)
- English - EN (476 KB)
- Français - FR (476 KB)
- Deutsch - DE (476 KB)
- Norwegian - NO (476 KB)
- Español - ES (476 KB)
- Swedish - SV (476 KB)
- Italian - IT (476 KB)
- Korean - KR (476 KB)
- Portuguese - PT (476 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 1 year; -20°C, 6 months. When stored at -80°C, please use it within 1 year. When stored at -20°C, please use it within 6 months.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 5.1475 mL | 25.7374 mL | 51.4748 mL | 128.6869 mL |
| 5 mM | 1.0295 mL | 5.1475 mL | 10.2950 mL | 25.7374 mL | |
| 10 mM | 0.5147 mL | 2.5737 mL | 5.1475 mL | 12.8687 mL | |
| 15 mM | 0.3432 mL | 1.7158 mL | 3.4317 mL | 8.5791 mL | |
| 20 mM | 0.2574 mL | 1.2869 mL | 2.5737 mL | 6.4343 mL | |
| 25 mM | 0.2059 mL | 1.0295 mL | 2.0590 mL | 5.1475 mL | |
| 30 mM | 0.1716 mL | 0.8579 mL | 1.7158 mL | 4.2896 mL | |
| 40 mM | 0.1287 mL | 0.6434 mL | 1.2869 mL | 3.2172 mL | |
| 50 mM | 0.1029 mL | 0.5147 mL | 1.0295 mL | 2.5737 mL | |
| 60 mM | 0.0858 mL | 0.4290 mL | 0.8579 mL | 2.1448 mL | |
| 80 mM | 0.0643 mL | 0.3217 mL | 0.6434 mL | 1.6086 mL | |
| 100 mM | 0.0515 mL | 0.2574 mL | 0.5147 mL | 1.2869 mL |
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