Nodosin
Based on 1 Customer Validation
Nodosin is an orally active diterpenoid compound that can be isolated from Isodon serra. Nodosin can inhibit the proliferation and induce cell cycle arrest and apoptosis of tumor cells. Nodosin can also inhibit oxidative stress, inflammatory responses, and ferroptosis. Nodosin has anti-inflammatory and anti-tumor activities.
Nos produits utilisent uniquement pour la recherche. Nous ne vendons pas aux patients.
- Pureté: 98.61%
- CAS No.: 10391-09-0
- Formule: C20H26O6
- Masse moléculaire:362.42
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Stockage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
Activité biologique
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
>10 μM
Compound: 16
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Cytotoxicity against human A549 cells after 48 hrs
Cytotoxicity against human A549 cells after 48 hrs
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[PMID: 19697926] |
| A549 | IC50 |
>40 μM
Compound: 9
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Cytotoxicity against human A549 cells after 48 hrs by MTS assay
Cytotoxicity against human A549 cells after 48 hrs by MTS assay
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[PMID: 24219809] |
| HepG2 | IC50 |
1.92 μM
Compound: 16
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Cytotoxicity against human HepG2 cells after 48 hrs
Cytotoxicity against human HepG2 cells after 48 hrs
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[PMID: 19697926] |
| HL-60 | IC50 |
15 μM
Compound: 9
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Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
Cytotoxicity against human HL60 cells after 48 hrs by MTS assay
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[PMID: 24219809] |
| K562 | IC50 |
0.54 μM
Compound: 16
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Cytotoxicity against human K562 cells after 48 hrs
Cytotoxicity against human K562 cells after 48 hrs
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[PMID: 19697926] |
| MCF7 | IC50 |
23.5 μM
Compound: 9
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Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
Cytotoxicity against human MCF7 cells after 48 hrs by MTS assay
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[PMID: 24219809] |
| RAW264.7 | IC50 |
18.4 μM
Compound: 9
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Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay
Inhibition of LPS-stimulated NO production in mouse RAW264.7 cells after 18 hrs by Griess assay
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[PMID: 24219809] |
| SMMC-7721 | IC50 |
27 μM
Compound: 9
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Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
Cytotoxicity against human SMMC7721 cells after 48 hrs by MTS assay
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[PMID: 24219809] |
| SW480 | IC50 |
12.1 μM
Compound: 9
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Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
Cytotoxicity against human SW480 cells after 48 hrs by MTS assay
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[PMID: 24219809] |
| SW780 | IC50 |
9.99 μM
Compound: Nodosin
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Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay
Cytotoxicity against human SW780 cells assessed as reduction in cell growth by CCK8 assay
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[PMID: 35976233] |
| T-24 | IC50 |
4.91 μM
Compound: Nodosin
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Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay
Cytotoxicity against human T24 cells assessed as reduction in cell growth by CCK8 assay
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[PMID: 35976233] |
| UMUC3 | IC50 |
3.45 μM
Compound: Nodosin
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Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay
Cytotoxicity against human UMUC3 cells assessed as reduction in cell growth by CCK8 assay
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[PMID: 35976233] |
Nodosin (0-30 μM; 18-72 h) can inhibit the growth of multiple tumor cell lines (the IC50 values for SNU638, SK-HEP-1, A549, HCT116, and MDA-MB-231 are 7.53, 5.33, 13.73, 4.05, and 7.12 μM, respectively), and induce cell cycle arrest and apoptosis in HCT116 cells. The mechanism involves the inhibition of the Wnt/β-catenin signaling pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HCT116 cells
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Concentration:0, 7.5, 15 and 30 μM
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Incubation Time:18 h
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Result:Significantly suppressed Wnt/β-catenin target protein expressions including β-catenin, p-GSK-3β, c-Myc, and Survivin.
Nodosin (100 μg/mL; tail vein injection; single dose) has a protective effect in a rat liver transplantation model. It can reduce the pathological damage of rat liver tissue, decrease the number of apoptotic hepatocytes, and promote hepatocyte proliferation[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male Sprague-Dawley rats (180±20 g, 8 weeks old) treated LPS (HY-D1056) to establish the acute kidney injury model[2]
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Dosage:2 and 4 mg/kg
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Administration:Oral administration; 7 days
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Result:Significantly decreased Serum Scr, BUN, urine NGAL, and KIM-1 levels.
Decreased the levels of kidney MDA and 4-HNE, increased the levels of antioxidant enzymes (SOD, CAT, GPx, GST) and Nrf2, NQO1, HO-1, and reduced Keap-1 mRNA levels.
Reversed excessive ferroptosis in the kidneys, as indicated by increased mRNA and protein levels of GPX4, SLC7A11, and FTH-1.
Significantly reduced the levels of inflammatory markers (TLR4, MYD88, NF-κB p65, IKKβ, IL-1β) and increased IL-10 levels.
Seduced Histopathological changes in the kidneys.
Chemical Information
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CAS No. 10391-09-0
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Appearance Solid
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Masse moléculaire 362.42
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Formule C20H26O6
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Color White to off-white
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SMILES
O[C@H]1[C@@]([C@]2(C(C3=C)=O)C[C@H]3C1)([H])[C@@]([C@@](CC4)([H])OC2=O)(CO5)[C@](C4(C)C)([H])[C@@H]5O
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvant et solubilité
DMSO : 125 mg/mL (344.90 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (5.74 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (289 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Bae ES, et al. Anti-Proliferative Activity of Nodosin, a Diterpenoid from Isodon serra, via Regulation of Wnt/β-Catenin Signaling Pathways in Human Colon Cancer Cells. Biomol Ther (Seoul). 2020 Sep 1;28(5):465-472. [Content Brief]
[2]. Su C, et al. Protective effects of nodosin against lipopolysaccharide-induced acute kidney injury through regulation of oxidative stress, inflammation, and ferroptosis in rats. Naunyn Schmiedebergs Arch Pharmacol. 2024 Oct;397(10):8009-8022 [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.7592 mL | 13.7961 mL | 27.5923 mL | 68.9807 mL |
| 5 mM | 0.5518 mL | 2.7592 mL | 5.5185 mL | 13.7961 mL | |
| 10 mM | 0.2759 mL | 1.3796 mL | 2.7592 mL | 6.8981 mL | |
| 15 mM | 0.1839 mL | 0.9197 mL | 1.8395 mL | 4.5987 mL | |
| 20 mM | 0.1380 mL | 0.6898 mL | 1.3796 mL | 3.4490 mL | |
| 25 mM | 0.1104 mL | 0.5518 mL | 1.1037 mL | 2.7592 mL | |
| 30 mM | 0.0920 mL | 0.4599 mL | 0.9197 mL | 2.2994 mL | |
| 40 mM | 0.0690 mL | 0.3449 mL | 0.6898 mL | 1.7245 mL | |
| 50 mM | 0.0552 mL | 0.2759 mL | 0.5518 mL | 1.3796 mL | |
| 60 mM | 0.0460 mL | 0.2299 mL | 0.4599 mL | 1.1497 mL | |
| 80 mM | 0.0345 mL | 0.1725 mL | 0.3449 mL | 0.8623 mL | |
| 100 mM | 0.0276 mL | 0.1380 mL | 0.2759 mL | 0.6898 mL |
Nodosin Related Classifications
HY-N3181 Related Classifications
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