COX-2-IN-77
COX-2-IN-77 is a selective COX-2 inhibitor with an IC50 of 0.09 μM against ovine COX-2 and lower activity against COX-1 (ovine IC50 of 12.75 μM). COX-2-IN-77 induces Apoptosis and S-phase cell cycle arrest. COX-2-IN-77 upregulates BAX and Caspase-3 expression and downregulates Bcl-2 expression. COX-2-IN-77 exhibits anticancer activity against colorectal adenocarcinoma.
For research use only. We do not sell to patients.
- Formula: C23H18FNO4S
- Molecular Weight:423.46
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
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All Caspase Isoforms
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Biological Activity
Description
IC50 & Target
[1]|
COX-2 0.09 μM (IC50) |
COX-1 12.75 μM (IC50) |
Caspase 3 |
Bax |
Bcl-2 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HT-29 | IC50 |
0.654 μM
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Cytotoxicity against human HT-29 colorectal adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay.
Cytotoxicity against human HT-29 colorectal adenocarcinoma cells assessed as viability reduction incubated 48 hrs by MTT assay.
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42896301 |
| HFF | IC50 |
1000 μM
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Cytotoxicity against human HFF foreskin fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay, with IC50 greater than 1000 μM.
Cytotoxicity against human HFF foreskin fibroblasts assessed as viability reduction incubated 48 hrs by MTT assay, with IC50 greater than 1000 μM.
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42896301 |
| HEK293 | IC50 |
1000 μM
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Cytotoxicity against human HEK293 embryonic kidney cells assessed as viability reduction incubated 48 hrs by MTT assay, with IC50 greater than 1000 μM.
Cytotoxicity against human HEK293 embryonic kidney cells assessed as viability reduction incubated 48 hrs by MTT assay, with IC50 greater than 1000 μM.
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42896301 |
In Vitro
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-29 human colorectal adenocarcinoma cells, HFF human foreskin fibroblasts, and HEK293 human embryonic kidney cells
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Concentration:0.1, 1, 10, and 100 μM in HT-29 cells; 0.1, 1, 10, 100, and 1000 μM in HFF and HEK293 cells
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Incubation Time:48 h
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Result:In HT-29 cells, IC50 = 0.654 μM.
In HFF cells, IC50 >1000 μM.
In HEK293 cells, IC50 >1000 μM.
Selectivity index HFF/HT-29 ≈1529.05; HEK293/HT-29 ≈1529.05.
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Cell Line:HT-29 human colorectal adenocarcinoma cells
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Concentration:0.654 μM (HT-29 IC50)
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Incubation Time:48 h
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Result:G0/G1 was 43.73%, S was 43.20%, and G2/M was 13.62%, indicating increased S-phase and reduced G0/G1 and G2/M populations relative to control.
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Cell Line:HT-29 human colorectal adenocarcinoma cells
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Concentration:0.654 μM (HT-29 IC50)
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Incubation Time:48 h (incubation); 15 min (staining)
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Result:Early apoptosis was 23.45% and late apoptosis was 24.90% in HT-29 cells.
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Cell Line:HT-29 human colorectal adenocarcinoma cells
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Concentration:0.654 μM (HT-29 IC50)
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Incubation Time:48 h
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Result:Significantly upregulated BAX expression, markedly reduced Bcl-2 expression, significantly elevated caspase-3 expression, and significantly decreased VEGF and PTGS2 expression relative to the untreated control.
Chemical Information
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Molecular Weight 423.46
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Formula C23H18FNO4S
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SMILES
O=C(C1=CC=CC=C1)C(C2C3=CC=C(S(=O)(C)=O)C=C3)ON=C2C4=CC=C(F)C=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
COX-2-IN-77 Related Classifications
HY-190701 Related Classifications
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