Vacquinol-1
Based on 1 Customer Validation
Vacquinol-1 (NSC13316) is a MKK4-specific activator that activates the MAPK pathway. Vacquinol-1 inhibits the growth, migration and colony formation, and induces apoptosis of cancer cells. Vacquinol-1 is applicable to research related to cancers such as hepatocellular carcinoma.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- Reinheit: 99.33%
- CAS. Nr.: 5428-80-8
- Formel: C21H21ClN2O
- Molecular Weight:352.86
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Speicherung:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Chemical Information
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Lösungsmittel & Löslichkeit
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Immunology/Inflammation Compound Library
HY-L007
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MAPK Compound Library
HY-L010
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Anti-Cancer Compound Library
HY-L025
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Oxygen Sensing Compound Library
HY-L045
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Pyroptosis Compound Library
HY-L059
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Anti-Lung Cancer Compound Library
HY-L075
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Anti-Cancer Metabolism Compound Library
HY-L083
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Neurodegenerative Disease-related Compound Library
HY-L086
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Angiogenesis-Related Compound Library
HY-L088
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Mitochondria-Targeted Compound Library
HY-L089
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Anti-Liver Cancer Compound Library
HY-L101
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Osteogenesis Compound Library
HY-L131
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Heterocyclic Compound Library
HY-L138
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Pain-Related Compound Library
HY-L139
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Multi-Target Compound Library
HY-L176
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Mitophagy Compound Library
HY-L180
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Protein Kinase Compound Library
HY-L196
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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Cell Proliferation Compound Library
HY-L201
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High-Throughput Bioactive Compound Library
HY-L205
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Lactylation Compound Library
HY-L249
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Biologische Aktivität
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| U3013MG | IC50 |
3.14 μM
Compound: 1
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Cytotoxicity against patient-derived U3013MG cells assessed as reduction in cell viability by Celltiter-Glo reagent based assay
Cytotoxicity against patient-derived U3013MG cells assessed as reduction in cell viability by Celltiter-Glo reagent based assay
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[PMID: 27607569] |
Vacquinol-1 (1.56-25 μM; 24-72 h) inhibits the viability of BEL7402/Huh7 hepatocellular carcinoma cells in vitro[1].
Vacquinol-1 (0-24 μM; 24 h) inhibits the colony-forming ability of BEL7402/Huh7 hepatocellular carcinoma cells and induces cell apoptosis[1].
Vacquinol-1 (10 μM; 24 h) activates the endogenous apoptotic pathway in BEL7402 hepatocellular carcinoma cells, induces the cleavage of caspase-3, caspase-9 and PARP-1, upregulates the expression of Bax and Bim, and downregulates the expression of Bcl-2[1].
Vacquinol-1 (10 μM; 1-12 h) induces the translocation of the pro-apoptotic protein Bax from the cytoplasm to mitochondria in BEL7402 hepatocellular carcinoma cells[1].
Vacquinol-1 (15 μM; 5 h) activates the JNK and p38 MAPK pathways, upregulates α-SMA, an activation marker of the hepatic stellate cell line HSC-T6, promotes collagen production, and induces phosphorylation of the linker region of Smad2/3[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited BEL7402 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 12.35 μM (24 h), 11.48 μM (48 h), and 10.21 μM (72 h).
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Cell Line:Huh7 (hepatocellular carcinoma, HCC)
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Concentration:1.56 μM, 3.13 μM, 6.26 μM, 12.5 μM, 25 μM
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Incubation Time:24, 48, 72 h
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Result:Inhibited Huh7 cell proliferation in a dose-dependent manner.
Reached half maximal inhibitory concentration (IC50) values of 6.21 μM (24 h), 5.58 μM (48 h), and 4.89 μM (72 h).
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Cell Line:BEL7402 (hepatocellular carcinoma, HCC)
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Concentration:10 μM
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Incubation Time:24 h
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Result:Increased the percentage of apoptotic cells.
Resulted in nuclear fragmentation and apoptotic body formation in BEL7402 cells, whereas control cells displayed round, normal nuclei.
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Cell Line:rat hepatic stellate HSC-T6 cells
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Concentration:15 μM
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Incubation Time:5 h
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Result:Upregulated the protein levels of phosphorylated (P)-JNK1/2 and P-p38 (but not P-ERK1/2) in HSC-T6 cells.
Increased the protein levels of α-SMA, Collagen I, P-Smad2L, and P-Smad3L in HSC-T6 cells.
Chemical Information
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CAS. Nr. 5428-80-8
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Appearance Solid
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Molecular Weight 352.86
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Formel C21H21ClN2O
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Color White to off-white
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SMILES
OC(C1NCCCC1)C2=CC(C3=CC=C(Cl)C=C3)=NC4=CC=CC=C24
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Synonyms
NSC13316
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Lösungsmittel & Löslichkeit
DMSO : 12.5 mg/mL (35.42 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Reinheit & Dokumentation
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Data Sheet (280 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
Verweise
[1]. Sun SL, et al. Vacquinol‑1 induces apoptosis in hepatocellular carcinoma cell. Mol Med Rep. 2018;18(1):557-563. [Content Brief]
[2]. Wu C, et al. Salvianolic acid B exerts anti-liver fibrosis effects via inhibition of MAPK-mediated phospho-Smad2/3 at linker regions in vivo and in vitro. Life Sci. 2019;239:116881. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.8340 mL | 14.1699 mL | 28.3399 mL | 70.8496 mL |
| 5 mM | 0.5668 mL | 2.8340 mL | 5.6680 mL | 14.1699 mL | |
| 10 mM | 0.2834 mL | 1.4170 mL | 2.8340 mL | 7.0850 mL | |
| 15 mM | 0.1889 mL | 0.9447 mL | 1.8893 mL | 4.7233 mL | |
| 20 mM | 0.1417 mL | 0.7085 mL | 1.4170 mL | 3.5425 mL | |
| 25 mM | 0.1134 mL | 0.5668 mL | 1.1336 mL | 2.8340 mL | |
| 30 mM | 0.0945 mL | 0.4723 mL | 0.9447 mL | 2.3617 mL |
Vacquinol-1 Related Classifications
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