Art. -Nr.: HY-B0916

Propoxur

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Propoxur is a reversible, competitive, orally active AChE inhibitor that can cross the blood-brain barrier. Propoxur inhibits AChE activity to induce neurotoxicity, while promoting MMP-2 expression and enhancing tumor cell migration and invasion by inducing intracellular ROS generation and activating the ERK/Nrf2 signaling pathway. On the one hand, Propoxur inhibits AChE, leading to acetylcholine accumulation and causing neurological dysfunction; on the other hand, it promotes Nrf2 nuclear translocation through ROS-dependent ERK1/2 phosphorylation, and upregulates MMP-2 and other invasion-related proteins. Propoxur is also a carbamate insecticide used to combat turf, forestry, and household pests.

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Reinheit: 99.43%
CAS. Nr.: 114-26-1
Formel: C₁₁H₁₅NO₃
Molecular Weight:209.24

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Propoxur Chemical Structure

CAS. Nr.: 114-26-1

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
Chemical Information
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target

MMP-2

AChE

Alle Cholinesterase (ChE) Isoform-spezifische Produkte anzeigen :

AChE
BChE

Alle MMP Isoform-spezifische Produkte anzeigen :

Alle ERK Isoform-spezifische Produkte anzeigen :

In Vitro

Propoxur (0.01-100 μM; 48 h) promotes cell migration and invasion in human breast cancer MCF-7 and MDA-MB-231 cells in a concentration-dependent manner, enhances MMP-2 protein expression, increases intracellular ROS levels, and activates ERK1/2 phosphorylation and Nrf2 nuclear translocation^[1]. The intracellular regulatory activity of Propoxur could be reversed by PD98059 (HY-12028)^[1].
Propoxur (10 μg/mL; 24 h) induces lipid peroxidation in hamster ovary CHO-K1 cells, significantly increases MDA production, decreases GSH content and GSH/GSSG ratio, and activates GR, GPx, and GST antioxidant enzyme activities. BSO pretreatment further aggravated oxidative damage^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-231 cells
Concentration:

0, 0.01, 1, 100 μM
Incubation Time:

48 h
Result:

Unchanged cell viability across concentrations, and reduced migration/invasion.
Dose-dependent increases in migration and invasion in Wound healing and Transwell assays, with 110.8-406.0% higher invasion compared to control.

Western Blot Analysis^[1]

Cell Line:

Human breast cancer MCF-7 and MDA-MB-231 cells
Concentration:

0, 0.01, 1, 100 μM
Incubation Time:

48 h
Result:

Increased MMP-2 protein expression without changes in MMP-9.
Dose-dependently increased phosphorylated ERK1/2 and nuclear Nrf2 levels, and the effects reversed by PD98059 (HY-12028), which suppressed MMP-2 overexpression.

Immunofluorescence^[1]

Cell Line:

MCF-7 & MDA-MB-231cells
Concentration:

0, 0.01, 1, 100 μM
Incubation Time:

48 h
Result:

Resulted significant ROS accumulation in Flow cytometry and immunofluorescence.

In Vivo

Propoxur (8.51 mg/kg/day; oral; 30 days) induces oxidative stress in brain regions (cerebellum, cortex, hippocampus) in rats, manifestes by lipid peroxidation (increased MDA), protein carbonylation (increased PCC), decreases GSH levels and decreased activities of antioxidant enzymes (SOD, CAT, GSH-Px, GST), while inhibiting acetylcholinesterase (AChE) activity. Pretreatment with essential oil extracted from Nigella sativa significantly improves the above indicators^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague-Dawley rats (250-300 g) with propoxur-induced neurotoxicity model^[3]
Dosage:

8.51 mg/kg
Administration:

Oral gavage, daily for 30 days
Result:

Significantly decreased body weight and weights of cerebellum, cortex, and hippocampus by 24%, 24%, 44%, respectively. AChE activity was reduced by 50% in cerebellum, 39% in cortex, and 54% in hippocampus.
Significantly decreased activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and glutathione-S-transferase (GST).

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

CAS. Nr. 114-26-1
Appearance Solid
Molecular Weight 209.24
Formel C₁₁H₁₅NO₃
Color White to off-white
SMILES

CC(C)OC1=CC=CC=C1OC(NC)=O
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : ≥ 100 mg/mL (477.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

* "≥" means soluble, but saturation unknown.

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7792 mL

23.8960 mL

47.7920 mL

5 mM

0.9558 mL

4.7792 mL

9.5584 mL

10 mM

0.4779 mL

2.3896 mL

4.7792 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.08 mg/mL (9.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.08 mg/mL (9.94 mM); Suspended solution

This protocol yields a suspended solution of ≥ 2.08 mg/mL (saturation unknown). Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.08 mg/mL (9.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (623 KB)
- English - EN (623 KB)
- Français - FR (623 KB)
- Deutsch - DE (623 KB)
- Norwegian - NO (623 KB)
- Español - ES (623 KB)
- Swedish - SV (623 KB)
- Italian - IT (623 KB)
- Korean - KR (623 KB)
- Portuguese - PT (623 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Shi Y, et al. Propoxur enhances MMP-2 expression and the corresponding invasion of human breast cancer cells via the ERK/Nrf2 signaling pathway. Oncotarget. 2017 Jul 7;8(50):87107-87123. [Content Brief]

[2]. Maran E, et al. Effects of aldicarb and propoxur on cytotoxicity and lipid peroxidation in CHO-K1 cells. Food Chem Toxicol. 2010 Jun;48(6):1592-6. [Content Brief]

[3]. Mohamadin A M, et al. Protective effects of Nigella sativa oil on propoxur-induced toxicity and oxidative stress in rat brain regions[J]. Pesticide biochemistry and physiology, 2010, 98(1): 128-134.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.7792 mL	23.8960 mL	47.7920 mL	119.4800 mL
	5 mM	0.9558 mL	4.7792 mL	9.5584 mL	23.8960 mL
	10 mM	0.4779 mL	2.3896 mL	4.7792 mL	11.9480 mL
	15 mM	0.3186 mL	1.5931 mL	3.1861 mL	7.9653 mL
	20 mM	0.2390 mL	1.1948 mL	2.3896 mL	5.9740 mL
	25 mM	0.1912 mL	0.9558 mL	1.9117 mL	4.7792 mL
	30 mM	0.1593 mL	0.7965 mL	1.5931 mL	3.9827 mL
	40 mM	0.1195 mL	0.5974 mL	1.1948 mL	2.9870 mL
	50 mM	0.0956 mL	0.4779 mL	0.9558 mL	2.3896 mL
	60 mM	0.0797 mL	0.3983 mL	0.7965 mL	1.9913 mL
	80 mM	0.0597 mL	0.2987 mL	0.5974 mL	1.4935 mL
	100 mM	0.0478 mL	0.2390 mL	0.4779 mL	1.1948 mL

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Propoxur

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Mongolian Medicine Compound Library

Environmental Pollutant Compound Library

Lactylation Compound Library

Alle Cholinesterase (ChE) Isoform-spezifische Produkte anzeigen

Alle MMP Isoform-spezifische Produkte anzeigen

Alle ERK Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Propoxur Related Classifications

HY-B0916 Related Classifications