Art. -Nr.: HY-139426

ML-SI3

Name: ML-SI3
Brand: MedChemExpress
SKU: HY-139426
Price: 85.00 USD
Availability: InStock
Rating: 5 (10 reviews)

(Synonyms: ML-SI3 (cis/trans mix))

10 Cited Publications

Kundenbewertung

Based on 10 publication(s) in Google Scholar

ML-SI3 is a mixture of cis/trans ML-SI3 (HY-139426A), which is a TRPML1/2 channel inhibitor with IC₅₀s of 4.7 μM and 1.7 μM, respectively. ML-SI3 also inhibits lysosomal calcium efflux and blocks downstream TRPML1-mediated autophagy. The cis/trans ML-SI3 (HY-139426A) components of ML-SI3 are TRPML2 activators with EC₅₀s of 3.3 μM and 9.4 μM, respectively.

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Reinheit: 99.91%
CAS. Nr.: 891016-02-7
Formel: C₂₃H₃₁N₃O₃S
Molecular Weight:429.58

Speicherung:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

ML-SI3 Chemical Structure

CAS. Nr.: 891016-02-7

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biologische Aktivität
- Beschreibung
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (10)
Lösungsmittel & Löslichkeit
- In Vitro
- In Vivo
Reinheit & Dokumentation
Verweise
Help & FAQs
Classifications
Bioz

Biologische Aktivität

Beschreibung

IC₅₀ & Target^[1]

Schistosome

TRPML1

4.7 μM (IC₅₀)

TRPML2

1.7 μM (IC₅₀)

TRPML3

12.5 μM (IC₅₀)

Alle Parasite Isoform-spezifische Produkte anzeigen :

Alle TRP Channel Isoform-spezifische Produkte anzeigen :

In Vitro

ML-SI3 (10 μM) inhibits ML-SA1-evoked Ca²⁺ signals in HeLa cells^[3].
ML-SI3 (25-75 μM, 24h) disrupts tegumental integrity of adult schistosomes^[4].
ML-SI3 (10 μM) blocks Rapamycin (HY-10219)-evoked I_TRPML1 in mimic of lysosomal lumen^[5].
ML-SI3 (3 μM, 6 h) abolishes the increase in both LC3II and p62 levels induced by hypoxia/reoxygenation (H/R) (4 h H/2 h R) in neonatal rat ventricular myocytes (NRVM)^[6].

Table: Activation and inhibition of the three subtypes of TRPML by Others ML-SI3-type compounds^[2]

Product_Names	Catalog_No	EC₅₀ for activation [μM]			IC₅₀ for inhibition [μM]
Product_Names	Catalog_No	TRPML1	TRPML2	TRPML3	TRPML1	TRPML2	TRPML3
(rel)-ML-SI3	HY-139426A	-	3.3±1.5	-	3.1±1.5	-	28.5±15.9
(cis)-ML-SI3	/	-	9.4±2.7	29.0±4.1	18.5±1.9	-	-
(1S,2S)-ML-SI3	HY-134819	-	2.7±1.4	10.8±0.5	5.9±1.7	-	-
(1R,2R)-ML-SI3	HY-134819A	-	-	-	1.6±0.7	2.3±1.1	12.5±7.6
Mean values ± SD of at least three independent experiments are shown. -: no effect.
ML-SI3	HY-139426	The mixtrue of (rel)-ML-SI3 and (cis)-ML-SI3

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

ML-SI3 (1.5 mg/kg, i.p., four times) attenuates I/R injury in mouse cardiomyocytes^[6].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Myocardial Ischemia/reperfusion (I/R) injury mice^[5]
Dosage:

1.5 mg/kg
Administration:

Intraperitoneal injection (i.p.), four times before and during the in vivo I/R (ischemia 30 min and reperfusion 1 day)
Result:

Restored the blocked autophagic fux in the cardiomyocytes subjected to I/R.

Chemical Information

CAS. Nr. 891016-02-7
Appearance Solid
Molecular Weight 429.58
Formel C₂₃H₃₁N₃O₃S
Color White to off-white
SMILES

O=S(C1=CC=CC=C1)(NC2C(N3CCN(C4=CC=CC=C4OC)CC3)CCCC2)=O
Synonyms

ML-SI3 (cis/trans mix)
Versand

Room temperature in continental US; may vary elsewhere.

Speicherung

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Publications (10)

All Cell Imaging/StainingFlow CytometryWB

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Journal Impact Factor

Nat Cell Biol

Pressure sensing of lysosomes enables control of TFEB responses in macrophages. [Abstract]

2024 Aug;26(8):1247-1260. PMID: 38997458
J Mater Sci Technol

2026 May 9;277:252-264.
Adv Sci (Weinh)

Nortriptyline Inhibits Lysosomal Exocytosis-Mediated SASP During Gastric Cancer Progression via Targeting HOXA1-PITX2 Phase Separation. [Abstract]

2025 Sep 25:e12407. PMID: 40995693

ML-SI3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 25:e12407. [Abstract]

Under SD condition, ML-SI3 (10 μM) reversed the significant increases or decreases in intracellular calcium concentration, lysosome number, or anterograde transport distance caused by persistent overexpression or silencing of PITX2.

ML-SI3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 25:e12407. [Abstract]

Representative Lyso-Tracker Red staining images showed the number and anterograde transport distance of lysosomes in AGS and HGC-27 cells stably transfected with mock, PITX2, sh-Scb, sh-PITX2 #1, or sh-PITX2 #2 under SD conditions and those treated with ML-SI3 (10 μM).

ML-SI3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 25:e12407. [Abstract]

Western blot assay indicated the levels of PITX2, LC3, and SQSTM1 in AGS and HGC-27 cells stably transfected with mock, PITX2, sh-Scb, or sh-PITX2 #1 under SD conditions, and those treated with ML-SI3 (10 μM).

ML-SI3 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2025 Sep 25:e12407. [Abstract]

Representative images and quantification of autophagic flux reporters RFP- and GFP-LC3 (arrowheads) in AGS and HGC-27 cells stably transfected with empty vector (mock), PITX2, scramble shRNA (sh-Scb), or sh-PITX2 #1 under SD condition, and those treated with ML-SI3 (10 μM) were presented.
Cell Death Dis

Lysosomal exocytosis by macrophages as a druggable mechanism for anti-inflammatory clearance of dead adipocytes in adipose tissue. [Abstract]

2025 Dec 23. PMID: 41436477
J Immunother Cancer

Macrophage NLRP3 activation and IL-1β release drive osimertinib-induced antitumor immunity. [Abstract]

2025 Oct 5;13(10):e012182. PMID: 41052880

ML-SI3 purchased from MedChemExpress. Usage Cited in: J Immunother Cancer. 2025 Oct 5;13(10):e012182. [Abstract]

Flow cytometry analysis of Fluo-4 AM (5 μM) staining in BMDM cells after osimertinib stimulation with or without ML-SI3 (5 μM) pretreatment. ML-SI3 inhibited Osimertinib-induced Ca2+ accumulation.
Cell Signal

HDAC inhibitor enhances ferroptosis susceptibility of AML cells by stimulating iron metabolism. [Abstract]

2025 Mar:127:111583. PMID: 39756501
FASEB J

TRPML1 in Cisplatin-Induced Acute Kidney Injury: A New Target for Renal Tubular Epithelial Protection by Regulating Lysosomal Calcium Homeostasis. [Abstract]

2026 Jan 15;40(1):e71421. PMID: 41499332
Cell Mol Bioeng

Hyperosmotic Stress Promotes the Nuclear Translocation of TFEB in Tubular Epithelial Cells Depending on Intracellular Ca2+ Signals via TRPML Channels. [Abstract]

2025 Jan 21;18(1):39-52. PMID: 39949488
Dresden University of Technology

2026.
bioRxiv

2025 March 19.

Lösungsmittel & Löslichkeit

In Vitro:

DMSO : 50 mg/mL (116.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3279 mL

11.6393 mL

23.2786 mL

5 mM

0.4656 mL

2.3279 mL

4.6557 mL

10 mM

0.2328 mL

1.1639 mL

2.3279 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molaritätsrechner

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Konzentration Konzentration

Volumen Volumen

Molecular Weight Molecular Weight

Verdünnungsrechner

Konzentration _{(Stammlösung)} × Volumen _{(Stammlösung)} = Konzentration _{(Ziellösung)} × Volumen _{(Ziellösung)}

Konzentration _{(Stammlösung)} Konzentration _{(Stammlösung)}

Volumen _{(Stammlösung)} Volumen _{(Stammlösung)}

Konzentration _{(Ziellösung)} Konzentration _{(Ziellösung)}

Volumen _{(Ziellösung)} Volumen _{(Ziellösung)}

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (5.82 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Reinheit & Dokumentation

Select Batch:

Reinheit: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

Verweise

[1]. Rühl P, et al. Estradiol analogs attenuate autophagy, cell migration and invasion by direct and selective inhibition of TRPML1, independent of estrogen receptors. Sci Rep. 2021 Apr 15;11(1):8313. [Content Brief]

[2]. Leser C, et al. Chemical and pharmacological characterization of the TRPML calcium channel blockers ML-SI1 and ML-SI3. Eur J Med Chem. 2021 Jan 15;210:112966. [Content Brief]

[3]. Kilpatrick BS, et al. Endo-lysosomal TRP mucolipin-1 channels trigger global ER Ca2+ release and Ca2+ influx. J Cell Sci. 2016 Oct 15;129(20):3859-3867. [Content Brief]

[4]. Bais S, et al. Schistosome TRPML channels play a role in neuromuscular activity and tegumental integrity. Biochimie. 2022 Mar;194:108-117. [Content Brief]

[5]. Zhang X, et al. Rapamycin directly activates lysosomal mucolipin TRP channels independent of mTOR. PLoS Biol. 2019 May 21;17(5):e3000252. [Content Brief]

[6]. Xing Y, et al. Blunting TRPML1 channels protects myocardial ischemia/reperfusion injury by restoring impaired cardiomyocyte autophagy. Basic Res Cardiol. 2022 Apr 7;117(1):20. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3279 mL	11.6393 mL	23.2786 mL	58.1964 mL
	5 mM	0.4656 mL	2.3279 mL	4.6557 mL	11.6393 mL
	10 mM	0.2328 mL	1.1639 mL	2.3279 mL	5.8196 mL
	15 mM	0.1552 mL	0.7760 mL	1.5519 mL	3.8798 mL
	20 mM	0.1164 mL	0.5820 mL	1.1639 mL	2.9098 mL
	25 mM	0.0931 mL	0.4656 mL	0.9311 mL	2.3279 mL
	30 mM	0.0776 mL	0.3880 mL	0.7760 mL	1.9399 mL
	40 mM	0.0582 mL	0.2910 mL	0.5820 mL	1.4549 mL
	50 mM	0.0466 mL	0.2328 mL	0.4656 mL	1.1639 mL
	60 mM	0.0388 mL	0.1940 mL	0.3880 mL	0.9699 mL
	80 mM	0.0291 mL	0.1455 mL	0.2910 mL	0.7275 mL
	100 mM	0.0233 mL	0.1164 mL	0.2328 mL	0.5820 mL

Bioz

Keywords

ML-SI3

10 Cited Publications

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ML-SI3

ML-SA1

ML-SA5

AC1903

SKF-96365 (hydrochloride)

ML204

TRPM4-IN-1

ML-SI1

Adenosine 5′-diphosphoribose sodium

MitoSOX Red

Bafilomycin A1

Paclitaxel

BAPTA-AM

Publications Citing Use of MedChemExpress (MCE) ML-SI3

Alle Parasite Isoform-spezifische Produkte anzeigen

Alle TRP Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

Bioz

ML-SI3

ML-SA1

ML-SA5

AC1903

SKF-96365 (hydrochloride)

ML204

TRPM4-IN-1

ML-SI1

Adenosine 5′-diphosphoribose sodium

MitoSOX Red

Bafilomycin A1

Paclitaxel

BAPTA-AM

Publications Citing Use of MedChemExpress (MCE) ML-SI3

Alle Parasite Isoform-spezifische Produkte anzeigen

Alle TRP Channel Isoform-spezifische Produkte anzeigen

Biologische Aktivität

Chemical Information

Publications (10)

Lösungsmittel & Löslichkeit

Reinheit & Dokumentation

Verweise

Help & FAQs

Complete Stock Solution Preparation Table

ML-SI3 Related Classifications

HY-139426 Related Classifications

Bioz