UBD1031

UBD1031 potently binds purified USP16-UBD (residues 25-185) with an SPR-determined K_D of 48 nM^[1].
UBD1031 potently binds purified HDAC6-UBD (residues 1109-1215) with an SPR-determined K_D of 16 nM^[1].
UBD1031 (0.01 nM-10 μM) inhibits the deubiquitinase activity of purified full-length USP16 (USP16^1-823) with an IC₅₀ of 10.6 μM, reaching up to 60% inhibition at maximal tested concentrations^[1].
UBD1031 is at least 100-fold selective for USP16-UBD and HDAC6-UBD over seven other tested purified UBD-containing proteins (USP3, USP5, USP13, USP33, USP49, USP51, BRAP)^[1].
UBD1031 (0.1-10 μM; 4 h) disrupts the interaction between USP16 and ISG15 in HEK293T cells with an IC₅₀ of 1.7 μM^[1].
UBD1031 (0.1-10 μM; 4 h) disrupts the interaction between HDAC6 and ISG15 in HEK293T cells with an IC₅₀ of 1.5 μM^[1].
UBD1031 (30 μM; 5 day) shows no detectable toxicity to HEK293T or HCT116 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.