Cat. No.: HY-N2443

Tribuloside

2 Cited Publications

Customer Review

Based on 2 publication(s) in Google Scholar

Tribuloside is a flavonoid and PDE4 inhibitor (IC₅₀: 6 μM). Tribuloside exhibits antibacterial and antioxidant activities, capable of scavenging DPPH free radicals. Tribuloside also promotes melanogenesis. Tribuloside can be used in the research of hypopigmentary diseases, sun protection, and inflammatory diseases such as acute lung injury.

For research use only. We do not sell to patients.

Purity: 98.95%
CAS No.: 22153-44-2
Formula: C₃₀H₂₆O₁₃
Molecular Weight:594.52

Storage:

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Tribuloside Chemical Structure

CAS No.: 22153-44-2

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Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- Cellular Effect
- In Vitro
- In Vivo
Chemical Information
Publications (2)
Solvent & Solubility
- In Vitro
- In Vivo
Purity & Documentation
References
Help & FAQs
Classifications
Bioz

Biological Activity

Description

IC₅₀ & Target^[2]^[3]

PDE4

6 μM (IC₅₀)

IL-1β

IL-6

All Phosphodiesterase (PDE) Isoforms :

All PKA Isoforms :

All TRP Channel Isoforms :

All Interleukin Related Isoforms :

Cellular Effect

Cell Line	Type	Value	Description	References
Ehrlich	IC₅₀	69.5 μM Compound: 11	Cytotoxicity against mouse Ehrlich carcinoma cells Cytotoxicity against mouse Ehrlich carcinoma cells	[PMID: 19254846]
HepG2	EC₅₀	0.155 μM Compound: Tiliroside	Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs Increase in glucose consumption in insulin-resistant human HepG2 cells after 24 hrs	[PMID: 21856048]
Monocyte	IC₅₀	5.2 μM Compound: tiliroside	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]
Monocyte	IC₅₀	5.2 x 10^-6 M Compound: tiliroside	Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs Inhibition of procoagulant activity in monocyte from human blood assessed as counteraction of IL1-induced tissue factor expression after 18 hrs	[PMID: 8882428]

In Vitro

Tribuloside exhibits antimycobacterial activity against non-pathogenic Mycobacterium madagascariense and Mycobacterium indicus pranii with MIC values of 0.8-1.6 mg/mL^[1].
Tribuloside (0-20 μM; 48 h) significantly increases melanogenesis, promotes dendrite formation, and enhances melanosome maturation and transport in human epidermal melanocytes, which is associated with the PDE/cAMP/PKA pathway^[2].
Tribuloside (0-20 μM; 0-48 h) upregulates the expression of proteins related to melanogenesis and melanosome transport in human epidermal melanocytes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[2]

Cell Line:

Human epidermal melanocytes (HEMCs)
Concentration:

0, 5, 10 and 20 μM
Incubation Time:

0, 4, 12, 24 and 48 h
Result:

Increased the levels of PMEL-17, tyrosinase, TRP-1, TRP-2 and MITF in a time- and dose-dependent manner.
Raised the levels of Rab27a, Rab17 and Cdc42 in a time- and dose-dependent manner.

In Vivo

Tribuloside (5-20 μM; dissolved in embryo medium; single addition; 35-60 h) reverses hypopigmentation and increases melanin content in zebrafish embryo depigmentation models induced by 1-Phenyl-2-thiourea^[2].
Tribuloside (10 mg/kg; intraperitoneal injection; single dose) improves LPS (HY-D1056)-induced acute lung injury in mice^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

Male mice aged 6 weeks old (30-40 g) treated LPS (HY-D1056)^[3]
Dosage:

10 mg/kg
Administration:

Intraperitoneal injection; single dose
Result:

Significantly alleviated lung tissue inflammation and fibrosis, repaired damaged alveolar structure, increased the number of alveoli and the percentage of alveolar area.
Reduced the expression levels of inflammatory cytokines IL-1β, TNF-α, and IL-6 in bronchoalveolar lavage fluid.

Chemical Information

CAS No. 22153-44-2
Appearance Solid
Molecular Weight 594.52
Formula C₃₀H₂₆O₁₃
Color Light yellow to yellow
SMILES

O=C1C(O[C@@H]2O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)COC(/C=C/C3=CC=C(O)C=C3)=O)=C(C4=CC=C(O)C=C4)OC5=CC(O)=CC(O)=C51
Structure Classification
- Flavonoids
- Flavonols
- Phenols
- Polyphenols
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

-20°C, protect from light

* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)

Publications (2)

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Journal Impact Factor

J Ethnopharmacol

Tribuloside acts on the PDE/cAMP/PKA pathway to enhance melanogenesis, melanocyte dendricity and melanosome transport. [Abstract]

2024 Apr 6:323:117673. PMID: 38158096
SSRN

2026 Mar 31.

Solvent & Solubility

In Vitro:

DMSO : 125 mg/mL (210.25 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

1.6820 mL

8.4101 mL

16.8203 mL

5 mM

0.3364 mL

1.6820 mL

3.3641 mL

10 mM

0.1682 mL

0.8410 mL

1.6820 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Dilution Calculator

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (4.21 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (280 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Christopher R, et al. A new cinnamoylglycoflavonoid, antimycobacterial and antioxidant constituents from Heritiera littoralis leaf extracts. Nat Prod Res. 2014;28(6):351-8. [Content Brief]

[2]. Cao Y, et al. Tribuloside acts on the PDE/cAMP/PKA pathway to enhance melanogenesis, melanocyte dendricity and melanosome transport. J Ethnopharmacol. 2024 Apr 6;323:117673. [Content Brief]

[3]. Yang Z, et al. Tribuloside: Mechanisms and Efficacy in Treating Acute Lung Injury Revealed by Network Pharmacology and Experimental Validation. Dose Response. 2024 May 8;22(2):15593258241251594. [Content Brief]

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	1.6820 mL	8.4101 mL	16.8203 mL	42.0507 mL
	5 mM	0.3364 mL	1.6820 mL	3.3641 mL	8.4101 mL
	10 mM	0.1682 mL	0.8410 mL	1.6820 mL	4.2051 mL
	15 mM	0.1121 mL	0.5607 mL	1.1214 mL	2.8034 mL
	20 mM	0.0841 mL	0.4205 mL	0.8410 mL	2.1025 mL
	25 mM	0.0673 mL	0.3364 mL	0.6728 mL	1.6820 mL
	30 mM	0.0561 mL	0.2803 mL	0.5607 mL	1.4017 mL
	40 mM	0.0421 mL	0.2103 mL	0.4205 mL	1.0513 mL
	50 mM	0.0336 mL	0.1682 mL	0.3364 mL	0.8410 mL
	60 mM	0.0280 mL	0.1402 mL	0.2803 mL	0.7008 mL
	80 mM	0.0210 mL	0.1051 mL	0.2103 mL	0.5256 mL
	100 mM	0.0168 mL	0.0841 mL	0.1682 mL	0.4205 mL

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Tribuloside

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Publications Citing Use of MedChemExpress (MCE) Tribuloside

All Phosphodiesterase (PDE) Isoforms

All PKA Isoforms

All TRP Channel Isoforms

All Interleukin Related Isoforms

Biological Activity

Chemical Information

Publications (2)

Solvent & Solubility

Purity & Documentation

References

Help & FAQs

Complete Stock Solution Preparation Table

Tribuloside Related Classifications

HY-N2443 Related Classifications