2. Apoptosis Autophagy Metabolic Enzyme/Protease Immunology/Inflammation NF-κB MAPK/ERK Pathway
  3. p62 Atg8/LC3 Apoptosis Autophagy Reactive Oxygen Species JNK Bcl-2 Family Caspase
  4. Astin B

Astin B is a orally active and potent cyclic pentapeptide, that can be isolated from Aster tataricus. Astin B has hepatotoxic effects in vitro and in vivo and that hepatic injury was primarily mediated by apoptosis in a mitochondria/caspase-dependent manner. Astin B induces autophagy in L-02 cells, increases LC3-II and decreases p62 expression.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Astin B Chemical Structure

Astin B Chemical Structure

CAS No. : 151201-76-2

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Astin B Related Antibodies

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  • Biological Activity

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Description

Astin B is a orally active and potent cyclic pentapeptide, that can be isolated from Aster tataricus. Astin B has hepatotoxic effects in vitro and in vivo and that hepatic injury was primarily mediated by apoptosis in a mitochondria/caspase-dependent manner. Astin B induces autophagy in L-02 cells, increases LC3-II and decreases p62 expression[1].

IC50 & Target

Bcl-2

 

Bax

 

Caspase-3

 

Caspase-9

 

In Vitro

Astin B (0-60 μM, 12-48 h) inhabits proliferation of L-02 cells[1].
Astin B (0-60 μM, 24 h) induces oxidative stress and mitochondrial dysfunction, regulates Bcl-2/Bax expression and increases caspase-3 and -9 activity[1].
Astin B (0-60 μM, 24 h) induces autophagy in L-02 cells to protect from apoptosis[1].
Astin B provokes oxidative stress-associated inflammation in hepatocytes as evidenced by increased levels of reactive oxygen species (ROS), reduced contents of intracellular glutathione (GSH), and enhanced phosphorylation of c-Jun N-terminal kinase (JNK)[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Astin B Related Antibodies
In Vivo

Astin B (10 mg/kg, Orally, once daily for 7 consecutive days) induces liver injury in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male ICR mice (6–8 weeks of age, 10 per group)[1]
Dosage: 10 mg/kg
Administration: Orally administered, once daily for 7 consecutive days
Result: Induced liver injury in mice. Compared to the control group, the serum levels of ALT and AST in mice treated with astin B were elevated about 11.9 and 2.26 times, respectively.
Molecular Weight

586.46

Formula

C25H33Cl2N5O7
CAS No.
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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Astin B Related Classifications

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Astin B151201-76-2p62Atg8/LC3ApoptosisAutophagyReactive Oxygen SpeciesJNKBcl-2 FamilyCaspaseSequestosome-1SQSTM1Autophagy-related 8c-Jun N-terminal kinaseLiver injuryAster tataricusL-02 cellslactate dehydrogenaseLDHInhibitorinhibitorinhibit

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