Cat. No.: HY-N1731

2′-Hydroxy-5′-methoxyacetophenone

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2'-Hydroxy-5'-methoxyacetophenone is an acetophenone derivative with acaricidal activities. 2'-Hydroxy-5'-methoxyacetophenone attenuates the inﬂammatory response via NF-κB signaling pathway. 2'-Hydroxy-5'-methoxyacetophenone exhibits significant inhibitory activity against α-amylase, collagenase and aldose reductase (AR) with IC₅₀s of 0.928, 3.264 and 20.046 μM, highlighting its potential in combating diabetes. 2'-Hydroxy-5'-methoxyacetophenone exhibits anti-ovarian cancer activity.

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Pureté: 99.67%
CAS No.: 705-15-7
Formule: C₉H₁₀O₃
Masse moléculaire:166.18

Stockage:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month

2′-Hydroxy-5′-methoxyacetophenone Chemical Structure

CAS No.: 705-15-7

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Activité biologique
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
Chemical Information
Solvant et solubilité
- In Vitro
- In Vivo
Pureté et documentation
Références
Help & FAQs
Classifications
Bioz

Activité biologique

Description

IC₅₀ & Target^[1]

NF-κB

COX-2

iNOS

Voir tous les produits spécifiques à Isoform NF-κB :

Voir tous les produits spécifiques à Isoform COX :

Voir tous les produits spécifiques à Isoform TNF Receptor :

Voir tous les produits spécifiques à Isoform NO Synthase :

Voir tous les produits spécifiques à Isoform Parasite :

Cellular Effect

Cell Line	Type	Value	Description	References
HCT-116	IC₅₀	43.31 μg/mL Compound: 48	Anticancer activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay Anticancer activity against human HCT-116 cells assessed as reduction in cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 33445154]

In Vitro

2'-Hydroxy-5'-methoxyacetophenone (10-200 μM, 24 h) exhibits anti-ovarian cancer activity against PA-1, Caov-3, and SK-OV-3 cell lines with IC₅₀s of 271, 326 and 405 µg/mL, respectively and has no cytotoxicity on has no cytotoxicity on BV-2 and RAW264.7 cells^[1].
2'-Hydroxy-5'-methoxyacetophenone (10-200 μM, 19-25 h) significantly decreases the LPS (HY-D1056)-induced NO, TNF-α, ROS, iNOS and COX-2 production and inhibits NF-κB signaling pathway in BV-2 and RAW264.7 cells^[1].
2'-Hydroxy-5'-methoxyacetophenone exhibits LD₅₀ values against D. farina, D. pteronyssinus and T. putrescentiae of 1.03, 1.13 and 1.78 μg/cm².

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

ELISA Assay^[1]

Cell Line:

BV-2 and RAW264.7 cells
Concentration:

10, 50, and 200 μM
Incubation Time:

Pre-treated for 1 h and then activated by LPS for 24 h
Result:

Inhibited the production of TNF-α in a dose-dependent manner.

Western Blot Analysis^[1]

Cell Line:

BV-2 and RAW264.7 cells
Concentration:

10, 50, and 200 μM
Incubation Time:

Pre-treated for 1 h and then activated by LPS for 18 or 24 h
Result:

Reduced the protein level of COX-2 and iNOS.
Restrained the LPS-induced NF-κB activation, the patterns of IκBα, NF-κB-p65, p-IκBα, and p-NF-κB p65.
Inhibited the activity of NF-κB in the nucleus.

Immunofluorescence^[1]

Cell Line:

BV-2 and RAW264.7 cells
Concentration:

200 μM
Incubation Time:

Pre-treated for 1 h and then activated by LPS for 24 h
Result:

Inhibited the translocation of NF-κB p65.

Chemical Information

CAS No. 705-15-7
Appearance Solid
Masse moléculaire 166.18
Formule C₉H₁₀O₃
Color Light yellow to green yellow
SMILES

CC(C1=CC(OC)=CC=C1O)=O
Livraison

Room temperature in continental US; may vary elsewhere.

Stockage

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvant et solubilité

In Vitro:

DMSO : 100 mg/mL (601.76 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

6.0176 mL

30.0879 mL

60.1757 mL

5 mM

1.2035 mL

6.0176 mL

12.0351 mL

10 mM

0.6018 mL

3.0088 mL

6.0176 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)

Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.

Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (15.04 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (280 KB)
SDS (615 KB)
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- Portuguese - PT (615 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Zhou J, et al. Discovery of 2-Ethoxy-5-isobutyramido-N-1-substituted Benzamide Derivatives as Selective Kv2.1 Inhibitors with In Vivo Neuroprotective Effects. J Med Chem. 2024 Jan 11;67(1):213-233. [Content Brief]

[2]. Kim MG, Yang JY, Lee HS. Acaricidal potentials of active properties isolated from Cynanchum paniculatum and acaricidal changes by introducing functional radicals. J Agric Food Chem. 2013 Aug 7;61(31):7568-73. [Content Brief]

[3]. Li Wang et, al. Anti-ovarian cancer and collagenase, alpha amylase, and aldose reductase inhibition properties of 2′-Hydroxy-5′-methoxyacetophenone with molecular modeling studies. 2021 Sep.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	6.0176 mL	30.0879 mL	60.1757 mL	150.4393 mL
	5 mM	1.2035 mL	6.0176 mL	12.0351 mL	30.0879 mL
	10 mM	0.6018 mL	3.0088 mL	6.0176 mL	15.0439 mL
	15 mM	0.4012 mL	2.0059 mL	4.0117 mL	10.0293 mL
	20 mM	0.3009 mL	1.5044 mL	3.0088 mL	7.5220 mL
	25 mM	0.2407 mL	1.2035 mL	2.4070 mL	6.0176 mL
	30 mM	0.2006 mL	1.0029 mL	2.0059 mL	5.0146 mL
	40 mM	0.1504 mL	0.7522 mL	1.5044 mL	3.7610 mL
	50 mM	0.1204 mL	0.6018 mL	1.2035 mL	3.0088 mL
	60 mM	0.1003 mL	0.5015 mL	1.0029 mL	2.5073 mL
	80 mM	0.0752 mL	0.3761 mL	0.7522 mL	1.8805 mL
	100 mM	0.0602 mL	0.3009 mL	0.6018 mL	1.5044 mL

Keywords

2′-Hydroxy-5′-methoxyacetophenone

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2′-Hydroxy-5′-methoxyacetophenone

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Voir tous les produits spécifiques à Isoform NF-κB

Voir tous les produits spécifiques à Isoform COX

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Voir tous les produits spécifiques à Isoform NO Synthase

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Activité biologique

Chemical Information

Solvant et solubilité

Pureté et documentation

Références

Help & FAQs

Complete Stock Solution Preparation Table

2′-Hydroxy-5′-methoxyacetophenone Related Classifications

HY-N1731 Related Classifications