Clitorin
Based on 1 Customer Validation
Clitorin is an orally active flavonoid compound that can be isolated from Carica papaya. Clitorin is an inhibitor of EGFR (IC50: 89.58 nM) and aromatase (IC50: 77.41 nM). Clitorin has antioxidant and anti-tumor activities. In the DPPH and ABTS radical scavenging assays, Clitorin has IC50 values of 91.96 ppm and 250.45 ppm, respectively. In addition, Clitorin can regulate lipogenesis and fatty acid oxidation and can be used in the research of non-alcoholic fatty liver disease.
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- Pureté: 99.45%
- CAS No.: 55804-74-5
- Formule: C33H40O19
- Masse moléculaire:740.66
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Stockage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Pureté et documentation
- Références
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Help & FAQs
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Activité biologique
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PPARα |
Aromatase 77.41 nM (IC50) |
Clitorin (24 h) is toxic to MCF-7 breast cancer cells, with an IC50 of 2.47 μM[1].
Clitorin (50-200 μM; 48 h) can reduce lipid accumulation, decrease the contents of triglycerides and total cholesterol, inhibit the mRNA levels of lipogenic genes, and enhance the mRNA levels of fatty acid oxidation genes in Oleic acid (HY-N1446)-stimulated HepG2 cells[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:Oleic acid (HY-N1446)-stimulated HepG2 cells
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Concentration:50, 100 and 200 μM
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Incubation Time:48 h
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Result:Inhibited the mRNA levels of SREBP1, ACC, FAS and HMGCR.
Enhanced the mRNA levels of CPT-1, PPARα, and AMPK.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Male C57BL/6J mice aged 6 weeks old treated western diet (WD) to induce hepatic steatosis[2]
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Dosage:10 and 20 mg/kg
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Administration:Oral administration (p.o.); 4 weeks
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Result:Sgnificantly alleviated body weight gain and hepatic steatosis features (NAFLD activity score, micro-, and macro-vesicular steatosis) in WD-induced hepatic steatosis mice.
Significantly decreased protein expressions of SREBP1, PPARγ and C/EBPα in WD-induced hepatic steatosis mice.
Significantly diminished the mRNA levels of SREBP1, ACC, FAS, and HMGCR and enhanced the mRNA levels of PPARαand CTP-1, as well as AMPK in the liver of WD-induced hepatic steatosis mice.
Chemical Information
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CAS No. 55804-74-5
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Appearance Solid
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Masse moléculaire 740.66
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Formule C33H40O19
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Color Light yellow to yellow
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SMILES
O=C1C(O[C@H]2[C@@H]([C@H]([C@H](O)[C@@H](CO[C@H]3[C@@H]([C@@H]([C@@H](O)[C@H](C)O3)O)O)O2)O)O[C@@]4([H])[C@@H]([C@@H]([C@@H](O)[C@H](C)O4)O)O)=C(C5=CC=C(O)C=C5)OC6=CC(O)=CC(O)=C61
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Structure Classification
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Initial Source
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvant et solubilité
DMSO : 100 mg/mL (135.01 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (3.38 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureté et documentation
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Fiche technique (285 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Instruction de manipulation (2659 KB)
Références
[1]. Hamed ANE, et al. Chemical constituents from Carica papaya Linn. leaves as potential cytotoxic, EGFRwt and aromatase (CYP19A) inhibitors; a study supported by molecular docking. RSC Adv. 2022 Mar 23;12(15):9154-9162. [Content Brief]
[2]. Cominguez DC, et al. Clitorin ameliorates western diet-induced hepatic steatosis by regulating lipogenesis and fatty acid oxidation in vivo and in vitro. Sci Rep. 2022 Mar 9;12(1):4154. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3501 mL | 6.7507 mL | 13.5015 mL | 33.7537 mL |
| 5 mM | 0.2700 mL | 1.3501 mL | 2.7003 mL | 6.7507 mL | |
| 10 mM | 0.1350 mL | 0.6751 mL | 1.3501 mL | 3.3754 mL | |
| 15 mM | 0.0900 mL | 0.4500 mL | 0.9001 mL | 2.2502 mL | |
| 20 mM | 0.0675 mL | 0.3375 mL | 0.6751 mL | 1.6877 mL | |
| 25 mM | 0.0540 mL | 0.2700 mL | 0.5401 mL | 1.3501 mL | |
| 30 mM | 0.0450 mL | 0.2250 mL | 0.4500 mL | 1.1251 mL | |
| 40 mM | 0.0338 mL | 0.1688 mL | 0.3375 mL | 0.8438 mL | |
| 50 mM | 0.0270 mL | 0.1350 mL | 0.2700 mL | 0.6751 mL | |
| 60 mM | 0.0225 mL | 0.1125 mL | 0.2250 mL | 0.5626 mL | |
| 80 mM | 0.0169 mL | 0.0844 mL | 0.1688 mL | 0.4219 mL | |
| 100 mM | 0.0135 mL | 0.0675 mL | 0.1350 mL | 0.3375 mL |
Clitorin Related Classifications
HY-N7005 Related Classifications
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