Bruceoside B
Bruceoside B is a PI3K/Akt/NF-κB inhibitor. Bruceoside B reduces LPS (HY-D1056)-induced nitric oxide release and the secretion of TNF-α, IL-6 and IL-1β, and decreases the activation level of apoptosis. Bruceoside B inhibits the replication, coat protein accumulation and local lesion formation of tobacco mosaic virus (TMV), and enhances the anti-infection ability of plants. Bruceoside B can be used in studies related to acute lung injury and tobacco mosaic virus infection.
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- No. CAS: 69687-69-0
- Fòrmula: C32H42O16
- Peso molecular:682.67
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Actividad biológica
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Chemical Information
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Actividad biológica
Bruceoside B (24 h) inhibits LPS-induced NO release in MH-S cells, with an IC50 of 45.56 μM[1].
Bruceoside B (10-40 μM; 24 h) dose-dependently inhibits LPS-induced secretion of TNF-α, IL-6, and IL-1β in MH-S cells[1].
Bruceoside B (10-40 μM) regulates the expression of NF-κB pathway and apoptosis-related proteins in LPS-stimulated MH-S cells, reduces the ratios of p-IκB-α/IκB-α and Bax/Bcl-2, downregulates p-NF-κB (with no change in NF-κB expression), and decreases the expression level of full-length Caspase-3 relative to β-actin[1].
Bruceoside B (20 μM; 48 h) inhibits the accumulation of TMV coat protein in leaf discs of Nicotiana tabacum cv. K326, indicating that it suppresses viral replication[2].
Bruceoside B (1.25-20 μM; 3-4 days) inhibits the formation of TMV local lesions in Nicotiana glutinosa leaf tissues, with an IC50 of 4.64 μM, and exhibits dose-dependent anti-TMV activity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:murine alveolar macrophage MH-S cells
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Concentration:10, 20 and 40 μM
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Incubation Time:24 h
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Result:Significantly suppressed LPS-induced secretion of TNF-α, IL-6, and IL-1β in a dose-dependent manner, with statistically significant inhibition observed at all tested concentrations compared to the LPS-only group.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:BALB/c (male, 6-8 weeks old, average weight 20 g)[1]
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Dosage:1 mg/kg; 2 mg/kg; 4 mg/kg
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Administration:i.p.; single dose
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Result:Significantly improved LPS-induced lung tissue damage at all tested doses, with the 4 mg/kg dose showing the most pronounced effect: alveolar collapse, consolidation, compression, and inflammatory cell infiltration were significantly reduced compared to the LPS-only model group.
Dose-dependently blocked LPS-induced phosphorylation of PI3K and Akt in lung tissue, with significant reductions in p-PI3K/PI3K and p-Akt/Akt ratios observed at all doses.
Detected trace levels of brusatol in mouse plasma, indicating bruceoside B is catabolized to brusatol in vivo.
Chemical Information
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No. CAS 69687-69-0
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Peso molecular 682.67
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Fòrmula C32H42O16
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SMILES
C(OC)(=O)[C@]12[C@]3([C@]4([C@@]([C@]5(C)[C@@](C[C@]4(OC(=O)[C@@H]3OC(C=C(C)C)=O)[H])(C(C)=C(O[C@@H]6O[C@H](CO)[C@@H](O)[C@H](O)[C@H]6O)C(=O)C5)[H])([C@@H](O)[C@@H]1O)[H])CO2)[H]
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Pureza y Documentación
Referencias
[1]. He X, et al. Quassinoids from Brucea javanica and attenuates lipopolysaccharide-induced acute lung injury by inhibiting PI3K/Akt/NF-κB pathways. Fitoterapia. 2021 Sep;153:104980. [Content Brief]
[2]. Yan XH, et al. Anti-tobacco mosaic virus (TMV) Quassinoids from Brucea javanica (L.) Merr. Journal of agricultural and food chemistry. 2010 Feb 10;58(3):1572-7. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bruceoside B Related Classifications
HY-N20733 Related Classifications
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