Arnicolide B
Arnicolide B is a sesquiterpene lactone. Arnicolide B is isolated from Centipeda minima. Arnicolide B inhibits the phosphorylation of ERK, JNK and p38 proteins in the MAPK signaling pathway. Arnicolide B reduces the production of inflammatory mediators NO, PGE2 and IL-6. Arnicolide B downregulates the overexpression of inflammatory proteins iNOS and COX-2. Arnicolide B has no effect on IκB-α degradation or NF-κB pathway activation. Arnicolide B is applicable to inflammation-related research.
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- No. CAS: 34532-66-6
- Fòrmula: C20H28O5
- Peso molecular:348.44
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
Descripciòn
IC50 & Target
[1]|
iNOS |
COX-2 |
IL-6 |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HCT-116 | IC50 |
7.2 μM
Compound: 20
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Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human HCT-116 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 33533247] |
| HeLa | IC50 |
5.5 μM
Compound: 20
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Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human HeLa cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 33533247] |
| HepG2 | IC50 |
10 μM
Compound: 20
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Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
Cytotoxicity against human HepG2 cells assessed as reduction in cell viability incubated for 48 hrs by MTT assay
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[PMID: 33533247] |
| RAW264.7 | IC50 |
1.7 μM
Compound: 20
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Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based assay
Anti-inflammatory activity in mouse RAW264.7 cells assessed as inhibition of LPS-induced NO production incubated for 24 hrs by Griess reagent based assay
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[PMID: 33533247] |
In Vitro
Arnicolide B (0.78-100 μM; 24 h) shows no cytotoxicity against RAW 264.7 cells at concentrations ≤6.25 μM[1].
Arnicolide B (0.78-6.25 μM; 24 h) dose-dependently inhibits LPS-induced excessive NO production in RAW 264.7 macrophages[1].
Arnicolide B (0.78-6.25 μM; 24 h) dose-dependently downregulates LPS-induced overexpression of iNOS and COX-2 in RAW 264.7 macrophages[1].
Arnicolide B (0.78-6.25 μM; 10 min) exerts no significant inhibitory effect on LPS-induced IκB-α degradation in RAW 264.7 macrophages, indicating that it has no impact on the NF-κB pathway[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:LPS-activated mouse monocyte-macrophage RAW 264.7 cells
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Concentration:0.78-6.25 μM
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Incubation Time:24 h
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Result:Reduced LPS-induced iNOS expression at 0.78 μM.
Reduced LPS-induced iNOS expression at 1.56 μM.
Reduced LPS-induced iNOS expression at 3.12 μM.
Reduced LPS-induced iNOS expression 6.25 μM.
Reduced LPS-induced COX-2 expression.
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Cell Line:LPS-activated mouse monocyte-macrophage RAW 264.7 cells
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Concentration:0.78-6.25 μM
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Incubation Time:10 min
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Result:Did not alter LPS-induced IκB-α degradation, with IκB-α expression remaining at ~15-20% relative to β-actin, similar to the LPS-only group.
Chemical Information
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No. CAS 34532-66-6
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Peso molecular 348.44
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Fòrmula C20H28O5
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SMILES
O(C(CC(C)C)=O)[C@H]1[C@]2([C@@](C[C@@H](C)[C@]3([C@@]1(C)C(=O)C=C3)[H])(OC(=O)[C@H]2C)[H])[H]
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Arnicolide B Related Classifications
HY-N19215 Related Classifications
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