Cat. No.: HY-N7005

Clitorin

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Clitorin is an orally active flavonoid compound that can be isolated from Carica papaya. Clitorin is an inhibitor of EGFR (IC₅₀: 89.58 nM) and aromatase (IC₅₀: 77.41 nM). Clitorin has antioxidant and anti-tumor activities. In the DPPH and ABTS radical scavenging assays, Clitorin has IC₅₀ values of 91.96 ppm and 250.45 ppm, respectively. In addition, Clitorin can regulate lipogenesis and fatty acid oxidation and can be used in the research of non-alcoholic fatty liver disease.

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Pureté: 99.45%
CAS No.: 55804-74-5
Formule: C₃₃H₄₀O₁₉
Masse moléculaire:740.66

Stockage:

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Clitorin Chemical Structure

CAS No.: 55804-74-5

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Activité biologique
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]^[2]

PPARα

Aromatase

77.41 nM (IC₅₀)

Voir tous les produits spécifiques à Isoform EGFR :

Voir tous les produits spécifiques à Isoform Cytochrome P450 :

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In Vitro

Clitorin (24 h) is toxic to MCF-7 breast cancer cells, with an IC₅₀ of 2.47 μM^[1].
Clitorin (50-200 μM; 48 h) can reduce lipid accumulation, decrease the contents of triglycerides and total cholesterol, inhibit the mRNA levels of lipogenic genes, and enhance the mRNA levels of fatty acid oxidation genes in Oleic acid (HY-N1446)-stimulated HepG2 cells^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Real Time qPCR^[2]

Cell Line:

Oleic acid (HY-N1446)-stimulated HepG2 cells
Concentration:

50, 100 and 200 μM
Incubation Time:

48 h
Result:

Inhibited the mRNA levels of SREBP1, ACC, FAS and HMGCR.
Enhanced the mRNA levels of CPT-1, PPARα, and AMPK.

In Vivo

Clitorin (10-20 mg/kg; oral administration; 4 weeks) has an improving effect in a mouse model of hepatic steatosis^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male C57BL/6J mice aged 6 weeks old treated western diet (WD) to induce hepatic steatosis^[2]
Dosage:

10 and 20 mg/kg
Administration:

Oral administration (p.o.); 4 weeks
Result:

Sgnificantly alleviated body weight gain and hepatic steatosis features (NAFLD activity score, micro-, and macro-vesicular steatosis) in WD-induced hepatic steatosis mice.
Significantly decreased protein expressions of SREBP1, PPARγ and C/EBPα in WD-induced hepatic steatosis mice.
Significantly diminished the mRNA levels of SREBP1, ACC, FAS, and HMGCR and enhanced the mRNA levels of PPARαand CTP-1, as well as AMPK in the liver of WD-induced hepatic steatosis mice.

Chemical Information

CAS No. 55804-74-5
Appearance Solid
Masse moléculaire 740.66
Formule C₃₃H₄₀O₁₉
Color Light yellow to yellow
SMILES

O=C1C(O[C@H]2[C@@H]([C@H]([C@H](O)[C@@H](CO[C@H]3[C@@H]([C@@H]([C@@H](O)[C@H](C)O3)O)O)O2)O)O[C@@]4([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O4)O)O)=C(C5=CC=C(O)C=C5)OC6=CC(O)=CC(O)=C61
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
- Plants
- other families
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, sealed storage, away from moisture and light

* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (135.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3501 mL

6.7507 mL

13.5015 mL

5 mM

0.2700 mL

1.3501 mL

2.7003 mL

10 mM

0.1350 mL

0.6751 mL

1.3501 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: 2.5 mg/mL (3.38 mM); Suspended solution; Need ultrasonic

This protocol yields a suspended solution of 2.5 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (285 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Hamed ANE, et al. Chemical constituents from Carica papaya Linn. leaves as potential cytotoxic, EGFRwt and aromatase (CYP19A) inhibitors; a study supported by molecular docking. RSC Adv. 2022 Mar 23;12(15):9154-9162. [Content Brief]

[2]. Cominguez DC, et al. Clitorin ameliorates western diet-induced hepatic steatosis by regulating lipogenesis and fatty acid oxidation in vivo and in vitro. Sci Rep. 2022 Mar 9;12(1):4154. [Content Brief]

[3]. Teh B P, et al. Clitorin and manghaslin from Carica papaya leaf juice: characterisation and antioxidant properties. 2023.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3501 mL	6.7507 mL	13.5015 mL	33.7537 mL
	5 mM	0.2700 mL	1.3501 mL	2.7003 mL	6.7507 mL
	10 mM	0.1350 mL	0.6751 mL	1.3501 mL	3.3754 mL
	15 mM	0.0900 mL	0.4500 mL	0.9001 mL	2.2502 mL
	20 mM	0.0675 mL	0.3375 mL	0.6751 mL	1.6877 mL
	25 mM	0.0540 mL	0.2700 mL	0.5401 mL	1.3501 mL
	30 mM	0.0450 mL	0.2250 mL	0.4500 mL	1.1251 mL
	40 mM	0.0338 mL	0.1688 mL	0.3375 mL	0.8438 mL
	50 mM	0.0270 mL	0.1350 mL	0.2700 mL	0.6751 mL
	60 mM	0.0225 mL	0.1125 mL	0.2250 mL	0.5626 mL
	80 mM	0.0169 mL	0.0844 mL	0.1688 mL	0.4219 mL
	100 mM	0.0135 mL	0.0675 mL	0.1350 mL	0.3375 mL

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Clitorin

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Voir tous les produits spécifiques à Isoform EGFR

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Activité biologique

Chemical Information

Solvant et solubilité