Bafilomycin A1

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Feb 17;6(1):67. [Abstract]

Representative images of cells with GFP or GFP-LC3 puncta, and the average number of GFP-LC3 puncta per cell in stable RFP-GFP-LC3 U87 cells treated with Justicidin A (JA) (0.13 μM), Justicidin B (JB) (0.13 μM), or Justicidin C (JC) (4 μM) in the presence or absence of Compound C (2.5 μM), SCH772984 (10 μM), LY294002 (25 μM), or Bafilomycin A1 (1 nM) for 24 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nature. 2022 Aug;608(7922):413-420. [Abstract]

Huh7 cells and ASGR1 KO cells are treated with 20 nM Bafilomycin A1 for 2 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell. 2025 Jun 26;188(13):3441-3458.e25. [Abstract]

Effects of Baf-A1 (Bafilomycin A1, Baf; 100 nM; 30 min) on lysosomal acidity (LysoTracker red assay) in WT and TMEM175 KO HeLa cells (A.U., arbitrary unit). Inhibition of V-ATPase with bafilomycin-A1 resulted in a dramatic reduction of lysosomal acidity (estimated with LysoTracker labeling) in both wild-type (WT) and TMEM175 knockout (KO) cell lines (HeLa and HAP1).

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844. [Abstract]

Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30 nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Mol Cancer. 2024 Sep 2;23(1):184. [Abstract]

Furthermore, transfect NSCLC cells (H1975 and H358) with GFP-PD-L1 plasmid and conduct western blot analysis to investigate the upregulated LAMTOR1’s impact on exosomal PD-L1 expression through the lysosomal degradation pathway. Subsequently, NSCLC cells (H1975 and H358) were treated with OE-LAMTOR1 in the presence or absence of 50 μM Bafilomycin A1 (Baf-A1; 50 μM; 0-90 min) treatment. Baf-A1 significantly increased exosomal PD-L1 expression levels.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2025 Feb;20(2):296-302. [Abstract]

Bafilomycin A1 (100 nM; 24 h) significantly reduces TPD-NP-based EGFR degradation in MDA-MB-231 cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2022 Sep;17(9):993-1003. [Abstract]

Immunofluorescence (IF) images of phagocytosis and elimination of SC2-P (red) in the absence or presence of CIPS in THP-1 differentiated macrophages. The lysosomal inhibitor Bafilomycin A1 (BM; 100 nM; 6 h) is added to macrophages 6 h before the end of culture.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2025 Oct 7:S1550-4131(25)00389-4. [Abstract]

LC3B-II and p62 were compared in neurons isolated from WT, Gcn2^−/−, α2^−/−, and α1^−/− mice with or without Bafilomycin (Bafilomycin A1; 50 nM; 4 h) treatment. The quantification of LC3II/LC3I and p62/Actin was shown below.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2021 May 4;33(5):971-987.e6. [Abstract]

Western blot detection of YAP expression in HEK293T cells transfected with siControl or siATP6V0d2, followed by treatment with 100 nM bafilomycin A1 (BafA1) for 6 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Nov 11;58(11):2670-2684.e10. [Abstract]

L929 cells and RAW cells were treated with Baf-A1 (Bafilomycin A1; 1 μM) for 12 h, and the expression levels of SMPDL3B were determined by immunoblotting. Baf-A1 markedly increased SMPDL3B protein abundance.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Immunity. 2025 Apr 8:S1074-7613(25)00128-1. [Abstract]

Th17-skewing T cells pre-treated with Baf-A1 (Bafilomycin A1; 10 nM; pretreatment for 30 min followed by incubation with PKH-26-labeled A. faecalis-OMVs overnight) were analyzed for OMVs in EEA1⁺ early endosomes by confocal microscopy. Baf-A1 remarkably prevented the internalization of A. faecalis OMVs by CD4⁺ T cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2022 Feb 14;9(1):9. [Abstract]

GL261 and U251 cells are treated with 3-MA (5 mM), BafA1 (10 nM), AG-205 (10 µM), or RAPA (20 nM) for 1 h followed by IR or IR plus UTMD treatment for another 24 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Aug;45(8):1010-1037. [Abstract]

Western blotting analysis of PD-L1 expression of PCSK9 knockout KPC cells treated with 50 nmol/L Baf-A1 (Bafilomycin A1; 0-16 h). When Baf-A1 was used to inhibit lysosome function, PD-L1 levels significantly accumulated in both control and PCSK9-knockout cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cancer Commun (Lond). 2025 Mar;45(3):218-244. [Abstract]

Western blots of the products of transfected ubiquitination using cell lysate from LNCaP cells. Cells were transfected with the indicated constructs and treated with 1 μmol/L Baf-A1 (Bafilomycin A1) for 16 h. Baf-A1 increased CD86 ubiquitination in RT-resistant PCa cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Immunol. 2025 Apr 11. [Abstract]

Representative histogram of FDG fluorescence and quantification of FDG mean fluorescence intensity (MFI) in human and mouse intratumor neutrophils in male and female. Bafilomycin A1 (100 nM; 1 h pretreatment followed by 2 h incubation after FDG addition) was used to induce lysosomal alkalinization.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Gut. 2025 Apr 23:gutjnl-2025-335220. [Abstract]

Immunoblots for autophagy/mitophagy-related proteins in Kras;Gnas 4301 and 4861 cells after treatment with Bafilomycin A1 (BAF; 100 nM; 24 h), alone or with UGT8-IN-1 (10 μM). Bafilomycin A1 + UGT8-IN-1 increased the accumulation of p62 and LC3B compared with Bafilomycin A1 alone.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2025 May;22(5):512-526. [Abstract]

Western blot analysis of p62 and LC3 protein levels in sgCtrl or Gpnmb-knockout THP-1 cells infected with M. marinum (MOI of 10) for 0–6 h, followed by Bafilomycin A1 (BafA1; 1 μM; 6 h) treatment for 6 h. Bafilomycin A1 treatment restored LC3-II levels in Gpnmb-knockout THP-1 cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Jan;19(1):67-78. [Abstract]

HeLa cells transfected with the ORF10-GFP plasmid and pEGFP-N1 are stimulated by poly(I:C) transfection and are then treated with MG132 (5 μM), Bafilomycin A1 (Baf A1; 5 nM), or Chloroquine (CQ; 40 μM) for 24 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jun 3;28(6):1074-1089.e7. [Abstract]

Viability determined by cell counts of P, SA, and SAR iPSC-HSPCs treated with the V-ATPase inhibitor Bafilomycin A1 (Baf A1; 10 nM; 48 hours) compared with untreated cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Host Microbe. 2026 Apr 8;34(4):657-671.e7. [Abstract]

Western blotting of LC3B in KPC-luc cells after treatment with B. longum and rapamycin, along with or without Bafilomycin A1 (100 nM; 4 h). The combination of B. longum and rapamycin further elevated the LC3B II:LC3B I ratio in the presence of Bafilomycin A1.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2026 Feb;15(2):e70238. [Abstract]

Quantification of S. aureus level in A549 cells with or without Bafilomycin A1 (10 nM; 30 min) treatment across SA and AEVs-SA groups. Bafilomycin A1 effectively suppressed intracellular bacterial colonization within A549 cells treated with AEVs-S. aureus complexes.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2021 Oct;10(12):e12153. [Abstract]

Confocal microscopy analysis of the MVB marker CD63 in Coro1a‐Flag or Coro1a‐K233R‐Flag overexpressing HeLa cells treated with DMSO or 20 nM Bafilomycin A1 (Baf A1) for 12 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Aug 28;12(1):49. [Abstract]

Immunoblots of the CTSK pre-proenzyme and active forms in osteoclasts treated with 10 nmol/L oligomycin A, with or without the lysosome inhibitor Bafilomycin A1 (10 ng/mL; 4 h).

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2026 Jul;22(7):1639-1656. [Abstract]

HeLa cells transfected with the empty vector or EXOC5-HA, followed by HSV-1 infection for 12 h. Protein lysates of the cells treated with BafA1 (Bafilomycin A1; 5 nM) for 12 h. The cell lysates were then analyzed by western blotting. BafA1 blocked EXOC5-mediated STING1 degradation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2026 Jun 14:1-16. [Abstract]

Protein expression was analyzed by western blotting in HEK293T cells transfected with Flag-tagged ORF44 and HA-tagged PFN1 or empty vector, followed by Baf A1 (Bafilomycin A1; 0.4 μM; 6 h) treatment. The PFN1-mediated degradation of ORF44 was inhibited by the autophagy inhibitor Bafilomycin A1.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2026 Jun 3:1-31. [Abstract]

Quantification of WNT2B relative protein levels in HeLa cells treated with Bafilomycin A1 (100 nM; 3 h) and subjected to starvation for the indicated times (n = 5). Blocking autophagosome-lysosome fusion with bafilomycin A1 further decreased intracellular WNT2B.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2026 May 7:1-17. [Abstract]

Protein levels were detected in 293T cells expressing OPTN[LIR]E after treatment with Baf-A1 (Bafilomycin A1; 10 μM; 24 h). Baf-A1 significantly inhibited the degradation of OPTN[LIR]E.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2026 May 15:1-16. [Abstract]

LC3 and SQSTM1/p62 were analyzed by immunoblotting in HeLa and 3D4/21 cells transfected with empty vector or an HA-I10L-expressing plasmid for 20 h, followed by Baf-A1 (Bafilomycin A1; 250 nM; 4 h) treatment. When autophagy was inhibited by CQ or Baf-A1, the levels of LC3-II and SQSTM1/p62 increased

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):3142-3164. [Abstract]

Bafilomycin A1 (BafA1; 0.1 μM; 3 h). Immunoblots and quantification of LC3 protein in HsMSCs and mouse primary hepatocytes.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):2614-2632. [Abstract]

HepG2 cells were treated with 50 nM Bafilomycin A1 or/and 150 μM PA for 6 and 12 h. LC3 and SQSTM1 were analyzed by western blot. Compared with PA treatment alone, LC3-II and SQSTM1 further accumulated in the presence of Bafilomycin A1.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec 8. [Abstract]

Immunoblot analysis of ATP6V1D, ATP6V0D1, and LAMP2 in membrane fractions of RB1CC1^−/−-HeLa cells treated with 50 μM of AMDE-1, HCP, SFG and NTZ with or without Baf (Bafilomycin A1; 0.5 μM) for 2 h.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):3123-3141. [Abstract]

Quantification of autophagic flux. Representative images from the cells cultured in normal, EBSS medium (2 h), or EBSS medium (2 h) supplemented with Bafilomycin A1 (100 nM). Further treatment with Bafilomycin A1 led to a significant accumulation of autophagosomes.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Dec;21(12):3344-3360. [Abstract]

Protein levels of autophagy-related genes and STING1 signaling pathway in mouse chondrocytes infected with Lv-shNC or Lv-shAtg7 treated with or without 200 nM BafA1 (Bafilomycin A1) for 4 h. BafA1 further increased STING1 and p-TBK1 levels in Lv-shNC cells but not in Lv-shAtg7 groups.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Oct;21(10):2130-2147. [Abstract]

Representative images and fluorescence intensities detected via FACS of acidic lysosomes in HT29 cells following F2RL1 knockdown, with BafA1 (Bafilomycin A1; 100 nM; 12 h) employed as a control to disrupt lysosomal acidification.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Aug;21(8):1644-1661. [Abstract]

HEK293T cells in 12-well plates were transfected with the E protein, followed by treatment with DMSO or BafA1 (Bafilomycin A1; 100 nM; 12 h) at 12 hpt. At 24 hpt the cells were then collected, and protein expression was evaluated via WB.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Aug 3:1-20. [Abstract]

Western blotting analysis in UMRC-6 cells reconstituted with an empty vector (EV), wild-type BAP1, or the p.C91S mutant after Bafilomycin A1 (BafA1; 100 nM; 3 h) treatment. When cells are treated with Bafilomycin A1, a similar accumulation of p62 is observed in all cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Aug;21(8):1824-1842. [Abstract]

NSD2-knockdown MDA-MB-231 and BT549 cells or control cells were treated with 5 nM BAF (Bafilomycin A1) for 24 h, the expression of NSD2 and LC3B were measured by western blotting. BAF significantly increased LC3B accumulation in NSD2-knockdown MDA-MB-231 and BT549 cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Apr;21(4):807-826. [Abstract]

Western blotting and quantitative analysis of total HsMAPT, MAP1LC3-II, and SQSTM1 protein levels in HEK293WT cells co-transfected with ISG15 siRNA (si-ISG15) and p3×flag-mapt-3flag (HsMAPT) for 24 h and then treated with Baf A1 (Bafilomycin A1; 100 μM; 24 h). Baf A1 reversed the decreased levels of MAPT, MAP1LC3-II, and SQSTM1 induced by ISG15 downregulation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2025 Apr;21(4):771-788. [Abstract]

Western blot analysis of SQSTM1, LC3, total RAB7A, and GTP-RAB7A (immunoprecipitated by anti-GTP-RAB7A antibody) in four groups as indicated in THP-1 cells. Cells were treated with CID-1067700 (100 μM), ML-098 (100 nM), or Baf A1 (50 nM) for 8 hours.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec;20(12):2697-2718. [Abstract]

Cellular PRRSV-N protein levels were detected by western blot in PRRSV-infected Marc-145 cells incubated with BafA1 (Bafilomycin A1; 50 nM; 24 h). BafA1 downregulated PRRSV-N levels to a great extent.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2021 Dec;17(12):4401-4422. [Abstract]

Abundance of LC3 puncta as reflected by GFP and RFP fluorescence in transiently transfected tandem GFP-RFP-LC3 HeLa cells under different conditions indicated. Baf-A1 (Bafilomycin A1; 1 μM; 4 h) was used as a negative control for inhibiting autophagy. Baf-A1 treatment increased yellow puncta, suggesting blockage in the late step of autophagy.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Feb 6;17(1):2434. [Abstract]

Representative images of and quantification LysoTracker staining in primary peritoneal macrophages pretreated with or without Baf-A1 (Bafilomycin A1; 1 μM) for 1 h, followed by infection with H37Rv or H37RvΔChp2 (MOI = 5) for 4 h. Baf-A1 significantly reduced lysosomal acidification in H37Rv-infected macrophages and abolished the difference in acidification levels between H37Rv and H37RvΔChp2.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2026 Feb 9;17(1):2544. [Abstract]

Western blot of OE-CD38 5-8 F/CNE2 cells after 6 h treatment with BafA1 (Bafilomycin A1; 0.2 μM). Bafilomycin A1 blocked CD38-mediated MAVS degradation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Nov 19;16(1):10181. [Abstract]

WB analyses of LC3 and p62 levels in NC and KD cells after treatment with Bafilomycin A1 (Baf; 1 μm; 18 h), an inhibitor for autophagosome-lysosome fusion. Relative levels of LC3II/LC3I and p62 were presented. The levels of LC3II and p62 were also increased after treatment with Bafilomycin A1 to block autophagosome–lysosome fusion.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Jul 24;16(1):6777. [Abstract]

Representative western blot analysis of wild-type (WT) and PEAK1 knockout (KO) Caco-2 cells treated with 100 nM Bafilomycin A1 (BafA1, autophagy inhibitor) for 12 h. Bafilomycin A1 rescued ZO-1 levels in PEAK1-knockout Caco-2 cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2025 Feb 24;16(1):1917. [Abstract]

A549 cells were treated with Baf A1 (Bafilomycin A1; 100 nM) or DMSO for 6 h, and then stained with Nile red (1 μM) for 30 min, after which the cells were collected and analyzed by flow cytometry. When cells were treated with Bafilomycin (Baf-A1), a vacuolar ATPase inhibitor which inhibits lysosome fusion with autophagosome or endosome, there was a significant augmentation in the fluorescence intensity of Nile red-labeled cells.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Commun. 2024 Sep 4;15(1):7711. [Abstract]

qPCR quantification of the intracellular baculovirus genome GP64 gene after Bac-EGFP transduction in wild-type HeLa cells in the presence or absence of BafA1 (Bafilomycin A1). In BafA1 treatment group, cells are pre-treated with 100 nM BafA1 for 1 h before transduction. Pre-treated cells are transduced with Bac-EGFP at an MOI of 2 for 1 h in the presence of 100 nM BafA1. The virus-transduced cells are maintained in the presence of 10 nM BafA1 for 0-72 h. Pretreatment with BafA1 prolonged the retention of intracellular Bac genome.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: ACS Nano. 2026 May 13. [Abstract]

Lysosomal inhibition assay of MIRV transfection. Representative fluorescence images of SKOV3 cells transfected with MIRV (pEGFP) in the absence or presence of Bafilomycin A1. SKOV3 cells were preincubated with the lysosome inhibitor Bafilomycin A1 (0.1-0.2 μM) for 2 h. Following this pretreatment, cells were exposed to MIRV for 48 h. Bafilomycin A1 significantly attenuated EGFP transfection efficiency in a dose-dependent manner.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2026 Apr 17:S2090-1232(26)00346-2. [Abstract]

Western blot analysis showing p62 protein expression levels in HD-MSCs, HD-MSCs + BafA1 (Bafilomycin A1; 200 nmol/L; 24 h), SOP-MSCs and SOP-MSCs + BafA1. In HD-MSCs, BafA1 resulted in robust p62 accumulation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: J Adv Res. 2026 Feb 21:S2090-1232(26)00175-X. [Abstract]

Immunofluorescence staining of p62 (green) and LC3B (red) in porcine splenic lymphocytes. Baf-A1 (Bafilomycin A1; 20 nM; 12 h) treatment increased the fluorescence intensity of p62 and LC3B.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2026 Feb 24;12(1):13. [Abstract]

NCI-H1299 and NCI-H1975 cells with stable empty vector or ZDHHC14 expression were incubated with 0.1 μM Bafilomycin A1 (BafA1) for 6 h. The collected cell lysates were measured using ASCT2 and Actin antibodies. BafA1 blocked ZDHHC14-mediated ASCT2 degradation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2022 May 3;8(1):40. [Abstract]

The levels of mitochondrial proteins including TIM23, TOM20, and TOM22 decreased along with decreased O-GlcNAcylation levels, which can be inhibited by BafA1.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Oct:86:103793. [Abstract]

Western blot analysis of LC3B and p62 for whole cell lysates from HBE cells after ARSK knockdown with or without BafA1 (Bafilomycin A1; 100 nM; 12 h) treatment. BafA1 experiment demonstrated that reduced LC3BII expression following ARSK knockdown resulted from decreased autophagic flux rather than compromised lysosomal degradation.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Sep:85:103751. [Abstract]

Immunoblots of LC3B I/II, Tom20, and VDAC1 in shNC or shD3 myoblasts treated with LPS ± Bafilomycin A1 (40 nM; 8 h). Under Bafilomycin A1 treatment, LC3B-II accumulated in both groups, while Tom20 and VDAC1 accumulation was more pronounced in the shD3+LPS group.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Redox Biol. 2025 Feb:79:103456. [Abstract]

MOVAs were treated with PDGF (20 ng/mL) in the presence or absence of BafA1 (Bafilomycin A1; 50 nM) for 24 h. The protein levels of COL1, cyclinD1, and PCNA were determined by Western blot. BafA1 suppressed the PDGF-induced upregulation of COL1, PCNA, and cyclin D1.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Mol Cell. 2026 Feb 25:S1097-2765(26)00097-3. [Abstract]

Proliferation of glutamine-starved K562 treated with inhibitors of the autophagy (Bafilomycin A1, Bafilomycin, BAF; 5 μM; 4–5 d). Bafilomycin decreased proliferation upon glutamine depletion despite pyruvate addition.

Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Chem Biol. 2025 Apr;21(4):490-500. [Abstract]

Immunofluorescence images showing delayed SG disassembly under BafA1 (Bafilomycin A1; 100 nM; 3 h pretreatment + 30 min SG formation + 120 min recovery) treatment at 120 min of recovery. BafA1 delayed SG disassembly compared with control.