Fenbendazole
Based on 8 publication(s) in Google Scholar
Fenbendazole is an orally active benzimidazole anthelmintic agent, with a broad antiparasitic range. Fenbendazole is a microtubule destabilizing agent and acts on helminthes primarily by binding to tubulin and disrupting the tubulin microtubule equilibrium. Fenbendazole stabilizes the transcriptional activator HIF-1α. Fenbendazole possesses an efficient anti-proliferative activity and induces apoptosis. Fenbendazole causes cell-cycle arrest and mitotic cell death, and has antitumor activity in mice xenografted with wild-type p53.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- Purity: 99.94%
- CAS No.: 43210-67-9
- 화학식: C15H13N3O2S
- 분자량:299.35
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보관:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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용액&용해도
- 순도&문서
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fenbendazole
More- ACS Environ Au. 2025 Aug 5;5(6):573-582. [Abstract]
- Anal Chem. 2025 Jun 3;97(21):11099-11109. [Abstract]
- Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
- Commun Biol. 2024 Jan 24;7(1):123. [Abstract]
- J Pathol. 2023 Jun;260(2):203-221. [Abstract]
- Molecules. 2026 Mar 5;31(5):864. [Abstract]
- J Vet Sci. 2020 Sep;21(5):e72. [Abstract]
- Research Square Preprint. 2021 Aug.
Customer Validation & Images
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RT-PCR
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WB
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Cell Imaging/Staining
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Cell Proliferation/Viability Assay
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Bio/Physico-chemical Assay
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Biological Activity
제품 설명
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Hepatocyte | IC50 |
3 x 104 nM
Compound: Fenbendazole
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Antimicrobial activity against Plasmodium yoelii 265 liver infected in mammalian hepatocytes after 48 hrs
Antimicrobial activity against Plasmodium yoelii 265 liver infected in mammalian hepatocytes after 48 hrs
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[PMID: 18212104] |
| HepG2 | EC50 |
10 μM
Compound: Fenbendazole
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Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
Activation of human PXR expressed in human HepG2 (DPX-2) cells after 24 hrs by luciferase reporter gene based luminescent analysis
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[PMID: 20966043] |
| HepG2 | EC50 |
4 μM
Compound: Fenbendazole
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Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis
Activation of rat PXR expressed in human HepG2 cells after 24 hrs by luciferase reporter gene based luminescent analysis
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[PMID: 20966043] |
| PC-3M | EC50 |
2.6 μM
Compound: Fenbendazole
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Cytotoxicity against human PC3M cells assessed as reduction in cell viability after 48 hrs by cyquant reagent based fluorescence spectrometric assay
Cytotoxicity against human PC3M cells assessed as reduction in cell viability after 48 hrs by cyquant reagent based fluorescence spectrometric assay
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[PMID: 29288939] |
In Vitro
Fenbendazole (1 uM; for 24 h) significantly reduces cell growth in tumour cell lines with wild-type p53, H460 and A549 human NSCLC cell lines[1].
Fenbendazole (1 uM; for 24 h) induces apoptosis and causes an increased level of p53 protein in the mitochondrial fraction[1].
Fenbendazole (1 uM; for 24 h) causes cell cycle arrest in the mitotic phase in human NSCLC cells[1].
Fenbendazole (1 uM; for 24 h) causes a partial alteration of the microtubule network in human non small cell lung carcinoma (NSCLC) A549 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:A549 cells
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Concentration:1 uM
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Incubation Time:For 24 h
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Result:Caused an early elevation of cyclin B1/CDK1 levels (8 h as compared to 16 h in case of control untreated cells).
p-histone H3 (Ser10) was found to be up-regulated at 12 and 24 h.
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Cell Line:A549 cells
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Concentration:1 uM
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Incubation Time:8, 16, 24, 32, 40, 48 h
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Result:The number of apoptotic cells increased in a time dependent manner with simultaneous decrease in cyclin B1 levels, and ~30% cells had undergone apoptosis after 32 h.
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Cell Line:H460 cells
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Concentration:1 uM
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Incubation Time:For 24 h
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Result:Caused an increased level of p53 protein in the mitochondrial fraction.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Female athymic nu/nu mice were xenografted with A549 cells[1]
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Dosage:1 mg/mouse
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Administration:Orally; every second day for 12 day
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Result:Led to a marked reduction in tumour size and weight.
Led to a reduction in hemoglobin content in tumors signifying reduced tumor vascularity.
Chemical Information
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CAS No. 43210-67-9
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Appearance Solid
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분자량 299.35
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화학식 C15H13N3O2S
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Color White to off-white
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SMILES
O=C(OC)NC1=NC2=CC=C(SC3=CC=CC=C3)C=C2N1
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (8)
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Journal Impact Factor
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Most Recent
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ACS Environ Au
Machine Learning-Assisted Recognition of Environmental Sulfur-Containing Chemicals in Nontargeted Mass Spectrometry Analysis of Inadequate Mass Resolution. [Abstract]2025 Aug 5;5(6):573-582. PMID: 41277996 -
Anal Chem
Exposome-Scale Investigation of Cl-/Br-Containing Chemicals Using High-Resolution Mass Spectrometry, Multistage Machine Learning, and Cloud Computing. [Abstract]2025 Jun 3;97(21):11099-11109. PMID: 40401576 -
Redox Rep
HIF1A/BNIP3 pathway affects ferroptosis in sepsis-induced cardiomyopathy through binding to BCL-2. [Abstract]2025 Dec;30(1):2544412. PMID: 40779707
Fenbendazole purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
Quantification of HIF1A and BNIP3 mRNA levels using qRT-PCR. Fenbendazole (FZ; 1 μM; 48 h) increased HIF1A and BNIP3 mRNA levels.
Fenbendazole purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
Western blot analysis of HIF1A/BNIP3 pathway protein expression in H9c2 cardiomyocytes. Fenbendazole (FZ; 1 μM; 48 h) further increased HIF1A and BNIP3 protein levels compared with LPS alone.
Fenbendazole purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
Fenbendazole (FZ; 1 μM; 48 h). Detection of intracellular ROS in H9c2 cells using DHE staining.
Fenbendazole purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
Cell viability was assessed by CCK-8 assay in H9c2 cardiomyocytes after FZ (Fenbendazole; 1 μM; 48 h) treatment. CCK-8 assays showed decreased viability in LPS and LPS + FZ groups.
Fenbendazole purchased from MedChemExpress. Usage Cited in: Redox Rep. 2025 Dec;30(1):2544412. [Abstract]
Fe2+, MDA, and GSH levels were measured in lysates of H9c2 cardiomyocytes after FZ treatment (Fenbendazole; 1 μM; 48 h). Fe2+ and MDA levels were elevated by LPS/FZ and reduced by LW6 or BNIP3 silencing; GSH depletion was likewise reversed.
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Commun Biol
Parbendazole as a promising drug for inducing differentiation of acute myeloid leukemia cells with various subtypes. [Abstract]2024 Jan 24;7(1):123. PMID: 38267545 -
J Pathol
Novel prognostication biomarker adipophilin reveals a metabolic shift in uveal melanoma and new therapeutic opportunities. [Abstract]2023 Jun;260(2):203-221. PMID: 36825655 -
Molecules
2026 Mar 5;31(5):864. PMID: 41828851 -
J Vet Sci
Dewormer drug fenbendazole has antiviral effects on BoHV-1 productive infection in cell cultures. [Abstract]2020 Sep;21(5):e72. PMID: 33016019 -
용액&용해도
In Vitro:
DMSO : 10 mg/mL (33.41 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 1 mg/mL (3.34 mM); Clear solution
This protocol yields a clear solution of ≥ 1 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (10.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
순도&문서
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Data Sheet (278 KB)
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SDS (563 KB)
- English - EN (563 KB)
- Français - FR (563 KB)
- Deutsch - DE (563 KB)
- Norwegian - NO (563 KB)
- Español - ES (563 KB)
- Swedish - SV (563 KB)
- Italian - IT (563 KB)
- Korean - KR (563 KB)
- Portuguese - PT (563 KB)
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Handling Instructions (2659 KB)
References
[1]. Nilambra Dogra, et al. Fenbendazole acts as a moderate microtubule destabilizing agent and causes cancer cell death by modulating multiple cellular pathways. Sci Rep. 2018 Aug 9;8(1):11926. [Content Brief]
[2]. Hossein Aleyasin, et al. Antihelminthic benzimidazoles are novel HIF activators that prevent oxidative neuronal death via binding to tubulin. Antioxid Redox Signal. 2015 Jan 10;22(2):121-34. [Content Brief]
[3]. Qiwen Duan, et al. Fenbendazole as a potential anticancer drug. Anticancer Res. 2013 Feb;33(2):355-62. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 3.3406 mL | 16.7029 mL | 33.4057 mL | 83.5143 mL |
| 5 mM | 0.6681 mL | 3.3406 mL | 6.6811 mL | 16.7029 mL | |
| 10 mM | 0.3341 mL | 1.6703 mL | 3.3406 mL | 8.3514 mL | |
| 15 mM | 0.2227 mL | 1.1135 mL | 2.2270 mL | 5.5676 mL | |
| 20 mM | 0.1670 mL | 0.8351 mL | 1.6703 mL | 4.1757 mL | |
| 25 mM | 0.1336 mL | 0.6681 mL | 1.3362 mL | 3.3406 mL | |
| 30 mM | 0.1114 mL | 0.5568 mL | 1.1135 mL | 2.7838 mL |
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