Art. -Nr.: HY-N6940

Prosapogenin A

Name: Prosapogenin A
Brand: MedChemExpress
SKU: HY-N6940
Price: 125.00 USD
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(Synonyms: Progenin III)

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Prosapogenin A, a natural product from Veratrum, induces apoptosis in human cancer cells in vitro via inhibition of the STAT3 signaling pathway and glycolysis.

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Reinheit : 99.88%
CAS. Nr.: 19057-67-1
Formel: C₃₉H₆₂O₁₂
Molecular Weight:722.90

Speicherung:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Prosapogenin A Chemical Structure

CAS. Nr.: 19057-67-1

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Biologische Aktivität
- Beschreibung
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

Prosapogenin A, a natural product from Veratrum, induces apoptosis in human cancer cells in vitro via inhibition of the STAT3 signaling pathway and glycolysis^[1].

Alle Caspase Isoform-spezifische Produkte anzeigen :

Alle STAT Isoform-spezifische Produkte anzeigen :

Cellular Effect

Cell Line	Type	Value	Description	References
BXPC-3	GI₅₀	0.43 μg/mL Compound: 5	Growth inhibition of humanBxPC3 cells after 48 hrs Growth inhibition of humanBxPC3 cells after 48 hrs	[PMID: 15921418]
DU-145	GI₅₀	>10 μg/mL Compound: 5	Growth inhibition of human DU145 cells after 48 hrs Growth inhibition of human DU145 cells after 48 hrs	[PMID: 15921418]
HeLa	IC₅₀	6.23 μg/mL Compound: 7	Cytotoxicity against human HeLa cells after 72 hrs by MTT assay Cytotoxicity against human HeLa cells after 72 hrs by MTT assay	[PMID: 15620239]
HL-60	IC₅₀	5 μM Compound: 25	Cytotoxicity against human HL60 cells after 72 hrs by MTT assay Cytotoxicity against human HL60 cells after 72 hrs by MTT assay	[PMID: 18023191]
KM-20L2	GI₅₀	1.2 μg/mL Compound: 5	Growth inhibition of human KM20L2 cells after 48 hrs Growth inhibition of human KM20L2 cells after 48 hrs	[PMID: 15921418]
MCF7	GI₅₀	0.26 μg/mL Compound: 5	Growth inhibition of human MCF7 cells after 48 hrs Growth inhibition of human MCF7 cells after 48 hrs	[PMID: 15921418]
NCI-H460	GI₅₀	1.8 μg/mL Compound: 5	Growth inhibition of human NCI-H460 cells after 48 hrs Growth inhibition of human NCI-H460 cells after 48 hrs	[PMID: 15921418]
NCI-H460	IC₅₀	5.04 μM Compound: 15	Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay Cytotoxicity against human NCI-H460 cells incubated for 24 hrs by MTT assay	[PMID: 28606759]
P388	ED₅₀	>10 μg/mL Compound: 5	Growth inhibition of mouse P388 cells after 48 hrs Growth inhibition of mouse P388 cells after 48 hrs	[PMID: 15921418]
SF-268	GI₅₀	1.6 μg/mL Compound: 5	Growth inhibition of human SF268 cells after 48 hrs Growth inhibition of human SF268 cells after 48 hrs	[PMID: 15921418]
SK-MEL-1	IC₅₀	7 μM Compound: 25	Cytotoxicity against human SKMEL1 cells after 72 hrs by MTT assay Cytotoxicity against human SKMEL1 cells after 72 hrs by MTT assay	[PMID: 18023191]
U-937	IC₅₀	6 μM Compound: 25	Cytotoxicity against human U937 cells after 72 hrs by MTT assay Cytotoxicity against human U937 cells after 72 hrs by MTT assay	[PMID: 18023191]

In Vitro

Prosapogenin A (0-15 µM, 48 h) arrests the cell cycle of HepG2 at G2/M phase, inhibits the growth of cancer cells HeLa, MCF-7 and HepG2 with IC₅₀s of 9.27, 9.36 and 8.41 µM, respectively^[1].
Prosapogenin A (2–10 µM, 24 h) activates V-ATPase and caspase 3/8 pathway, triggers increased lysosomal membrane permeability (LMP), and induces a GSDME-dependent pyroptosis in anaplastic thyroid cancer cells 8505C, KHM-5M, C643, BHT-101, and HTh-7^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis^[2]

Cell Line:

HepG2
Concentration:

0-15 µM
Incubation Time:

48 h
Result:

Arrested the cell cycle at G2/M phase.

In Vivo

Prosapogenin A (10-20 mg/kg, ip, once daily for 14 days) exhibits antitumor efficacy in 8505C xenograft mouse models^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

8505C xenograft mouse models^[2]
Dosage:

10-20 mg/kg
Administration:

ip, once daily for 14 days
Result:

Inhibited tumor growth.

Related Protocols :

Chemical Information

CAS. Nr. 19057-67-1
Appearance Solid
Molecular Weight 722.90
Formel C₃₉H₆₂O₁₂
Color White to off-white
SMILES

C[C@@]12[C@]3([H])[C@](O[C@]4(CC[C@@H](C)CO4)[C@H]3C)([H])C[C@@]1([H])[C@@]5([H])[C@]([C@@]6(C(C[C@@H](O[C@@]7([H])[C@@H]([C@H]([C@H](O)[C@@H](CO)O7)O)O[C@@]8([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O8)O)O)CC6)=CC5)C)([H])CC2
Synonyms

Progenin III
Structure Classification
- Steroids
Initial Source
- Plants
- Other Families Extract
Versand

Room temperature in continental US; may vary elsewhere.
Speicherung

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 100 mg/mL (138.33 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3833 mL

6.9166 mL

13.8332 mL

5 mM

0.2767 mL

1.3833 mL

2.7666 mL

10 mM

0.1383 mL

0.6917 mL

1.3833 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: 2.5 mg/mL (3.46 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (288 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Wang TX, et al. Prosapogenin A induces apoptosis in human cancer cells in vitro via inhibition of the STAT3 signaling pathway and glycolysis. Oncol Lett. 2013 Nov;6(5):1323-1328. [Content Brief]

[2]. Liu Y, et al., Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification. Cell Death Dis. 2024 Aug 13;15(8):586. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3833 mL	6.9166 mL	13.8332 mL	34.5829 mL
	5 mM	0.2767 mL	1.3833 mL	2.7666 mL	6.9166 mL
	10 mM	0.1383 mL	0.6917 mL	1.3833 mL	3.4583 mL
	15 mM	0.0922 mL	0.4611 mL	0.9222 mL	2.3055 mL
	20 mM	0.0692 mL	0.3458 mL	0.6917 mL	1.7291 mL
	25 mM	0.0553 mL	0.2767 mL	0.5533 mL	1.3833 mL
	30 mM	0.0461 mL	0.2306 mL	0.4611 mL	1.1528 mL
	40 mM	0.0346 mL	0.1729 mL	0.3458 mL	0.8646 mL
	50 mM	0.0277 mL	0.1383 mL	0.2767 mL	0.6917 mL
	60 mM	0.0231 mL	0.1153 mL	0.2306 mL	0.5764 mL
	80 mM	0.0173 mL	0.0865 mL	0.1729 mL	0.4323 mL
	100 mM	0.0138 mL	0.0692 mL	0.1383 mL	0.3458 mL

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Prosapogenin A

Alle Caspase Isoform-spezifische Produkte anzeigen

Alle STAT Isoform-spezifische Produkte anzeigen