Cat. No.: HY-179409

MC3817

Name: MC3817
Brand: MedChemExpress
SKU: HY-179409
Price: 2560.00 USD
Availability: InStock
Rating: 5 (1 reviews)

고객리뷰

Based on 1 Customer Validation

MC3817 is a selective DNMT1 inhibitor. MC3817 inhibits DNMT1 and DNMT3A/3L with IC₅₀s of 0.044 μM and > 10μM, respectively. MC3817 inhibits P53-dependent cancer cell proliferation, induces apoptosis and DNA damage MC3817 elevates cleaved Caspase 3, P53, and γH2AX. MC3817 can be used in non-small cell lung cancer, colon cancer, cervical cancer, triple-negative breast cancer and histiocytic lymphoma research.

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

Purity: 99.88%
화학식: C₃₃H₄₁Cl₄N₉O
분자량:721.55

보관:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

MC3817 Chemical Structure

사이즈

가격

재고

수량

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- 제품 설명
- IC₅₀ & Target
- In Vitro
Chemical Information
용액&용해도
- In Vitro
- In Vivo
순도&문서
References
Help & FAQs
Classifications
Bioz

Biological Activity

제품 설명

IC₅₀ & Target^[1]

DNMT1

0.044 μM (IC₅₀)

All DNA Methyltransferase Isoforms :

All Caspase Isoforms :

All DNA/RNA Synthesis Isoforms :

In Vitro

MC3817 (compound 14) (0.05-100 μM, 48 h) exhibits antiproliferative activity against A549 and H460 (non-small cell and lung cancer), HCT-116 (colon cancer), HeLa (cervical cancer), MDA-MB-231 (triple-negative breast cancer), and U937 (histiocytic lymphoma) cells^[1].
MC3817 (2 μM, 24 and 48 h) induces S/G2M phase arrest and triggers apoptotic cell death in HCT-116 cells^[1].
MC3817 (0.5-5 μM, 16-48 h) triggers DNA damage and activates the p53/γH2AX pathway, leading to cell-cycle arrest and apoptosis in HCT 116 cells^[1].
MC3817 (2.5 μM, 48 h) induces a much higher level of apoptosis in HCT-116 P53 WT cells compared with HCT-116 P53−/− cells ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay^[1]

Cell Line:

A549 , H460, HCT-116, HeLa , MDA-MB 231, and U937
Concentration:

0.05-100 μM
Incubation Time:

48 h
Result:

Had IC₅₀ of 5.5 μM, 6.8 μM, 0.4 μM, 4.2 μM, 1.9 μM and 5.5μM for A549 cells, H460 cells, HCT-116 cells, HeLa cells, MDA-MB 231 cells, and U937 cells.

Cell Cycle Analysis^[1]

Cell Line:

HCT-116 cells
Concentration:

2 μM
Incubation Time:

48 h
Result:

Caused a block in the S/G2M phase associated with apoptotic cell death, affecting over 50% of the cells after 48 h.

Western Blot Analysis^[1]

Cell Line:

HCT-116 cells
Concentration:

0.5, 1, 2.5, 5 μM
Incubation Time:

48 h
Result:

Induced the accumulation of γH2AX and elevated p53 levels, indicating p53 stabilization and activation in response to DNA damage.

Apoptosis Analysis^[1]

Cell Line:

HCT-116 P53^−/− cells and HCT-116 P53 WT cells.
Concentration:

2.5 μM
Incubation Time:

48 h
Result:

Induced apoptotic cell death, as evidenced by significant PI/Annexin V fluorescence and extensive membrane blebbing in both cell lines.

Chemical Information

Appearance Solid
분자량 721.55
화학식 C₃₃H₄₁Cl₄N₉O
Color Light yellow to yellow
SMILES

O=C(NC1=CC(CN(C)C)=CC(NC2=NC(N)=NC(C)=C2)=C1)C3=CC(CN(C)C)=CC(NC4=C5C=CC=CC5=NC=C4)=C3.Cl.Cl.Cl.Cl
선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 100 mg/mL (138.59 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.3859 mL

6.9295 mL

13.8591 mL

5 mM

0.2772 mL

1.3859 mL

2.7718 mL

10 mM

0.1386 mL

0.6930 mL

1.3859 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

농도 희석 계산기

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (3.46 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

순도&문서

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (277 KB)
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Zwergel C, et al. Quinoline-Based DNA Methyltransferase Inhibitors Featuring Basic Side Chains: Design, Synthesis, and Insight in Biochemical and Anticancer Cell Properties. J Med Chem. 2025 Nov 27;68(22):23886-23909. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.3859 mL	6.9295 mL	13.8591 mL	34.6476 mL
	5 mM	0.2772 mL	1.3859 mL	2.7718 mL	6.9295 mL
	10 mM	0.1386 mL	0.6930 mL	1.3859 mL	3.4648 mL
	15 mM	0.0924 mL	0.4620 mL	0.9239 mL	2.3098 mL
	20 mM	0.0693 mL	0.3465 mL	0.6930 mL	1.7324 mL
	25 mM	0.0554 mL	0.2772 mL	0.5544 mL	1.3859 mL
	30 mM	0.0462 mL	0.2310 mL	0.4620 mL	1.1549 mL
	40 mM	0.0346 mL	0.1732 mL	0.3465 mL	0.8662 mL
	50 mM	0.0277 mL	0.1386 mL	0.2772 mL	0.6930 mL
	60 mM	0.0231 mL	0.1155 mL	0.2310 mL	0.5775 mL
	80 mM	0.0173 mL	0.0866 mL	0.1732 mL	0.4331 mL
	100 mM	0.0139 mL	0.0693 mL	0.1386 mL	0.3465 mL

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MC3817

All DNA Methyltransferase Isoforms

All Caspase Isoforms

All DNA/RNA Synthesis Isoforms

Biological Activity

Chemical Information

용액&용해도

순도&문서

References

Help & FAQs

Complete Stock Solution Preparation Table

MC3817 Related Classifications

HY-179409 Related Classifications

Bioz