HDAC-IN-43
HDAC-IN-43 is a potent HDAC 1/3/6 inhibitor with IC50 values of 82, 45, and 24 nM, respectively. HDAC-IN-43 is a weak PI3K/mTOR inhibitors with IC50 values of 3.6 and 3.7 μM, respectively. HDAC-IN-43 shows broad anti-proliferative activity .
For research use only. We do not sell to patients.
- CAS No.: 1809163-24-3
- Formula: C22H28N6O4
- Molecular Weight:440.50
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
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HDAC6 24 nM (IC50) |
HDAC3 45 nM (IC50) |
HDAC1 82 nM (IC50) |
HDAC10 182 nM (IC50) |
HDAC2 358 nM (IC50) |
HDAC8 1441 nM (IC50) |
HDAC11 9061 nM (IC50) |
In Vitro
HDAC-IN-43 demonstrats broad anti-cancer activities against 38 cancer cell lines at 72 hours [1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:K562, MOLT4, MV4-11, Raji, Ramos, SU-DHL-6, HepG2, HuH-7, PLC/PRF/5, SK-HEP1, SNU-387, SNU-398, MCF7 and PC-2[1]
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Concentration:0.22-1.42 μM
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Incubation Time:72 h
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Result:showed anti-cancer activities, the IC50 values of K562, MOLT4, MV4-11, Raji, Ramos, SU-DHL-6, HepG2, HuH-7, PLC/PRF/5, SK-HEP1, SNU-387, SNU-398, MCF7 and PC-2 are 0.22-1.42 μM[1].
Chemical Information
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CAS No. 1809163-24-3
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Molecular Weight 440.50
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Formula C22H28N6O4
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SMILES
O=C(NO)CCCOC1=CC=CC(C2=C3N=CN(C(C)C)C3=NC(N4CCOCC4)=N2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HDAC-IN-43 Related Classifications
HY-147966 Related Classifications
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