Fisetin quarterhydrate
Based on 32 publication(s) in Google Scholar
Fisetin quarterhydrate is a natural flavonol found in many fruits and vegetables with various benefits, such as antioxidant, anticancer, neuroprotection effects.
For research use only. We do not sell to patients.
- Purity: 95.89%
- Formula: C15H10O6.1/4H2O
- Molecular Weight:290.75
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Publications Citing Use of MedChemExpress (MCE) Fisetin quarterhydrate
More- Cell Stem Cell. 2025 Dec 4;32(12):1869-1885.e8. [Abstract]
- Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
- Autophagy. 2025 Jul 27:1-23. [Abstract]
- Cell Rep Med. 2026 Jan 20;7(1):102532. [Abstract]
- Acta Pharmacol Sin. 2023 Oct;44(10):2065-2074. [Abstract]
- Aging Cell. 2026 Jan 20;25(2):e70352.
- JCI Insight. 2025 Jan 23;10(2):e180248. [Abstract]
- Biochem Pharmacol. 2025 Apr 3:116919. [Abstract]
- Mol Cancer Ther. 2025 Jul 2. [Abstract]
- J Ethnopharmacol. 2024 Jun 14:118421. [Abstract]
- Cells. 2022 Jun 21;11(13):1992. [Abstract]
- J Mol Liq. 23 November 2021, 118164.
- Int J Mol Sci. 2026 Jun 22;27(12):5622. [Abstract]
- Int J Mol Sci. 2025 May 22;26(11):4978. [Abstract]
- Biol Direct. 2025 Apr 2;20(1):43. [Abstract]
- J Nutr Biochem. 2023 Dec:122:109452. [Abstract]
- Biomolecules. 2025 Jun 16;15(6):873. [Abstract]
- Eur J Pharmacol. 2024 Feb 5:964:176298. [Abstract]
- Molecules. 2023 Jul 20;28(14):5533. [Abstract]
- Hepatob Pancreat Dis. 2021 Aug;20(4):352-360. [Abstract]
- Eur J Nutr. 2025 Mar 19;64(3):128. [Abstract]
- Biochim Biophys Acta Mol Basis Dis. 2024 Sep 13:167515. [Abstract]
- Fish Shellfish Immunol. 2025 Aug 6:166:110633. [Abstract]
- Aging (Albany NY). 2021 Nov 25;13(22):24753-24767. [Abstract]
- Food Sci Nutr. 2025 Sep 3;13(9):e70892. [Abstract]
- Microbiol Spectr. 2025 Aug 21:e0152725. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2025 Feb;398(2):1665-1679. [Abstract]
- PeerJ. 2025 Mar 28:13:e19198. [Abstract]
- Biochem Biophys Res Commun. 2018 Sep 3;503(1):297-303. [Abstract]
- bioRxiv. 2024 Jul 30:2024.07.29.605645. [Abstract]
- Journal of Harran University Medical Faculty. 2023 Sep 6.
- Research Square Preprint. 2023 Nov 20.
Customer Validation & Images
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Cell Imaging/Staining
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RT-PCR
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Cell Imaging/Staining
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WB
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IF
Biological Activity
Sirtuin, PPAR, TNF-alpha[1][2]
Fisetin quarterhydrate inhibits lipid accumulation and suppresses the expression of PPARγ in 3T3-L1 cells. Fisetin quarterhydrate suppresses early stages of preadipocyte differentiation, and induces expression of Sirt1. Fisetin quarterhydrate facilitates Sirt1-mediated deacetylation of PPARγ and FoxO1, and enhances the association of Sirt1 with the PPARγ promoter, leading to suppression of PPARγ transcriptional activity, thereby repressing adipogenesis[1]. Fisetin quarterhydrate binds to tubulin and stabilizes microtubules with binding characteristics far superior than paclitaxel. Fisetin quarterhydrate treatment of human prostate cancer cells results in robust up-regulation of microtubule associated proteins (MAP)-2 and -4. Fisetin quarterhydrate significantly inhibits PCa cell proliferation, migration, and invasion. Nudc, a protein associated with microtubule motor dynein/dynactin complex that regulates microtubule dynamics, is inhibited with Fisetin quarterhydrate treatment[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 290.75
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Formula C15H10O6.1/4H2O
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Color Light yellow to yellow
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SMILES
O=C1C(O)=C(C2=CC=C(O)C(O)=C2)OC3=CC(O)=CC=C13.O.[1/4]
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Publications (32)
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Journal Impact Factor
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Most Recent
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Cell Stem Cell
Senolytic-sensitive p16Ink4a+ fibroblasts in the tumor stroma rewire lung cancer metabolism and plasticity. [Abstract]2025 Dec 4;32(12):1869-1885.e8. PMID: 41187746 -
Nat Aging
Spatiotemporal transcriptomic changes of human ovarian aging and the regulatory role of FOXP1. [Abstract]2024 Apr;4(4):527-545. PMID: 38594460
Fisetin quarterhydrate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
Fisetin (10 μM, 24 h), Quercetin (10 μM, 24 h) delayed FOXP1 gene silencing-induced cellular senescence in COV434 cells.
Fisetin quarterhydrate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
Fisetin (10 μM, 24 h), Quercetin (10 μM, 24 h) inhibited the FOXP1 silencing-induced increase in CDKN1A expression in COV434 cells.
Fisetin quarterhydrate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
Fisetin (10 μM, 24 h) and Quercetin (10 μM, 24 h) reversed the decrease in COV434 cell proliferation induced by FOXP1 knockdown.
Fisetin quarterhydrate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
Quercetin (10 μM, 24 h) but not Fisetin (10 μM, 24 h) delayed FOXP1 gene silencing in COV434 cells.
Fisetin quarterhydrate purchased from MedChemExpress. Usage Cited in: Nat Aging. 2024 Apr;4(4):527-545. [Abstract]
Quercetin (10 μM, 24 h) but not Fisetin (10 μM, 24 h) decreased γH2AX levels in COV434 cells.
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Autophagy
Restricting intracellular Salmonella proliferation by coordinating p-TBK1 mediated mitophagy and xenophagy. [Abstract]2025 Jul 27:1-23. PMID: 40660474 -
Cell Rep Med
Targeting senescent EGR1+ B cells enhances immunotherapy efficacy in esophageal squamous cell carcinoma. [Abstract]2026 Jan 20;7(1):102532. PMID: 41483805 -
Acta Pharmacol Sin
Fisetin treatment alleviates kidney injury in mice with diabetes-exacerbated atherosclerosis through inhibiting CD36/fibrosis pathway. [Abstract]2023 Oct;44(10):2065-2074. PMID: 37225845 -
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JCI Insight
Neutrophil extracellular traps potentiate effector T cells via endothelial senescence in uveitis. [Abstract]2025 Jan 23;10(2):e180248. PMID: 39846254 -
Biochem Pharmacol
NUF2 activated by YY1 promotes prostate cancer malignancy via p38/MAPK signaling axis and serves as a therapeutic target. [Abstract]2025 Apr 3:116919. PMID: 40187571 -
Mol Cancer Ther
Harnessing senolytics and PARP inhibition to expand the antitumor activity of CDK4/6 inhibitors in prostate cancer. [Abstract]2025 Jul 2. PMID: 40601842 -
J Ethnopharmacol
Study on the anti-skin aging effect and mechanism of Sijunzi Tang based on network pharmacology and experimental validation. [Abstract]2024 Jun 14:118421. PMID: 38880400 -
Cells
EA.hy926 Cells and HUVECs Share Similar Senescence Phenotypes but Respond Differently to the Senolytic Drug ABT-263. [Abstract]2022 Jun 21;11(13):1992. PMID: 35805077 -
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Int J Mol Sci
Synergistic Induction of Caspase-8-Mediated Leukaemic Cell Death by Fisetin and Pinocembrin. [Abstract]2026 Jun 22;27(12):5622. PMID: 42353336 -
Int J Mol Sci
Fisetin Attenuates Zinc Overload-Induced Hepatotoxicity in Mice via Autophagy-Dependent Nrf2 Activation. [Abstract]2025 May 22;26(11):4978. PMID: 40507790 -
Biol Direct
GAMT facilitates tumor progression via inhibiting p53 in clear cell renal cell carcinoma. [Abstract]2025 Apr 2;20(1):43. PMID: 40176130 -
J Nutr Biochem
F6P/G6P-mediated ChREBP activation promotes the insulin resistance-driven hepatic lipid deposition in zebrafish. [Abstract]2023 Dec:122:109452. PMID: 37748621 -
Biomolecules
Targeting Cellular Senescence to Enhance Human Endometrial Stromal Cell Decidualization and Inhibit Their Migration. [Abstract]2025 Jun 16;15(6):873. PMID: 40563513 -
Eur J Pharmacol
Fisetin suppresses ferroptosis through Nrf2 and attenuates intervertebral disc degeneration in rats. [Abstract]2024 Feb 5:964:176298. PMID: 38145645 -
Molecules
Fisetin Delays Postovulatory Oocyte Aging by Regulating Oxidative Stress and Mitochondrial Function through Sirt1 Pathway. [Abstract]2023 Jul 20;28(14):5533. PMID: 37513404 -
Hepatob Pancreat Dis
Fisetin mitigates hepatic ischemia-reperfusion injury by regulating GSK3β/AMPK/NLRP3 inflammasome pathway. [Abstract]2021 Aug;20(4):352-360. PMID: 34024736 -
Eur J Nutr
Fisetin alleviates Salmonella typhimurium-induced colitis through the TLR2/TLR4-NF-κB pathway, regulating microbiota, and repressing intracellular bacterial proliferation by focal adhesion kinase. [Abstract]2025 Mar 19;64(3):128. PMID: 40106033 -
Biochim Biophys Acta Mol Basis Dis
MISP-mediated enhancement of pancreatic cancer growth through the Wnt/β-catenin signaling pathway is suppressed by Fisetin. [Abstract]2024 Sep 13:167515. PMID: 39278512 -
Fish Shellfish Immunol
Emodin enhances host antiviral immunity against Micropterus salmoides rhabdovirus by activating RLR signaling in largemouth bass. [Abstract]2025 Aug 6:166:110633. PMID: 40769268 -
Aging (Albany NY)
Fisetin inhibits the proliferation, migration and invasion of pancreatic cancer by targeting PI3K/AKT/mTOR signaling. [Abstract]2021 Nov 25;13(22):24753-24767. PMID: 34821587 -
Food Sci Nutr
Flavonoids in Rosa roxburghii Tratt Fermentation Broth Ameliorate Obesity via DNMT3a/SIRT1-Mediated Epigenetic Modulation. [Abstract]2025 Sep 3;13(9):e70892. PMID: 40909259 -
Microbiol Spectr
2025 Aug 21:e0152725. PMID: 40838703 -
Naunyn Schmiedebergs Arch Pharmacol
Therapeutic use of fisetin and pirfenidone combination in bleomycin-induced pulmonary fibrosis in adult male albino rats. [Abstract]2025 Feb;398(2):1665-1679. PMID: 39162796 -
PeerJ
Fisetin may protect early porcine embryos from oxidative stress by down-regulating GRP78 levels. [Abstract]2025 Mar 28:13:e19198. PMID: 40166042 -
Biochem Biophys Res Commun
Effects of intracellular iron overload on cell death and identification of potent cell death inhibitors. [Abstract]2018 Sep 3;503(1):297-303. PMID: 29890135 -
bioRxiv
2024 Jul 30:2024.07.29.605645. PMID: 39131266 -
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Solvent & Solubility
DMSO : 100 mg/mL (343.94 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (8.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (8.60 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (277 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Kim SC, et al. Fisetin induces Sirt1 expression while inhibiting early adipogenesis in 3T3-L1 cells. Biochem Biophys Res Commun. 2015 Nov 27;467(4):638-44. [Content Brief]
[2]. Mukhtar E, et al. Dietary flavonoid fisetin binds to β-tubulin and disrupts microtubule dynamics in prostate cancer cells. Cancer Lett. 2015 Oct 28;367(2):173-83. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 3.4394 mL | 17.1969 mL | 34.3938 mL | 85.9845 mL |
| 5 mM | 0.6879 mL | 3.4394 mL | 6.8788 mL | 17.1969 mL | |
| 10 mM | 0.3439 mL | 1.7197 mL | 3.4394 mL | 8.5985 mL | |
| 15 mM | 0.2293 mL | 1.1465 mL | 2.2929 mL | 5.7323 mL | |
| 20 mM | 0.1720 mL | 0.8598 mL | 1.7197 mL | 4.2992 mL | |
| 25 mM | 0.1376 mL | 0.6879 mL | 1.3758 mL | 3.4394 mL | |
| 30 mM | 0.1146 mL | 0.5732 mL | 1.1465 mL | 2.8662 mL | |
| 40 mM | 0.0860 mL | 0.4299 mL | 0.8598 mL | 2.1496 mL | |
| 50 mM | 0.0688 mL | 0.3439 mL | 0.6879 mL | 1.7197 mL | |
| 60 mM | 0.0573 mL | 0.2866 mL | 0.5732 mL | 1.4331 mL | |
| 80 mM | 0.0430 mL | 0.2150 mL | 0.4299 mL | 1.0748 mL | |
| 100 mM | 0.0344 mL | 0.1720 mL | 0.3439 mL | 0.8598 mL |
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