α5β1 integrin agonist-1
Based on 1 Customer Validation
α5β1 integrin agonist-1 is an α5β1 integrin agonist, with an EC50 of 1.5 nM. α5β1 integrin agonist-1 inhabits α4β1 integrin (IC50 = 2.99 μM) in Jurkat/VCAM-1 adhesion assayα5β1 integrin agonist-1 induces concentration-dependent apoptosis and activates the caspase 3/7 pathway in α5β1 integrin-expressing K562 cancer cells. α5β1 integrin agonist-1 can be used for the study of cancer.
For research use only. We do not sell to patients.
- Purity: 99.67%
- CAS No.: 2756557-83-0
- Formula: C24H26FN5O9
- Molecular Weight:547.49
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
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α5β1 1.30 μM (EC50) |
α4β1 2.99 μM (IC50) |
α5β1 integrin agonist-1 (Compound E) inhabits α4β1 integrin (IC50 = 2.99 μM) and activates α5β1 integrin (EC50 = 1.30 μM) in Jurkat/VCAM-1 adhesion assay[1].
α5β1 integrin agonist-1 (10-100 μM, 72 h) induces concentration-dependent apoptosis and activates caspase 3/7 pathway in K562 cells (expressing α5β1 integrin), but shows no pro-apoptotic effect in Jurkat cells or non-cancerous HEK293 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 cells
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Concentration:10, 50, 100 μM
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Incubation Time:72 h
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Result:Induced concentration-dependent apoptosis and activated caspase 3/7 pathway in K562 cells.
Chemical Information
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CAS No. 2756557-83-0
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Appearance Solid
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Molecular Weight 547.49
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Formula C24H26FN5O9
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Color White to off-white
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SMILES
OC(C[C@H]1N(C([C@]1([H])[C@@H](C)OC(CCNC(CN2C(NC(C(F)=C2)=O)=O)=O)=O)=O)C(NC3=C(C=CC=C3)C)=O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (182.65 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (4.57 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (273 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.8265 mL | 9.1326 mL | 18.2652 mL | 45.6629 mL |
| 5 mM | 0.3653 mL | 1.8265 mL | 3.6530 mL | 9.1326 mL | |
| 10 mM | 0.1827 mL | 0.9133 mL | 1.8265 mL | 4.5663 mL | |
| 15 mM | 0.1218 mL | 0.6088 mL | 1.2177 mL | 3.0442 mL | |
| 20 mM | 0.0913 mL | 0.4566 mL | 0.9133 mL | 2.2831 mL | |
| 25 mM | 0.0731 mL | 0.3653 mL | 0.7306 mL | 1.8265 mL | |
| 30 mM | 0.0609 mL | 0.3044 mL | 0.6088 mL | 1.5221 mL | |
| 40 mM | 0.0457 mL | 0.2283 mL | 0.4566 mL | 1.1416 mL | |
| 50 mM | 0.0365 mL | 0.1827 mL | 0.3653 mL | 0.9133 mL | |
| 60 mM | 0.0304 mL | 0.1522 mL | 0.3044 mL | 0.7610 mL | |
| 80 mM | 0.0228 mL | 0.1142 mL | 0.2283 mL | 0.5708 mL | |
| 100 mM | 0.0183 mL | 0.0913 mL | 0.1827 mL | 0.4566 mL |
α5β1 integrin agonist-1 Related Classifications
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