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Cat. No.: HY-P99275

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Biologically Validated

Patritumab

(Synonyms: AMG-888; U3-1287)

2 Cited Publications

고객리뷰

Based on 2 publication(s) in Google Scholar

Patritumab (Human Anti-ERBB3 Recombinant Antibody) is a neutralizing monoclonal antibody to ERBB3. Patritumab shows a synergy with Cetuximab (HY-P9905), potently inhibits the phosphorylation of EGFR, HER2, HER3, ERK, and AKT. Patritumab also induces cell apoptosis and suppresses the growth of pancreatic, non-small cell lung cancer, and colorectal cancer xenograft tumors.

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Purity: 99.28%
CAS No.: 1262787-83-6
분자량:146.86 kDa

보관:

Please store the product under the recommended conditions in the Certificate of Analysis.

Patritumab Chemical Structure

CAS No.: 1262787-83-6

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Top Publications Citing

Use of MCE Products

Antibody-Drug Conjugates

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- 제품 설명
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- 신청
- Verified Bioactivity
Chemical Information
Publications (2)
순도&문서
References
Calculators
Help & FAQs
Bioz

Biological Activity

제품 설명

Isotype

Human IgG1(K214R) kappa

Recommend Isotype Controls

Human IgG1 (K214R) kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

ERBB3/HER3

All EGFR Isoforms :

All Akt Isoforms :

All ERK Isoforms :

All PARP Isoforms :

In Vitro

Patritumab targets to the extracellular domain (ECD) of HER3 and (10 μg/mL; 5 d) induces DiFi-HRG4 cells apoptosis^[1].
Patritumab (10 μg/mL; 6 h) markedly inhibits the phosphorylation of HER3 and AKT, without affecting that of ERK, in DiFi-HRG4 cells^[1].
Patritumab (10 μg/mL; 48 h) also induces the cleavage of PARP accompanied with both up-regulation of BIM and down-regulation of survivin expression^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

DiFi-HRG cells
Concentration:

10 μg/mL
Incubation Time:

6 hours
Result:

Inhibited the phosphorylation of HER3 and AKT as well as down-regulated survivin expression.

In Vivo

Patritumab (1 mg/mouse; i.p.; twice a week for 4 weeks) combines with 1 mg Cetuximab and restores Cetuximab sensitivity in DiFi-HRG tumor xenografts model in mice^[1].
Heregulin produced by colorectal cancer tumors harboring wild-type KRAS induces Cetuximab resistance, and that combination therapy with cetuximab and patritumab overcomes such resistance in vivo^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Female athymic nude mice (BALB/c; 5-6 weeks old) with DiFi-Mock1 or DiFi-HRG4 (s.c.)^[1]
Dosage:

1 mg/body
Administration:

Intraperitoneal injection; twice a week for 4 weeks
Result:

Individual Patritumab treatment had little effect on the growth of tumors formed by either cell line.
Combination of Cetuximab and Patritumab induced substantial regression of DiFi-HRG4 xenografts.

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Gene ID

2065 [NCBI]

Accession

P21860

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Format

Human IgG1 kappa

신청

ELISA, FACS, Functional assay

Verified Bioactivity

Flow cytometric analysis of 1×10⁶ MCF-7 cells labelling HER3 (red) with Patritumab (HY-P99275). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1(K214R) kappa Isotype Control (HY-P99995, blue) was used as the isotype control.
Immobilized Human HER3/ERBB3 Protein (ECD, His Tag) can bind Patritumab. The EC₅₀ for this effect is 1.41 ng/mL.
Loaded Patritumab on AHC2 biosensor, can bind HER3 Protein, Human (HEK293, His, HY-P72625) with an affinity constant of 1.804E-09 M as determined in BLI assay.

Chemical Information

CAS No. 1262787-83-6
Appearance Liquid
분자량 146.86 kDa
Color Colorless to light yellow
SMILES

[Patritumab]
Synonyms

AMG-888; U3-1287
선적

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
보관

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (2)

All WB

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Journal Impact Factor

Clin Cancer Res

ERBB3 overexpression is enriched in diverse patient populations with castration-sensitive prostate cancer and is associated with a unique AR activity signature. [Abstract]

2024 Apr 15;30(8):1530-1543. PMID: 38306015

Patritumab purchased from MedChemExpress. Usage Cited in: Clin Cancer Res. 2024 Apr 15;30(8):1530-1543. [Abstract]

Patritumab (10 μM; 2 h) reduced pHER3 after NRG1 stimulation (100 ng/mL; 15 min) in CWR-R1 cells.
bioRxiv

2026 Jun 19.

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (261 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

References

[1]. Kawakami H, et al. The anti-HER3 antibody patritumab abrogates cetuximab resistance mediated by heregulin in colorectal cancer cells. Oncotarget. 2014 Dec 15;5(23):11847-56. [Content Brief]

Calculators

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

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Molecular Weight Molecular Weight

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

Patritumab

2 Cited Publications

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Biological Activity
- 제품 설명
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Format
- 신청
- Biological Activity
Chemical Information
Publications (2)
순도&문서
References

1/16

Human IgG1 kappa
1/16

Flow cytometric analysis of 1×10⁶ MCF-7 cells labelling HER3 (red) with Patritumab (HY-P99275). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then stained with the primary antibody at 1/200 dilution for an hour at 4℃. Alexa Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1(K214R) kappa Isotype Control (HY-P99995, blue) was used as the isotype control.
1/16

Immobilized Human HER3/ERBB3 Protein (ECD, His Tag) can bind Patritumab. The EC₅₀ for this effect is 1.41 ng/mL.
1/16

Loaded Patritumab on AHC2 biosensor, can bind HER3 Protein, Human (HEK293, His, HY-P72625) with an affinity constant of 1.804E-09 M as determined in BLI assay.
1/16

Patritumab purchased from MedChemExpress. Usage Cited in: Clin Cancer Res. 2024 Apr 15;30(8):1530-1543. [Abstract]

Customer Validation
Patritumab (10 μM; 2 h) reduced pHER3 after NRG1 stimulation (100 ng/mL; 15 min) in CWR-R1 cells.
1/16

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Patritumab

Antibody Inhibitor Library

Publications Citing Use of MedChemExpress (MCE) Patritumab

All EGFR Isoforms

All Akt Isoforms

All ERK Isoforms

All PARP Isoforms

Biological Activity

Chemical Information

Publications (2)

순도&문서

References

Calculators

Help & FAQs

Patritumab

Antibody Inhibitor Library

Publications Citing Use of MedChemExpress (MCE) Patritumab

All EGFR Isoforms

All Akt Isoforms

All ERK Isoforms

All PARP Isoforms

Biological Activity

Chemical Information

Publications (2)

순도&문서

References

Calculators

Help & FAQs

Patritumab Related Classifications

Cat. No.: HY-P99275