ADP-β-S
ADP-β-S (Adenosine 5'-(β-thiodiphosphate)) is a non-hydrolyzable ADP analog and a P2Y12 receptor agonist. ADP-β-S activates the P2Y12 receptor in microglia, thereby triggering downstream inflammatory signaling pathways. ADP-β-S activates P2Y purinergic receptors in rat pancreatic β cells and enhances glucose-induced insulin secretion. ADP-β-S can be used in the research of diseases such as inflammation and diabetes.
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- CAS. Nr.: 35094-45-2
- Formel: C10H15N5O9P2S
- Molecular Weight:443.27
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
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Chemical Information
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Biologische Aktivität
Beschreibung
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| Oocyte | EC50 |
100 μM
Compound: ADPbetaS
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Antagonist activity against recombinant rat receptor P2X purinoceptor 2 (P2X2) at 10 uM, expressed in Xenopus oocytes
Antagonist activity against recombinant rat receptor P2X purinoceptor 2 (P2X2) at 10 uM, expressed in Xenopus oocytes
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[PMID: 12213051] |
| PC-12 | IC50 |
20 μM
Compound: 5
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Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis
Antioxidant activity in rat PC12 cells assessed as inhibition of Fe2+-induced reactive oxygen species formation after 1 hr by DCFH-DA dye-based fluorometric analysis
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[PMID: 26447940] |
In Vitro
ADP-β-S (0.2-2 mM; 30 min) enhances IL-1β production in primary cultured murine microglia via the P2Y12 receptor, with significant effects observed at 0.2 mM and 2 mM concentrations when paired with LPS or LPS plus ATP stimulation[1].
ADP-β-S (0.2 mM; 30 min) enhances LPS plus ATP-induced IL-1β production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) enhances LPS-induced IL-6 production in MG6 murine microglial cells via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) enhances LPS-induced IL-6 production in primary cultured murine microglia via the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) enhances LPS-induced IL-6 production in wild-type MG6 murine microglial cells, but not in P2ry12 KO MG6 cells, confirming dependence on the P2Y12 receptor[1].
ADP-β-S (0.2 mM; 24 h) potentiates LPS-induced NF-κB activation, including nuclear translocation and phosphorylation, in MG6 murine microglial cells[1].
ADP-β-S (trilithium) (0.2-2 mM; 30 min) activates caspase-1 at 2 mM and enhances LPS plus ATP-induced caspase-1 activation at 0.2 mM in MG6 murine microglial cells[1].
ADP-β-S (0.2 mM; 30 min) enhances LPS plus ATP-induced mitochondrial membrane potential disruption in MG6 murine microglial cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:primary cultured murine microglia
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Concentration:0.2 mM, 2 mM
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Incubation Time:30 min
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Result:Induced significant IL-1β release when paired with LPS at 2 mM, which was partially blocked by PSB0739.
Induced modest but significant IL-1β release when paired with LPS at 0.2 mM.
Significantly augmented ATP-induced IL-1β production when added alongside LPS plus 1 or 2 mM ATP at 0.2 mM, and this augmentation was suppressed by PSB0739.
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Cell Line:wild-type and P2ry12 KO MG6 immortalized murine microglial cells
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Concentration:0.2 mM
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Incubation Time:30 min
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Result:Significantly increased IL-1β production in wild-type MG6 cells stimulated with LPS plus ATP.
Had no effect on IL-1β production in P2ry12 KO MG6 cells under the same conditions.
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Cell Line:MG6 immortalized murine microglial cells
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Concentration:0.2 mM
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Incubation Time:24 h
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Result:Significantly increased LPS-induced IL-6 production in MG6 cells, and this increase was abrogated by the P2Y12 receptor antagonist PSB0739.
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Cell Line:primary cultured murine microglia
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Concentration:0.2 mM
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Incubation Time:24 h
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Result:Significantly increased LPS-induced IL-6 production in primary microglia, and this increase was reduced by the P2Y12 receptor antagonist PSB0739.
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Cell Line:wild-type and P2ry12 KO MG6 immortalized murine microglial cells
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Concentration:0.2 mM
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Incubation Time:24 h
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Result:Significantly increased LPS-induced IL-6 production in wild-type MG6 cells.
Had no effect on IL-6 production in P2ry12 KO MG6 cells under the same conditions.
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Cell Line:MG6 immortalized murine microglial cells
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Concentration:0.2 mM, 2 mM
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Incubation Time:30 min
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Result:Activated caspase-1 when paired with LPS at 2 mM, as shown by presence of cleaved caspase-1 in culture supernatants.
Strongly enhanced caspase-1 activation when paired with LPS plus 1 mM ATP at 0.2 mM compared to LPS plus ATP alone.
Chemical Information
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CAS. Nr. 35094-45-2
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Molecular Weight 443.27
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Formel C10H15N5O9P2S
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SMILES
O[C@@H]1[C@H](O)[C@@H](COP(O)(OP(S)(O)=O)=O)O[C@H]1N2C3=NC=NC(N)=C3N=C2
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Synonyms
Adenosine 5'-(β-thiodiphosphate)
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
[1]. Suzuki T, et al. Extracellular ADP augments microglial inflammasome and NF-κB activation via the P2Y12 receptor. Eur J Immunol. 2020;50(2):205-219. [Content Brief]
[2]. Bertrand G, et al. Adenosine-5'-O-(2-thiodiphosphate) is a potent agonist at P2 purinoceptors mediating insulin secretion from perfused rat pancreas. Br J Pharmacol. 1991;102(3):627-630. [Content Brief]
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
ADP-β-S Related Classifications
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