Antiproliferative agent-85
Antiproliferative agent-85 is a CDK1 (CDC2) modulator. Antiproliferative agent-85 promotes M-phase transition by reducing inhibitory phosphorylation, and increases cyclin B1 protein expression and intracellular accumulation as well as pro-apoptotic BAX protein expression, thereby inducing M-phase cell cycle arrest and apoptosis through the intrinsic mitochondrial apoptosis (Apoptosis) pathway. Antiproliferative agent-85 can be used for research on breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 2083647-81-6
- Formula: C26H38O4
- Molecular Weight:414.58
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
CDK1 |
Bax |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| MDA-MB-468 | IC50 |
7.05 μM
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Antiproliferative activity against human MDA-MB-468 triple-negative breast cancer cells determined from three independent experiments.
Antiproliferative activity against human MDA-MB-468 triple-negative breast cancer cells determined from three independent experiments.
|
42800619 |
| JIMT-1 | IC50 |
7.19 μM
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Antiproliferative activity against human JIMT-1 HER2-positive breast cancer cells determined from three independent experiments.
Antiproliferative activity against human JIMT-1 HER2-positive breast cancer cells determined from three independent experiments.
|
42800619 |
| SK-BR-3 | IC50 |
9.48 μM
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Antiproliferative activity against human SKBr3 HER2-positive breast cancer cells determined from three independent experiments.
Antiproliferative activity against human SKBr3 HER2-positive breast cancer cells determined from three independent experiments.
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42800619 |
| MCF-10A | CC50 |
20.13 μM
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Cytotoxicity against human MCF-10A normal breast epithelial cells determined from three independent experiments.
Cytotoxicity against human MCF-10A normal breast epithelial cells determined from three independent experiments.
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42800619 |
In Vitro
Antiproliferative agent-85 selectively inhibits the proliferation of MDA-MB-468, JIMT-1, and SKBr3 breast cancer cells with IC50 values of 7.05, 7.19, and 9.48 μM, respectively, and exhibits a CC50 of 20.13 μM in MCF-10A cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468 cells
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Concentration:3.5, 10, 15 μM
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Incubation Time:24 h
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Result:Induced early-stage apoptosis; the apoptosis rate increased dose-dependently compared with the DMSO control.
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Cell Line:MDA-MB-468 cells
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Concentration:3.5, 10, 15 μM
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Incubation Time:24 h
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Result:Cleaved-PARP increased dose-dependently.
BAX was upregulated.
CL-Caspase-8 showed a distinct band at 15 μM.
The BAX/BCL2 ratio increased sharply even at low concentrations.
- Western Blot
- RNA extraction experimental
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- Protocol for Cell Cycle
- Breast Cancer Modeling
- Cell Viability Determination by MTT Colorimetric Assay
- Protocol For Protein Expression And Purification
- Kinase activity and phosphorylation assays
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Apoptosis Solutions
- Protocol for Kinase activity and phosphorylation assays
Chemical Information
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CAS No. 2083647-81-6
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Molecular Weight 414.58
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Formula C26H38O4
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SMILES
OC1=C(C/C=C(C)/CC/C=C(C)/CC/C=C(C)/C)C(O)=CC(O)=C1C(CC(C)C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Antiproliferative agent-85 Related Classifications
HY-190671 Related Classifications
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Keywords
- Antiproliferative agent-85
- 2083647-81-6
- Antiproliferative agent85
- Antiproliferative agent 85
- CDK
- Bcl-2 Family
- Apoptosis
- BAX
- CDK1
- HER2-positive breast cancer
- M-phase cell cycle arrest
- M-phase transition
- apoptosis
- breast cancer
- cyclin B1
- intrinsic mitochondrial apoptotic pathway
- triple-negative breast cancer
- Inhibitor
- inhibitor
- inhibit