Cat. No.: HY-N2014

Verbenalin

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Verbenalin is an orally active terpenoid glycoside that can be extracted from the medicinal plant Verbena officinalis. Verbenalin has anti-inflammatory, antiviral, and neuroprotective effects. Verbenalin has a strong binding affinity to the nsp-12 protein of the SARS-CoV-2 virus. Verbenalin can be used in the research of inflammatory and nervous system diseases such as hepatitis and Alzheimer's disease.

For research use only. We do not sell to patients.

Purity: 99.93%
CAS No.: 548-37-8
Formula: C₁₇H₂₄O₁₀
Molecular Weight:388.37

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Verbenalin Chemical Structure

CAS No.: 548-37-8

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

All Caspase Isoforms :

All Bcl-2 Family Isoforms :

In Vitro

Verbenalin (1-100 μM; 24 h) can downregulate the levels of APP and Aβ42 in APP-overexpressing N2a cells, while there is no significant change in the level of Aβ40^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[1]

Cell Line:

APP-overexpressing N2a cells
Concentration:

1, 3, 10, 30 and 100 μM
Incubation Time:

24 h
Result:

Inhibited the APP protein levels in cell lysate and supernatant.

In Vivo

Verbenalin (100-200 mg/kg; gavage; 35 days) reduces the levels of Aβ and tau in the hippocampus and restores the expression of BDNF in the AD mouse model^[1].
Verbenalin (50-200 mg/kg; gavage; 21 days) has an improving effect in the mouse prostatitis model^[2].
Verbenalin (5-20 mg/kg; intravenous injection; single dose) has a protective effect in the rat model of focal cerebral ischemia-reperfusion^[3].
Verbenalin (12.5-50 mg/kg; gavage; 16 days) can alleviate liver injury, regulate lipid homeostasis, inhibit oxidative stress-induced ferroptosis, and improve mitochondrial function in the mouse model of alcoholic steatohepatitis^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

KM male mice (24-26 g) treated Xiaozhiling to induce prostatitis model^[2]
Dosage:

50, 100 and 200 mg/kg
Administration:

Oral gavage (i.g.); 21 days
Result:

Had significantly increased amounts of water, and prostate white blood cell count and prostate volume density were decreased significantly, the density of lecithin corpuscle score increased, and pathologic prostatitis changes were significantly reduced.
Significantly reduced pathological change in the testis and the change in the epididymis.
Increased significantly the thymic cortex thickness and the number of lymphocytes.
Reduce the renal pathological changes in potential.

Animal Model:

Male KM rats (25-30 g) with focal cerebral ischemia reperfusion^[3]
Dosage:

5, 10 and 20 mg/kg
Administration:

Intravenous injection (i.v.); single dose
Result:

Significantly reduced the mortality rate, neurological deficit score and the percentage of cerebral infarction size.
Significantly reduced the levels of Bax, Caspase-3, S-100β level of the serum in the brain tissue.
Increased the levels of Bcl-2 and ATPase in brain tissue and improved pathological damage of hippocampus and cortex.
Inhibited the expression of apoptosis genes, promoted the expression of anti-apoptosis genes, improved brain microcirculation and energy metabolism, hence reducing cerebral ischemia-reperfusion injury.

Chemical Information

CAS No. 548-37-8
Appearance Solid
Molecular Weight 388.37
Formula C₁₇H₂₄O₁₀
Color White to off-white
SMILES

O=C(C1=CO[C@@H](O[C@H]2[C@@H]([C@H]([C@@H]([C@@H](CO)O2)O)O)O)[C@@]3([H])[C@]1([H])C(C[C@@H]3C)=O)OC
Structure Classification
- Terpenoids
- Iridoids
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (2)

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Journal Impact Factor

Mol Biol Rep

Protective effects of verbenalin and (+)-eudesmin against 6-hydroxydopamine-induced oxidative/nitrosative stress in SH-SY5Y cells. [Abstract]

2023 Jan;50(1):331-338. PMID: 36331750
Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (257.49 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5749 mL

12.8743 mL

25.7486 mL

5 mM

0.5150 mL

2.5749 mL

5.1497 mL

10 mM

0.2575 mL

1.2874 mL

2.5749 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.44 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

Working Solution

For the following dissolution methods, please prepare the working solution directly:

It is recommended to prepare fresh solutions and use them promptly within a short period of time.

The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 50% PEG300 50% Saline

Solubility: 25 mg/mL (64.37 mM); Clear solution; Need ultrasonic

Protocol 2

Add each solvent one by one: 0.5% CMC-Na/saline water

Solubility: 50 mg/mL (128.74 mM); Clear solution; Need ultrasonic

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (282 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Lim J, et al. Verbenalin Reduces Amyloid-Beta Peptide Generation in Cellular and Animal Models of Alzheimer's Disease. Molecules. 2022 Dec 8;27(24):8678. [Content Brief]

[2]. Miao M, et al. Effects of verbenalin on prostatitis mouse model. Saudi J Biol Sci. 2016 Jan;23(1):S148-57. [Content Brief]

[3]. Cao L, et al. The protective role of verbenalin in rat model of focal cerebral ischemia reperfusion. Saudi J Biol Sci. 2018 Sep;25(6):1170-1177. [Content Brief]

[4]. Hafiza Salaha Mahrosh, et al. An in silico approach to target RNA-dependent RNA polymerase of COVID-19 with naturally occurring phytochemicals. Environ Dev Sustain. 2021 Apr 3;1-14. [Content Brief]

[5]. Dong J, et al. Verbenalin attenuates hepatic damage and mitochondrial dysfunction in alcohol-associated steatohepatitis by regulating MDMX/PPARα-mediated ferroptosis. J Ethnopharmacol. 2023 May 10;307:116227. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5749 mL	12.8743 mL	25.7486 mL	64.3716 mL
	5 mM	0.5150 mL	2.5749 mL	5.1497 mL	12.8743 mL
	10 mM	0.2575 mL	1.2874 mL	2.5749 mL	6.4372 mL
	15 mM	0.1717 mL	0.8583 mL	1.7166 mL	4.2914 mL
	20 mM	0.1287 mL	0.6437 mL	1.2874 mL	3.2186 mL
	25 mM	0.1030 mL	0.5150 mL	1.0299 mL	2.5749 mL
	30 mM	0.0858 mL	0.4291 mL	0.8583 mL	2.1457 mL
	40 mM	0.0644 mL	0.3219 mL	0.6437 mL	1.6093 mL
	50 mM	0.0515 mL	0.2575 mL	0.5150 mL	1.2874 mL
	60 mM	0.0429 mL	0.2146 mL	0.4291 mL	1.0729 mL
	80 mM	0.0322 mL	0.1609 mL	0.3219 mL	0.8046 mL
	100 mM	0.0257 mL	0.1287 mL	0.2575 mL	0.6437 mL

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Verbenalin

Anti-Infection Compound Library

Apoptosis Compound Library

Immunology/Inflammation Compound Library

Neuronal Signaling Compound Library

Natural Product Library

Antiviral Compound Library

Anti-Aging Compound Library

Glycoside Compound Library

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Anti-Cardiovascular Disease Compound Library

Ferroptosis Compound Library

Medicine Food Homology Compound Library

Terpenoids Library

Orally Active Compound Library

Traditional Chinese Medicine Active Compound Library

Anti-Alzheimer's Disease Compound Library

Neuroprotective Compound Library

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Tonifying Traditional Chinese Medicine Monomer Compound Library

Mass Spectrometry Natural Product Library

Publications Citing Use of MedChemExpress (MCE) Verbenalin

All Caspase Isoforms

All Bcl-2 Family Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Verbenalin Related Classifications

HY-N2014 Related Classifications