JAK1/2-IN-3
JAK1/2-IN-3 is a JAK1 and JAK2 inhibitor with IC50 values of 14.73 nM and 10.03 nM, respectively. JAK1/2-IN-3 induces G2/M cell cycle arrest, and induces intrinsic apoptosis via increased Bax and caspase 3/7 levels and reduced Bcl2 expression. JAK1/2-IN-3 can be used for the study of leukemia cancer, breast cancer and colon cancer.
For research use only. We do not sell to patients.
- Formula: C22H13Cl2N5O5
- Molecular Weight:498.28
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All Caspase Isoforms
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Biological Activity
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JAK1 14.73 nM (IC50) |
JAK2 10.03 nM (IC50) |
Caspase 3 |
Caspase-7 |
Bax |
Bcl-2 |
JAK1/2-IN-3 (Compound 3i) inhibits HT-29, MCF-7, HL-60 and HCT-116 cells, with IC50 values of 1.02 μM, 1.87 μM, 7.92 μM and 1.27 μM, respectively. JAK1/2-IN-3 exhibits lower toxicity toward normal WI-38 cells (IC50: 19.93 μM)[1].
JAK1/2-IN-3 (1.02 µM; 24 hours) induces apoptosis in HT-29 cells[1].
JAK1/2-IN-3 (1.02 µM; 24 hours) causes induction of G2/M cell cycle arrest in HT-29 cells[1].
JAK1/2-IN-3 (1.02 µM; 48 hours) increases Bax and caspase 3/7 levels and reduces Bcl2 expression in HT-29 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-29
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Concentration:1.02 µM
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Incubation Time:24 hours
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Result:Significantly increased apoptosis with a 24.39% total apoptosis rate and a 16.9% late apoptotic rate.
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Cell Line:HT-29
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Concentration:1.02 µM
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Incubation Time:24 hours
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Result:Led to a 5.55-fold increase in the G2/M population compared to the untreated HT-29 cells, accompanied by a notable drop in the G0/G1 and S phase populations by around 24.4 % and 38.6 %, respectively.
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Cell Line:HT-29
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Concentration:1.02 µM
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Incubation Time:48 hours
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Result:Showed a 3.34-fold upregulation of Bax, a 0.26-fold downregulation of Bcl2, and 5.96-fold and 9.00-fold increases in caspase-3 and caspase-7, respectively.
Chemical Information
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Molecular Weight 498.28
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Formula C22H13Cl2N5O5
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SMILES
O=C(N(C1=CC=CC=C1)N=C/2NC3=CC=C(Cl)C([N+]([O-])=O)=C3)C2=C\C4=CC=C(Cl)C([N+]([O-])=O)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
JAK1/2-IN-3 Related Classifications
HY-180274 Related Classifications
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