Cat. No.: HY-N0836

Jervine

(Synonyms: 11-Ketocyclopamine)

3 Cited Publications

Customer Review

Based on 3 publication(s) in Google Scholar

Jervine (11-Ketocyclopamine) is a potent Hedgehog (Hh) inhibitor with an IC₅₀ of 500-700 nM. Jervine is a natural teratogenic sterodial alkaloid from rhizomes of Veratrum nigrum. Jervine has anti-inflammatory and antioxidant properties.

For research use only. We do not sell to patients.

Purity: 99.28%
CAS No.: 469-59-0
Formula: C₂₇H₃₉NO₃
Molecular Weight:425.60

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Jervine Chemical Structure

CAS No.: 469-59-0

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Publications (3)
Solvent & Solubility
- In Vitro
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

Jervine (11-Ketocyclopamine) is a potent Hedgehog (Hh) inhibitor with an IC₅₀ of 500-700 nM^[1]. Jervine is a natural teratogenic sterodial alkaloid from rhizomes of Veratrum nigrum. Jervine has anti-inflammatory and antioxidant properties^[2].

IC₅₀ & Target

IC50: 500-700 nM (Hedgehog)^[1]

In Vitro

Jervine (40 μM; 6, 12 and 24 hours) inhibits Akt phosphorylation^[3].
Jervine (40 μM; 2 hours) inhibits NF-jB activation decreased COX-2 overexpression and induced apoptosis^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

HEL and TF1a cells
Concentration:

40 μM
Incubation Time:

6, 12 and 24 hours
Result:

Inhibited Akt phosphorylation.

In Vivo

Jervine (orally; 50-400 mg/kg) exerts 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male Sprague Dawley rats (180-200  g)^[2]
Dosage:

50, 100, 200 and 400  mg/kg
Administration:

Orally
Result:

Exerted 50.4-73.5% anti-inflammatory effects in carrageenan induced paw edema.

Chemical Information

CAS No. 469-59-0
Appearance Solid
Molecular Weight 425.60
Formula C₂₇H₃₉NO₃
Color White to off-white
SMILES

O=C1[C@]2([H])[C@]3(C)C(C[C@@H](O)CC3)=CC[C@@]2([H])[C@]4([H])CC[C@]5(O[C@@]6([H])[C@@]([C@H]5C)([H])NC[C@@H](C)C6)C(C)=C41
Synonyms

11-Ketocyclopamine
Structure Classification
- Alkaloids
- Piperidine Alkaloids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (3)

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Journal Impact Factor

Philos Trans R Soc Lond B Biol Sci

Small molecule activates citrullination through targeting PAD2. [Abstract]

2023 Nov 20;378(1890):20220248. PMID: 37778388
J Radiat Res Appl Sci

2025 Sep.
Biochem Biophys Res Commun

Jervine inhibits non-small cell lung cancer (NSCLC) progression by suppressing Hedgehog and AKT signaling via triggering autophagy-regulated apoptosis. [Abstract]

2020 Dec 10;533(3):397-403. PMID: 32972750

Solvent & Solubility

In Vitro:

DMSO : 1 mg/mL (2.35 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.3496 mL

11.7481 mL

23.4962 mL

5 mM

---

10 mM

---

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (644 KB)
- English - EN (644 KB)
- Français - FR (644 KB)
- Deutsch - DE (644 KB)
- Norwegian - NO (644 KB)
- Español - ES (644 KB)
- Swedish - SV (644 KB)
- Italian - IT (644 KB)
- Korean - KR (644 KB)
- Portuguese - PT (644 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Williams JA, et al.Identification of a small molecule inhibitor of the hedgehog signaling pathway: effects on basalcell carcinoma-like lesions.Proc Natl Acad Sci U S A. 2003 Apr 15;100(8):4616-21. [Content Brief]

[2]. Dumlu FA, et al.Anti-inflammatory and antioxidant properties of jervine, a sterodial alkaloid from rhizomes of Veratrum album.Phytomedicine. 2019 Mar 1;55:191-199. [Content Brief]

[3]. Ghezali L, et al.Cyclopamine and jervine induce COX-2 overexpression in human erythroleukemia cells but only cyclopamine has a pro-apoptotic effect.Exp Cell Res. 2013 Apr 15;319(7):1043-53. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.3496 mL	11.7481 mL	23.4962 mL	58.7406 mL

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Jervine

Immunology/Inflammation Compound Library

Stem Cell Signaling Compound Library

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Publications Citing Use of MedChemExpress (MCE) Jervine