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Bafilomycin A1

(別名: バフィロマイシン a1; BafA1; バフロマイシン)
815 Cited Publications
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Based on 815 publication(s) in Google Scholar

Bafilomycin A1 (BafA1) is a specific and reversible inhibitor of vacuolar H+-ATPase (V-ATPase) with IC50 values of 4-400 nmol/mg. Bafilomycin A1, a macrolide antibiotic, is also used as an autophagy inhibitor at the late stage. Bafilomycin A1 blocks autophagosome-lysosome fusion and inhibits acidification and protein degradation in lysosomes of cultured cells. Bafilomycin A1 induces apoptosis.

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  • 純度: 99.95%
  • CAS 番号: 88899-55-2
  • 分子式: C35H58O9
  • 分子量:622.83
  • 保管条件:

    -20°C, protect from light

    * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

  • Bafilomycin A1 Chemical Structure
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Bafilomycin A1 Chemical Structure
    Bafilomycin A1 構造式
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
  • Experimental Validation Results for Bafilomycin A1
CAS 番号: 88899-55-2
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  • Bafilomycin A1 Chemical Structure
    Bafilomycin A1 Chemical Structure
    1/16
  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Autophagy. 2024 Dec 11:1-18. [Abstract]

    Customer Validation
     Western blot analysis of SQSTM1, LC3, total RAB7A, and GTP-RAB7A (immunoprecipitated by anti-GTP-RAB7A antibody) in four groups as indicated in THP-1 cells. Cells were treated with CID-1067700 (100 μM), ML098 (100 nM), or Baf A1 (50 nM) for 8 hours.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2025 Feb;20(2):296-302. [Abstract]

    Customer Validation
     Bafilomycin A1 (100 nM; 24 h) significantly reduces TPD-NP-based EGFR degradation in MDA-MB-231 cells.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Microb Pathog. 2024 Dec:197:107046. [Abstract]

    Customer Validation
     BMDMs pretreated with degarelix (1 μM), aminoguanidine hydrochloride (AGHS, 100 μM), BafA1 (100 nM), NAC (5 mM), Z-VAD (20 μM), or 3-MA (5 mM) were infected with H37Rv (MOI = 2). At different time points after infection, bacterial survival was calculated by dividing the CFU at 24 h by the CFU at 3 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nature. 2022 Aug;608(7922):413-420. [Abstract]

    Customer Validation
     Huh7 cells and ASGR1 KO cells are treated with 20 nM Bafilomycin A1 for 2 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844. [Abstract]

    Customer Validation
     Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30 nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Biotechnol. 2022 Dec;40(12):1834-1844. [Abstract]

    Customer Validation
     Treatment with 249C or the autophagy inhibitor Bafilomycin A1 (BafA1; 30 nM), but not with the autophagy inducer rapamycin, resulted in upregulation of autophagy markers SQSTM1/p62 and LC3-I/II over time.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Nat Nanotechnol. 2022 Sep;17(9):993-1003. [Abstract]

    Customer Validation
     Immunofluorescence (IF) images of phagocytosis and elimination of SC2-P (red) in the absence or presence of CIPS in THP-1 differentiated macrophages. The lysosomal inhibitor Bafilomycin A1 (BM; 100 nM; 6 h) is added to macrophages 6 h before the end of culture.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Mil Med Res. 2022 Feb 14;9(1):9. [Abstract]

    Customer Validation
     GL261 and U251 cells are treated with 3-MA (5 mM), BafA1 (10 nM), AG-205 (10 µM), or RAPA (20 nM) for 1 h followed by IR or IR plus UTMD treatment for another 24 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Sep;288:121743. [Abstract]

    Customer Validation
     Bafilomycin A1 (Baf; 50 nM) treatment significantly increases the percentage of both RFP+-GFP+-LC3 puncta and LC3 puncta in PDLSCs at 12 and 24 h during osteogenic induction, while there is no significant difference in the percentage of RFP+-GFP+-LC3 puncta or LC3 puncta between the Baf-treated I-PDLSCs and cells without the Baf treatment.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Biomaterials. 2022 Sep;288:121743. [Abstract]

    Customer Validation
     In response to the Bafilomycin A1 (Baf; 50 nM) treatment, increases LC3 II expression is observed in PDLSCs, and significant differences between the Baf-treated PDLSCs and cells without the Baf treatment are observed at 6 h and 12 h during osteogenic induction.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Discov. 2022 May 3;8(1):40. [Abstract]

    Customer Validation
     The levels of mitochondrial proteins including TIM23, TOM20, and TOM22 decreased along with decreased O-GlcNAcylation levels, which can be inhibited by BafA1.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Feb 17;6(1):67. [Abstract]

    Customer Validation
     Representative images of cells with GFP or GFP-LC3 puncta, and the average number of GFP-LC3 puncta per cell in stable RFP-GFP-LC3 U87 cells treated with Justicidin A (JA) (0.13 μM), Justicidin B (JB) (0.13 μM), or Justicidin C (JC) (4 μM) in the presence or absence of Compound C (2.5 μM), SCH772984 (10 μM), LY294002 (25 μM), or Bafilomycin A1 (1 nM) for 24 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Signal Transduct Target Ther. 2021 Feb 17;6(1):67. [Abstract]

    Customer Validation
     SHSY5Y cells were treated with JA, JB or JC in the presence or absence of Baf (Bafilomycin A1) (1 nM) at the indicated concentrations for 24 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Metab. 2021 May 4;33(5):971-987.e6. [Abstract]

    Customer Validation
     Western blot detection of YAP expression in HEK293T cells transfected with siControl or siATP6V0d2, followed by treatment with 100 nM bafilomycin A1 (BafA1) for 6 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Stem Cell. 2021 Jun 3;28(6):1074-1089.e7. [Abstract]

    Customer Validation
     Viability determined by cell counts of P, SA, and SAR iPSC-HSPCs treated with the V-ATPase inhibitor Bafilomycin A1 (Baf A1; 10 nM; 48 hours) compared with untreated cells.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Mol Immunol. 2022 Jan;19(1):67-78. [Abstract]

    Customer Validation
     HeLa cells transfected with the ORF10-GFP plasmid and pEGFP-N1 are stimulated by poly(I:C) transfection and are then treated with MG132 (5 μM), Bafilomycin A1 (Baf A1; 5 nM), or Chloroquine (CQ; 40 μM) for 24 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: J Extracell Vesicles. 2021 Oct;10(12):e12153. [Abstract]

    Customer Validation
     Confocal microscopy analysis of the MVB marker CD63 in Coro1a‐Flag or Coro1a‐K233R‐Flag overexpressing HeLa cells treated with DMSO or 20 nM Bafilomycin A1 (Baf A1) for 12 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 3;9(10):1015. [Abstract]

    Customer Validation
     Representative western blot images of LC3 (LC3I and LC3II) in primary PTC are isolated from WT and TRPC6-/- mice after treatment with H2O2 (0.5 mM 12 h) in the presence and absence of Bafilomycin A1 (BAF) (20 nM).

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Oct 9;9(10):1032. [Abstract]

    Customer Validation
     Western blot showing SQSTM1 and LC3 levels in LN229 cells after treatment with 200 μM NSC 697855 (NTZ) and 200 nM Bafilomycin A1 (BAF) for 48 h.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195. [Abstract]

    Customer Validation
     Western analysis of the effect of GIT1 knockdown in lowering the LC3-II level under non-starvation or starvation conditions (1 h) with or without Bafilomycin A1 (Baf, 10 nM) in osteoclasts.

  • 1/16

    Bafilomycin A1 purchased from MedChemExpress. Usage Cited in: Cell Death Dis. 2018 Dec 13;9(12):1195. [Abstract]

    Customer Validation
     Western analysis of the effect of GIT1 overexpression in increasing the LC3-II level under basal or starvation conditions with or without Bafilomycin A1 (Baf, 10 nM) in HEK293T cells.

  • Bafilomycin A1