(Rac)-Z-FA-FMK 

(Rac)-Z-FA-FMK is the racemate of Z-FA-FMK. (Rac)-Z-FA-FMK is an inhibitor of cathepsin B with a K_i of 1.5 μM. (Rac)-Z-FA-FMK inhibits caspase-2, -3, -6, -7, and -9 with IC₅₀s of 6.147, 15.41, 32.45, 9.077, and 110.7 μM. (Rac)-Z-FA-FMK inhibits the main protease of SARS-CoV-2 replication with an IC₅₀ of 11.39 μM. (Rac)-Z-FA-FMK inhibits the increased IL-1β level induced by LPS and NF-κB transactivation in macrophages^[1][2][3][4].

386.42

C₂₁H₂₃FN₂O₄

96922-64-4

O=C(NC(CC1=CC=CC=C1)C(NC(C)C(CF)=O)=O)OCC2=CC=CC=C2

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Smith, R. A., et al., (1988). Inhibition of cathepsin B by peptidyl aldehydes and ketones: slow-binding behavior of a trifluoromethyl ketone. Biochemistry, 27(17), 6568–6573.  [Content Brief]

  [2]. Lopez-Hernandez, F. J., et al., (2003). Z-FA-fmk inhibits effector caspases but not initiator caspases 8 and 10, and demonstrates that novel anticancer retinoid-related molecules induce apoptosis via the intrinsic pathway. Molecular cancer therapeutics, 2(3), 255–263.  [Content Brief]

  [3]. Zhu, W., et al., (2020). Identification of SARS-CoV-2 3CL Protease Inhibitors by a Quantitative High-Throughput Screening. ACS pharmacology & translational science, 3(5), 1008–1016.  [Content Brief]

  [4]. Schotte, P., et al., (2001). The cathepsin B inhibitor z-FA.fmk inhibits cytokine production in macrophages stimulated by lipopolysaccharide. The Journal of biological chemistry, 276(24), 21153–21157.  [Content Brief]