製品番号: HY-N1365

Isoscopoletin

(別名: 6-Hydroxy-7-methoxycoumarin; イソスコポレチン)

1 Cited Publications

カスタマーレビュー

Based on 1 publication(s) in Google Scholar

Isoscopoletin (6-Hydroxy-7-methoxycoumarin) is an active constituent in Artemisia argyi leaves. Isoscopoletin shows substantial inhibition against cell proliferation, with IC₅₀s of 4.0 μM and 1.6 μM for human CCRF-CEM leukaemia cells and multidrug resistant subline CEM/ADR5000, respectively. Isoscopoletin (6-Hydroxy-7-methoxycoumarin) possesses inhibitory activity against HBV replication. Isoscopoletin exhibits anti-inflammatory activity through inhibition of MAPK/NF-κB/STAT/AKT signaling pathway.

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純度: 99.92%
CAS 番号: 776-86-3
分子式: C₁₀H₈O₄
分子量:192.17

保管条件:

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Isoscopoletin 構造式

CAS 番号: 776-86-3

容量

価格（税別）

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数量

Amount: USD 0.00

* アイテムを追加する前、数量をご選択ください

Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

生物活性

製品説明

Isoscopoletin (6-Hydroxy-7-methoxycoumarin) is an active constituent in Artemisia argyi leaves. Isoscopoletin shows substantial inhibition against cell proliferation, with IC₅₀s of 4.0 μM and 1.6 μM for human CCRF-CEM leukaemia cells and multidrug resistant subline CEM/ADR5000, respectively^[1]. Isoscopoletin (6-Hydroxy-7-methoxycoumarin) possesses inhibitory activity against HBV replication^[2]. Isoscopoletin exhibits anti-inflammatory activity through inhibition of MAPK/NF-κB/STAT/AKT signaling pathway^[3].

p38 MAPK アイソフォーム固有の製品をすべて表示 :

NF-κB アイソフォーム固有の製品をすべて表示 :

STAT アイソフォーム固有の製品をすべて表示 :

Akt アイソフォーム固有の製品をすべて表示 :

ERK アイソフォーム固有の製品をすべて表示 :

JNK アイソフォーム固有の製品をすべて表示 :

Cellular Effect

Cell Line	Type	Value	Description	References
Neutrophil	IC₅₀	77.52 μM Compound: 13	Antiinflammatory activity in human neutrophils assessed as fMLP-induced superoxide release after 5 mins by spectrometry Antiinflammatory activity in human neutrophils assessed as fMLP-induced superoxide release after 5 mins by spectrometry	[PMID: 17822293]
Sf21	IC₅₀	>100 μM Compound: 8	Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay Inhibition of human recombinant COX2 expressed in insect Sf21 cells by EIA assay	[PMID: 17378609]

体外実験

Isoscopoletin (4-100 μg/mL, 12 days) inhibits the secretion of HBsAg (hepatitis B virus surface antigen) and HBeAg (hepatitis B virus e antigen), reduces HBV DNA levels, exhibits inhibitory activity against HBV replication^[2].
Isoscopoletin (0-40 μM, 24 h) inhibits intracellular glucose consumption and lactate production, blocks glycolysis and the energy supply of tumor cells, leading to the inhibition of HepG2 proliferation^[3].
Isoscopoletin (0-40 μM, 1 h) inhibits the inhibited the secretion of TARC, MDC, MCP-1, IL-8 and IL-1β induced by TNF-α/IFN-γ in HaCaT cell, inhibits the secretion of IL-4 induced by PMA (HY-18739)/Ionomycin (HY-13434) in RBL-2H3 cells^[4].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[3]

Cell Line:

HepG2
Concentration:

0-40 μM
Incubation Time:

24 h
Result:

Inhibited the proliferation.

Real Time qPCR^[4]

Cell Line:

HaCaT
Concentration:

0-40 μM
Incubation Time:

1 h
Result:

Downregulated the levels of TARC, MDC, MCP-1, IL-8 and IL-1β.

化学情報

CAS 番号 776-86-3
性状 Solid
分子量 192.17
分子式 C₁₀H₈O₄
Color Off-white to light brown
SMILES

O=C1C=CC2=CC(O)=C(OC)C=C2O1
別名

6-Hydroxy-7-methoxycoumarin; イソスコポレチン
Structure Classification
- Phenylpropanoids
- Coumarins
- Phenols
- Monophenols
Initial Source
- Plants
- Compositae
- Plants
- other families
輸送条件

Room temperature in continental US; may vary elsewhere.
保管条件

4°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (1)

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Vet Microbiol

The Chinese medicine monomer Schisandrin C inhibits PRRSV infection by regulating the OGT-PI3K/AKT/mTOR signaling pathway. [Abstract]

2026 May:316:110992. PMID: 41865607

溶剤 & 溶解度

体外:

DMSO : 100 mg/mL (520.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

5.2037 mL

26.0186 mL

52.0373 mL

5 mM

1.0407 mL

5.2037 mL

10.4075 mL

10 mM

0.5204 mL

2.6019 mL

5.2037 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

モル濃度計算機

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

濃度濃度

体積体積

分子量分子量

希釈計算機

濃度 _(開始) × 体積 _(開始) = 濃度 _(終了) × 体積 _(終了)

濃度 _(開始) 濃度 _(開始)

体積 _(開始) 体積 _(開始)

濃度 _(終了) 濃度 _(終了)

体積 _(終了) 体積 _(終了)

体内:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (13.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (13.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (13.01 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

純度とドキュメンテーション

バッチを選択:

純度: 99.98%

Assay: 99.98%

ee.: 99.98%

データシート (276 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
元素分析報告書 (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
取扱説明書 (2659 KB)

参考文献

[1]. Adams M, et al. Activity-guided isolation of scopoletin and isoscopoletin, the inhibitory active principles towards CCRF-CEM leukaemia cells and multi-drug resistant CEM/ADR5000 cells, from Artemisia argyi. Planta Med. 2006 Jul;72(9):862-4. [Content Brief]

[2]. Li H, et al. Evaluation of antiviral activity of compounds isolated from Ranunculus sieboldii and Ranunculus sceleratus. Planta Med. 2005 Dec;71(12):1128-33. [Content Brief]

[3]. Ruan C, et al., Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins. PLoS One. 2024 Nov 7;19(11):e0310530. [Content Brief]

[4]. Seo DY, et al., Effect of Isoscopoletin on Cytokine Expression in HaCaT Keratinocytes and RBL-2H3 Basophils: Preliminary Study. Int J Mol Sci. 2024 Jun 24;25(13):6908. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	5.2037 mL	26.0186 mL	52.0373 mL	130.0931 mL
	5 mM	1.0407 mL	5.2037 mL	10.4075 mL	26.0186 mL
	10 mM	0.5204 mL	2.6019 mL	5.2037 mL	13.0093 mL
	15 mM	0.3469 mL	1.7346 mL	3.4692 mL	8.6729 mL
	20 mM	0.2602 mL	1.3009 mL	2.6019 mL	6.5047 mL
	25 mM	0.2081 mL	1.0407 mL	2.0815 mL	5.2037 mL
	30 mM	0.1735 mL	0.8673 mL	1.7346 mL	4.3364 mL
	40 mM	0.1301 mL	0.6505 mL	1.3009 mL	3.2523 mL
	50 mM	0.1041 mL	0.5204 mL	1.0407 mL	2.6019 mL
	60 mM	0.0867 mL	0.4336 mL	0.8673 mL	2.1682 mL
	80 mM	0.0650 mL	0.3252 mL	0.6505 mL	1.6262 mL
	100 mM	0.0520 mL	0.2602 mL	0.5204 mL	1.3009 mL

Keywords

Isoscopoletin

1 Cited Publications

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Isoscopoletin

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MedChemExpress（MCE）の使用を引用している文献 Isoscopoletin

p38 MAPK アイソフォーム固有の製品をすべて表示

NF-κB アイソフォーム固有の製品をすべて表示

STAT アイソフォーム固有の製品をすべて表示