Cat. No.: HY-W002116

Methyl syringate

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Methyl syringate is a selective TRPA1 agonist. Methyl syringate regulates food intake and gastric emptying through a TRPA1-mediated pathway. Methyl syringate is an efficient phenolic mediator for bacterial and fungal laccases. Methyl syringate is a chemical marker of Asphodel monofloral honey. Methyl syringate contributes to the antibacterial activity of honey. Methyl syringate inhibits aflatoxin production. Methyl syringate can contribute to weight suppression. Methyl syringate can be studies for cancer prevention (e.g. lung cancer), suppression of hypoxia-induced inflammatory response and tumorigenesis.

For research use only. We do not sell to patients.

Purity: 99.96%
CAS No.: 884-35-5
Formula: C₁₀H₁₂O₅
Molecular Weight:212.20

Storage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Methyl syringate Chemical Structure

CAS No.: 884-35-5

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Top Publications Citing

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
- In Vivo
Chemical Information
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Bioz

Biological Activity

Description

IC₅₀ & Target

TRPA1^[3].

All TRP Channel Isoforms :

All COX Isoforms :

In Vitro

Methyl syringate (0.1-1 mM, pretreatment for 1 h then co-treatment under hypoxia for 24 h) effectively blocks hypoxia-induced COX-2 protein expression is a dose dependent manner and inhibits induction of COX-2 mRNA and promoter activity in A549 cells under hypoxic conditions^[4].
Methyl syringate (1 mM, 1 h) represses COX-2 and cell migration in HT-1080 cells^[4].
Methyl syringate (1-100 μM) inhibits catalytic activity of PTPN2 and PTPN6 with IC₅₀ values of 6.95 and 7.31 μM in E. coli cells overexpressing PTPN2 and PTPN6^[5].
Methyl syringate (10-20 μM, 6 h) stimulates the glucose uptake in differentiated 3T3-L1 adipocytes and C2C12 muscle cells^[5].
Methyl syringate (5-20 μM, 6 h) significantly increases AMPK phosphorylation in 3T3-L1 adipocytes at 10 or 20 μM dosage^[5].
Methyl syringate (1-20 μM, 6 d) does not affect adipocyte differentiation in differentiated 3T3-L1 preadipocytes, indicating it does not induce lipid accumulation^[5].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:

A549 under hypoxic conditions
Concentration:

1 mM
Incubation Time:

1 h
Result:

Inhibited hypoxia-induced COX-2 protein through TRPA1.
Was not affected by Sirt1 enzyme on its inhibition of hypoxia-induced COX-2 protein expression.
Was blocked by HC-030031 (HY-15064) (100 μM) on its inhibition of hypoxia-induced COX-2 protein expression under hypoxic conditions.

ELISA Assay^[4]

Cell Line:

A549 under hypoxic conditions
Concentration:

1 mM
Incubation Time:

1 h
Result:

Decreased A549 migration under hypoxic conditions by approximately 40%.
Blocked by HC-030031 (100 μM) on its inhibition of cell migration, suggesting that its effects on hypoxia-induced migration are mediated by TRPA1.
Decreased hypoxia-enhanced A549 invasion.

In Vivo

Methyl syringate (10 mg/kg, p.o., one single dose) significantly reduces the cumulative food intake 24 h after treatment in male ICR mice experienced fasting and allowed ad libitum access of food and water^[1].
Methyl syringate (0.1-10 mg/kg, p.o., one single dose) significantly decreases the food remaining in the stomach 28 h after treatment in male ICR mice ^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male ICR mice experienced fasting and allowed ad libitum access of food and water^[1]
Dosage:

0.1-10 mg/kg
Administration:

Oral gavage (p.o.)
Result:

Did not alter the body weight significantly compared to the control.
Delayed gastric emptying dose-dependently with around 70% reduction at 10 mg/kg compared to the control.
Increased plasma PYY levels 15 min after treatment but did not affect GLP-1 levels.

Chemical Information

CAS No. 884-35-5
Appearance Solid
Molecular Weight 212.20
Formula C₁₀H₁₂O₅
Color White to off-white
SMILES

O=C(OC)C1=CC(OC)=C(O)C(OC)=C1
Structure Classification
- Phenols
- Monophenols
Initial Source
- Animals
honey
Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Solvent & Solubility

In Vitro:

DMSO : 100 mg/mL (471.25 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

4.7125 mL

23.5627 mL

47.1254 mL

5 mM

0.9425 mL

4.7125 mL

9.4251 mL

10 mM

0.4713 mL

2.3563 mL

4.7125 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (11.78 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Kim MJ, et al. The TRPA1 agonist, methyl syringate suppresses food intake and gastric emptying. PLoS One. 2013 Aug 21;8(8):e71603. [Content Brief]

[2]. Rosado T, et al. Methyl syringate: an efficient phenolic mediator for bacterial and fungal laccases. Bioresour Technol. 2012 Nov;124:371-8. [Content Brief]

[3]. Joonwoo Park, et al., Methyl syringate, a TRPA1 agonist represses hypoxia-induced cyclooxygenase-2 in lung cancer cells, Phytomedicine, Volume 23, Issue 3, 2016, Pages 324-329, ISSN 0944-7113.

[4]. Ahn D, et al., Methyl Syringate Stimulates Glucose Uptake by Inhibiting Protein Tyrosine Phosphatases Relevant to Insulin Resistance. Life. 2023; 13(6):1372.

[5]. Tuberoso CI, et al. Methyl syringate: a chemical marker of asphodel (Asphodelus microcarpus Salzm. et Viv.) monofloral honey. J Agric Food Chem. 2009 May 13;57(9):3895-900. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	4.7125 mL	23.5627 mL	47.1254 mL	117.8134 mL
	5 mM	0.9425 mL	4.7125 mL	9.4251 mL	23.5627 mL
	10 mM	0.4713 mL	2.3563 mL	4.7125 mL	11.7813 mL
	15 mM	0.3142 mL	1.5708 mL	3.1417 mL	7.8542 mL
	20 mM	0.2356 mL	1.1781 mL	2.3563 mL	5.8907 mL
	25 mM	0.1885 mL	0.9425 mL	1.8850 mL	4.7125 mL
	30 mM	0.1571 mL	0.7854 mL	1.5708 mL	3.9271 mL
	40 mM	0.1178 mL	0.5891 mL	1.1781 mL	2.9453 mL
	50 mM	0.0943 mL	0.4713 mL	0.9425 mL	2.3563 mL
	60 mM	0.0785 mL	0.3927 mL	0.7854 mL	1.9636 mL
	80 mM	0.0589 mL	0.2945 mL	0.5891 mL	1.4727 mL
	100 mM	0.0471 mL	0.2356 mL	0.4713 mL	1.1781 mL

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Methyl syringate

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All TRP Channel Isoforms

All COX Isoforms

Biological Activity

Chemical Information

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Methyl syringate Related Classifications

Cat. No.: HY-W002116