Cat. No.: HY-N7064

Iminostilbene

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Iminostilbene is a chemical precursor of carbamazepine. Additionally, Iminostilbene is an orally active inhibitor of PKM2 (Pyruvate Kinase M2) and COX2 (Cyclooxygenase-2). Iminostilbene exerts its effects by inhibiting PKM2 and its interaction with HIF-1α and STAT3, reducing COX2 and iNOS expression, and decreasing LPS-induced release of IL-1β, IL-6, TNF-α, and MCP-1, thereby suppressing macrophage-mediated inflammatory responses and improving myocardial ischemia/reperfusion (MI/R) injury. Iminostilbene holds promise for research in inflammation regulation, cardiovascular diseases (such as MI/R injury), and macrophage-mediated immune-related diseases.

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Pureté: 99.66%
CAS No.: 256-96-2
Formule: C₁₄H₁₁N
Masse moléculaire:193.25

Stockage:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Iminostilbene Chemical Structure

CAS No.: 256-96-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target

COX-2

iNOS

STAT3

HIF-1α

Voir tous les produits spécifiques à Isoform TNF Receptor :

Voir tous les produits spécifiques à Isoform HIF/HIF Prolyl-Hydroxylase :

Voir tous les produits spécifiques à Isoform STAT :

Voir tous les produits spécifiques à Isoform Interleukin Related :

Voir tous les produits spécifiques à Isoform COX :

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Cellular Effect

Cell Line	Type	Value	Description	References
U-937	IC₅₀	2.58 μM Compound: 11	Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay Cytotoxicity in UV-irradiated human U937 cells exposed to photosensitizer for 2 hrs before irradiation for 20 mins by WST-1 assay	[PMID: 24900465]

In Vitro

Iminostilbene (2.5-10 μM, 30 h) inhibits LPS-induced inflammatory response, reduces PKM2 expression, and suppresses its downstream signaling pathways in RAW264.7 macrophages^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[2]

Cell Line:

LPS (HY-D1056)-induced RAW264.7 macrophages (mouse-derived)
Concentration:

2.5 μM, 5 μM, 10 μM
Incubation Time:

30 h (6 h pre-treatment with Iminostilben, followed by LPS stimulation for 24 h)
Result:

Showed no significant effect on cell viability at low doses, slight reduction at high dose (10 μM).

Real Time qPCR^[2]

Cell Line:

LPS (HY-D1056)-induced RAW264.7 macrophages (mouse-derived)
Concentration:

2.5 μM, 5 μM, 10 μM
Incubation Time:

30 h (6 h pre-treatment with Iminostilben, followed by LPS stimulation for 24 h)
Result:

Suppressed IL-1β, IL-6, MCP-1, and TNF-α mRNA levels.

Western Blot Analysis^[2]

Cell Line:

LPS (HY-D1056)-induced RAW264.7 macrophages (mouse-derived)
Concentration:

2.5 μM, 5 μM, 10 μM
Incubation Time:

30 h (6 h pre-treatment with Iminostilben, followed by LPS stimulation for 24 h)
Result:

Inhibited COX2, PKM2, and iNOS protein expression and reduced PKM2-HIF-1α-STAT3 interaction.

In Vivo

Iminostilbene (0.625 mg/kg, 1.25 mg/kg, p.o., once daily for 5 days) improved cardiac function and suppressed inflammation in the myocardial ischemia/reperfusion (MI/R) injury SD rat model^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Myocardial ischemia/reperfusion (MI/R) injury Sprague-Dawley (SD) rat model^[2]
Dosage:

0.625 mg/kg, 1.25 mg/kg
Administration:

Oral gavage (p.o.), once daily for 5 days
Result:

Improved cardiac function, reduced myocardial infarction area.
Decreased CK-MB, LDH, and cTnT levels.
Reduced inflammatory cell infiltration, inhibited COX2 and iNOS expression, and suppressed IL-1β and IL-6 RNA and protein levels.

Chemical Information

CAS No. 256-96-2
Appearance Solid
Masse moléculaire 193.25
Formule C₁₄H₁₁N
Color Yellow to orange
SMILES

C12=CC=CC=C1C=CC3=CC=CC=C3N2
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Solvant et solubilité

In Vitro:

DMSO : 50 mg/mL (258.73 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.1746 mL

25.8732 mL

51.7464 mL

5 mM

1.0349 mL

5.1746 mL

10.3493 mL

10 mM

0.5175 mL

2.5873 mL

5.1746 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (12.94 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (282 KB)
SDS (597 KB)
- English - EN (597 KB)
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- Deutsch - DE (597 KB)
- Norwegian - NO (597 KB)
- Español - ES (597 KB)
- Swedish - SV (597 KB)
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- Portuguese - PT (597 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Christian P, et al. Crystallization of Carbamazepine in Proximity to Its Precursor Iminostilbene and a Silica Surface. Cryst Growth Des. 2016 May 4;16(5):2771-2778. Epub 2016 Mar 30. [Content Brief]

[2]. Lu S, et al. Iminostilbene, a novel small-molecule modulator of PKM2, suppresses macrophage inflammation in myocardial ischemia-reperfusion injury. J Adv Res. 2020 Sep 9;29:83-94. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.1746 mL	25.8732 mL	51.7464 mL	129.3661 mL
	5 mM	1.0349 mL	5.1746 mL	10.3493 mL	25.8732 mL
	10 mM	0.5175 mL	2.5873 mL	5.1746 mL	12.9366 mL
	15 mM	0.3450 mL	1.7249 mL	3.4498 mL	8.6244 mL
	20 mM	0.2587 mL	1.2937 mL	2.5873 mL	6.4683 mL
	25 mM	0.2070 mL	1.0349 mL	2.0699 mL	5.1746 mL
	30 mM	0.1725 mL	0.8624 mL	1.7249 mL	4.3122 mL
	40 mM	0.1294 mL	0.6468 mL	1.2937 mL	3.2342 mL
	50 mM	0.1035 mL	0.5175 mL	1.0349 mL	2.5873 mL
	60 mM	0.0862 mL	0.4312 mL	0.8624 mL	2.1561 mL
	80 mM	0.0647 mL	0.3234 mL	0.6468 mL	1.6171 mL
	100 mM	0.0517 mL	0.2587 mL	0.5175 mL	1.2937 mL

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Iminostilbene

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Voir tous les produits spécifiques à Isoform TNF Receptor

Voir tous les produits spécifiques à Isoform HIF/HIF Prolyl-Hydroxylase

Voir tous les produits spécifiques à Isoform STAT

Voir tous les produits spécifiques à Isoform Interleukin Related

Voir tous les produits spécifiques à Isoform COX

Voir tous les produits spécifiques à Isoform NO Synthase

Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

Iminostilbene Related Classifications

HY-N7064 Related Classifications