Triolein

Name: Triolein
Brand: MedChemExpress
SKU: HY-N1981
Rating: 4.5 (1 reviews)

Cat. No.: HY-N1981 Purity: 99.00%: Data Sheet
COA

SDS
Handling Instructions
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Triolein is a symmetric triacylglycerol that reduces upregulation of MMP-1 and has strong antioxidant and anti-inflammatory activities.

For research use only. We do not sell to patients.

CAS No. : 122-32-7

Size	Stock	Quantity
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Liquid
100 mg	In-stock	Estimated Time of Arrival: December 31
500 mg	In-stock	Estimated Time of Arrival: December 31
1 g	In-stock	Estimated Time of Arrival: December 31
5 g	Get quote
10 g	Get quote

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Customer Review

Based on 1 publication(s) in Google Scholar

Other Forms of Triolein:

1 Publications Citing Use of MCE Triolein

•Mol Med. 2024 Dec 6;30(1):242. [Abstract]

RT-PCR

In Vivo Efficacy Study

Histological Imaging/Staining

: Triolein purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Dec 6;30(1):242. [Abstract]; The expression of inflammatory cytokines IL-4, IL-10, TNF-α, IL-1β, IL-6, and IL-18 mRNAs in the ischemic penumbra treated with Triolein (40 mg/kg, p.o.).

: Triolein purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Dec 6;30(1):242. [Abstract]; The effect of Triolein on activation of proinflammatory microglia after ischemic stroke. Iba1, red, a surface marker of microglia; CD86, green, the surface marker of M1 microglia; DAPI, blue, Nuclei treated with Triolein (40 mg/kg, p.o.).

: Triolein purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Dec 6;30(1):242. [Abstract]; Western blot to detect the effect of Triolein (40 mg/kg, p.o.) on LC3-II/I and P62 protein expression in the C57BL/6J ipsilateral cortex after ischemic stroke brain injury.

: Triolein purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Dec 6;30(1):242. [Abstract]; Representative TTC staining assay was performed to evaluate the infarct volume treated with Triolein (10, 20, 40, 80 mg/kg, p.o.).

: Triolein purchased from MedChemExpress. Usage Cited in: Mol Med. 2024 Dec 6;30(1):242. [Abstract]; Nissl staining showing morphological neuronal changes in the ischemic penumbra 3 days after reperfusion treated with Triolein (10, 20, 40, 80 mg/kg, p.o.).

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Human Endogenous Metabolite Microbial Metabolite

Biological Activity
Purity & Documentation
References
Customer Review

Description

Triolein is a symmetric triacylglycerol that reduces upregulation of MMP-1 and has strong antioxidant and anti-inflammatory activities^[1]^[2].

IC₅₀ & Target

Human Endogenous Metabolite

In Vitro

Triolein (20 μM, 48 h) decreases the upregulation of MMP-1 in dermal fibroblasts produced by ROS^[1].
Triolein (10 μM, 24 h) can improve the oxidative stress induced by oxidative low-density lipoprotein in endothelial cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR^[1]

Cell Line:	Tel‐E6E7
Concentration:	20 μM
Incubation Time:	48 h
Result:	Reduced the IL-6 expression and ROS generation.

Clinical Trial

Molecular Weight

885.43

Formula

C₅₇H₁₀₄O₆

CAS No.

122-32-7

Appearance

Liquid (Density: 0.91 g/cm³)

Color

Colorless to light yellow

SMILES

O=C(CCCCCCC/C=C\CCCCCCCC)OC(COC(CCCCCCC/C=C\CCCCCCCC)=O)COC(CCCCCCC/C=C\CCCCCCCC)=O

Initial Source

Endogenous metabolite

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

-20°C, protect from light, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (112.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.1294 mL	5.6470 mL	11.2939 mL
5 mM	0.2259 mL	1.1294 mL	2.2588 mL
10 mM	0.1129 mL	0.5647 mL	1.1294 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.08 mg/mL (2.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

*In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

Purity & Documentation

Purity: 99.00%

Select Batch:

Data Sheet (268 KB) SDS (604 KB)

COA (238 KB)

Handling Instructions (2659 KB)

References

[1]. Leirós GJ, et al. Triolein reduces MMP-1 upregulation in dermal fibroblasts generated by ROS production in UVB-irradiated keratinocytes. J Dermatol Sci. 2017 Feb;85(2):124-130. [Content Brief]

[2]. Luo T, et al. Triolein and trilinolein ameliorate oxidized low-density lipoprotein-induced oxidative stress in endothelial cells. Lipids. 2014 May;49(5):495-504. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	1.1294 mL	5.6470 mL	11.2939 mL	28.2349 mL
	5 mM	0.2259 mL	1.1294 mL	2.2588 mL	5.6470 mL
	10 mM	0.1129 mL	0.5647 mL	1.1294 mL	2.8235 mL
	15 mM	0.0753 mL	0.3765 mL	0.7529 mL	1.8823 mL
	20 mM	0.0565 mL	0.2823 mL	0.5647 mL	1.4117 mL
	25 mM	0.0452 mL	0.2259 mL	0.4518 mL	1.1294 mL
	30 mM	0.0376 mL	0.1882 mL	0.3765 mL	0.9412 mL
	40 mM	0.0282 mL	0.1412 mL	0.2823 mL	0.7059 mL
	50 mM	0.0226 mL	0.1129 mL	0.2259 mL	0.5647 mL
	60 mM	0.0188 mL	0.0941 mL	0.1882 mL	0.4706 mL
	80 mM	0.0141 mL	0.0706 mL	0.1412 mL	0.3529 mL
	100 mM	0.0113 mL	0.0565 mL	0.1129 mL	0.2823 mL

Triolein Related Classifications

Inflammation/Immunology

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Triolein122-32-7Environmental PollutantsMMPEndogenous MetaboliteEnvironmental ContaminantsMatrix metalloproteinasesAnti-inflammatoryAntioxidantTel‐E6E7Endothelial cellsInhibitorinhibitorinhibit

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Triolein

Customer Review

Other Forms of Triolein:

Triolein Related Antibodies

1 Publications Citing Use of MCE Triolein

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All MMP Isoform Specific Products:

View All Endogenous Metabolite Isoform Specific Products:

Biological Activity

Purity & Documentation

References

Customer Review

Triolein Related Antibodies

Molarity Calculator

Dilution Calculator

Complete Stock Solution Preparation Table

Triolein Related Classifications

Keywords:

Your Recently Viewed Products:

Customer Review

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