Cat. No.: HY-N1465

Aristolochic acid D

(Synonyms: Aristolochic acid-IVa)

Customer Review

Based on 1 Customer Validation

Aristolochic acid D (Aristolochic acid-IVa) is an orally active PDE2 (IC₅₀: 4.673 μM) and CDK2 (IC₅₀: 25 μM) inhibitor that can be isolated from Aristolochia indica L. Aristolochic acid D exhibits anti-inflammatory activity and is non-carcinogenic and non-nephrotoxic. Aristolochic acid D can be used in the research of inflammation and tumor-related diseases.

For research use only. We do not sell to patients.

Purity: 99.74%
CAS No.: 17413-38-6
Formula: C₁₇H₁₁NO₈
Molecular Weight:357.27

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

Aristolochic acid D Chemical Structure

CAS No.: 17413-38-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]^[5]^[6]

IL-6

PDE2

4.673 μM (IC₅₀)

CDK2

25 μM (IC₅₀)

All Phosphodiesterase (PDE) Isoforms :

All CDK Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Neutrophil	IC₅₀	5.78 μg/mL Compound: 5	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation after 5 mins Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced superoxide anion generation after 5 mins	[PMID: 21353775]
Neutrophil	IC₅₀	8.49 μg/mL Compound: 5	Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release after 5 mins Antiinflammatory activity in human neutrophils assessed as inhibition of fMLP/CB-induced elastase release after 5 mins	[PMID: 21353775]

In Vitro

Aristolochic acid D (0-300 μM; 48 h) exerts no significant cytotoxicity on LLC-PK₁ cells and fails to induce caspase-3/7 activation^[1].
Aristolochic acid D (50-100 μM; 8 h) significantly reduces the production of TNF-α and IL-6 in RAW 264.7 cells treated with LPS (HY-D1056A1)^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[2]

Cell Line:

RAW 264.7 cells treated LPS (HY-D1056A1)
Concentration:

50 and 100 μM
Incubation Time:

Pretreated with 2 h, then co-incubation for 6 h
Result:

Reduced the production of TNF-α and IL-6.

In Vivo

Aristolochic acid D (400-600 μg/ear; topical application; single dose) exerts an improving effect in the mouse model of ear edema^[2].
Aristolochic acid D (100 mg/kg; oral gavage; single dose) significantly reduces TNF-α luciferase activity in TNF-IRES-Luc mice treated with LPS (HY-D1056A1), exhibiting a rapid inhibitory effect on systemic inflammation but with a short duration^[2].
Aristolochic acid D (3-10 mg/kg; oral gavage; five times a week; from day 12 to day 42) possesses anti-inflammatory activity in the mouse model of chronic arthritis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male ICR mice aged 6-8 weeks old treated phorbol 12-myristate 13-acetate (HY-18739) to induce ear edema^[2]
Dosage:

400 and 600 μg/ear
Administration:

Topical application; single dose
Result:

Exhibitd topical anti-inflammatory activity.
Significantly reduced ear weight, inflammatory cell infiltration and edema.

Animal Model:

Male BALB/cAnSmoc-Zap70^{em(W163C)Smoc} (SKG) mice aged 6-8 weeks old treated Mannan (HY-W145667) (SKG model that spontaneously develops chronic arthritis closely resembling human Rheumatoid Arthritis)^[3]
Dosage:

3 and 10 mg/kg
Administration:

Oral gavage; five times a week; from day 12 to day 42
Result:

Alleviated mannan-accelerated arthritis symptoms, reducing inflammatory infiltration, improving bone microstructure, decreasing levels of inflammatory factors, and regulating macrophage polarization as well as restoring the Th17/Treg balance.

Chemical Information

CAS No. 17413-38-6
Appearance Solid
Molecular Weight 357.27
Formula C₁₇H₁₁NO₈
Color Yellow to orange
SMILES

O=C(C1=C2C([N+]([O-])=O)=CC3=C(OC)C=C(O)C=C3C2=C(OCO4)C4=C1)O
Synonyms

Aristolochic acid-IVa
Structure Classification
- Phenols
- Monophenols
- Ketones, Aldehydes, Acids
Initial Source
- Plants
- other families
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvent & Solubility

In Vitro:

DMSO : 25 mg/mL (69.98 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.7990 mL

13.9950 mL

27.9900 mL

5 mM

0.5598 mL

2.7990 mL

5.5980 mL

10 mM

0.2799 mL

1.3995 mL

2.7990 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

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Molecular Weight Molecular Weight

Dilution Calculator

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (283 KB)
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Balachandran P, et al. Structure activity relationships of aristolochic acid analogues: toxicity in cultured renal epithelial cells. Kidney Int. 2005 May;67(5):1797-805. [Content Brief]

[2]. Liu W, et al. Non-carcinogenic/non-nephrotoxic aristolochic acid IVa exhibited anti-inflammatory activities in mice. J Nat Med. 2023 Mar;77(2):251-261. [Content Brief]

[3]. Gao QW, et al. Aristolochic acid IVa ameliorates arthritis in SKG Mice by regulating macrophage polarization and Th17/Treg balance. Phytomedicine. 2025 Apr;139:156557. [Content Brief]

[4]. Kupchan SM, et al. The isolation and structural elucidation of novel derivatives of aristolochic acid from Aristolochia indica. J Org Chem. 1968 Oct;33(10):3735-8. [Content Brief]

[5]. Song, GQ, et al. Inhibitors of phosphodiesterase 2 activity. CN116898835A. 2023-10-20.

[6]. Hegde VR, et al. Semi-synthetic aristolactams--inhibitors of CDK2 enzyme. Bioorg Med Chem Lett. 2010 Feb 15;20(4):1384-7. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.7990 mL	13.9950 mL	27.9900 mL	69.9751 mL
	5 mM	0.5598 mL	2.7990 mL	5.5980 mL	13.9950 mL
	10 mM	0.2799 mL	1.3995 mL	2.7990 mL	6.9975 mL
	15 mM	0.1866 mL	0.9330 mL	1.8660 mL	4.6650 mL
	20 mM	0.1400 mL	0.6998 mL	1.3995 mL	3.4988 mL
	25 mM	0.1120 mL	0.5598 mL	1.1196 mL	2.7990 mL
	30 mM	0.0933 mL	0.4665 mL	0.9330 mL	2.3325 mL
	40 mM	0.0700 mL	0.3499 mL	0.6998 mL	1.7494 mL
	50 mM	0.0560 mL	0.2799 mL	0.5598 mL	1.3995 mL
	60 mM	0.0467 mL	0.2333 mL	0.4665 mL	1.1663 mL

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Aristolochic acid D

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All Phosphodiesterase (PDE) Isoforms

All CDK Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Aristolochic acid D Related Classifications

HY-N1465 Related Classifications