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Cat. No.: HY-N21633

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Phanginin D

Phanginin D is a cassane-type furanoditerpene found in the seeds of Caesalpinia sappan. Phanginin D induces apoptosis in HL-60 cells by activating Caspase-3 and promoting the cleavage of procaspase-3 and PARP. Phanginin D can be used for research on human promyelocytic leukemia.

For research use only. We do not sell to patients.

CAS No.: 1011528-61-2
Formula: C₂₂H₃₀O₅
Molecular Weight:374.47

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Phanginin D Chemical Structure

CAS No.: 1011528-61-2

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Purity & Documentation
References
Calculators
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Classifications
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Biological Activity

Description

IC₅₀ & Target

^[1]

Caspase 3

All Caspase Isoforms :

All PARP Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
HL-60	IC50	11.7 μM	Cytotoxicity against human HL-60 promyelocytic leukemia cells assessed by modified MTT assay after 48 hrs of treatment. Cytotoxicity against human HL-60 promyelocytic leukemia cells assessed by modified MTT assay after 48 hrs of treatment.	25858626
HeLa	IC50	22.7 μM	Cytotoxicity against human HeLa cervical adenocarcinoma cells assessed by modified MTT assay after 48 hrs of treatment. Cytotoxicity against human HeLa cervical adenocarcinoma cells assessed by modified MTT assay after 48 hrs of treatment.	25858626

In Vitro

Anti-cancerApoptosis

Anti-cancer

Phanginin D (compound 3) (1-50 μM; 48 h) exhibits potent cytotoxic activity against HL-60 cells with an IC₅₀ of 11.7 μM and an IC₅₀ of 22.7 μM against HeLa cells, while showing weaker activity against MCF-7 cells^[1].
Phanginin D (compound 4) shows no activity against the human KB cell line in the SRB cytotoxicity assay^[2].

Anti-cancer

Apoptosis

Phanginin D (1-30 μM; 12-48 h) increases Caspase-3 activity by approximately 1-5 fold in a dose- and time-dependent manner in HL-60 cells^[1].
Phanginin D (1-30 μM; 24 h) promotes procaspase-3 cleavage and Caspase-3 activation in HL-60 cells, and at 30 μM promotes the cleavage of PARP from the 116 kDa intact protein into predominantly an 89 kDa fragment^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis^[1]

Cell Line:

HL-60
Concentration:

1, 10, 30 μM
Incubation Time:

12, 24, 48 h
Result:

Increased caspase-3 activity by approximately 1-5 folds.
Displayed dose- and time-dependent caspase-3 activation.

Western Blot Analysis^[1]

Cell Line:

HL-60
Concentration:

0, 1, 10, 30 μM
Incubation Time:

24 h
Result:

Induced proteolytic cleavage of procaspase-3 into its active form.
Cleaved PARP from its intact form (116 kDa) into fragments (major fragment, 89 kDa) by treatment with 30 μM.

Related Protocols :

Chemical Information

CAS No. 1011528-61-2
Molecular Weight 374.47
Formula C₂₂H₃₀O₅
SMILES

COC([C@]12[C@@]3([H])[C@](CO[C@@H]1OC)(CCC2)[C@]4([H])[C@@]([C@H](C5=C(OC=C5)C4)C)([H])CC3)=O
Structure Classification
- Terpenoids
- Diterpenoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Handling Instructions (2659 KB)

References

[1]. Tran MH, et al. Cytotoxic constituents from the seeds of Vietnamese Caesalpinia sappan. Pharmaceutical biology. 2015;53(10):1549-54.

[2]. Yodsaoue O, et al. Phanginin A-K, diterpenoids from the seeds of Caesalpinia sappan Linn. Phytochemistry. 2008 Mar;69(5):1242-9.

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Purity & Documentation
References

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Phanginin D

All Caspase Isoforms

All PARP Isoforms