Wortmannin
Based on 179 publication(s) in Google Scholar
Wortmannin (SL-2052; KY-12420) is a potent, selective and irreversible PI3K inhibitor with an IC50 of 3 nM. Wortmannin also blocks autophagy formation, and potently inhibits Polo-like kinase 1 (PlK1) and Plk3 with IC50s of 5.8 and 48 nM, respectively.
Para uso exclusivo en investigación. No vendemos a pacientes.
- Pureza: 99.85%
- No. CAS: 19545-26-7
- Fòrmula: C23H24O8
- Peso molecular:428.43
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year
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Publications Citing Use of MedChemExpress (MCE) Wortmannin
More- Signal Transduct Target Ther. 2024 Mar 9;9(1):65. [Abstract]
- Signal Transduct Target Ther. 2022 Dec 9;7(1):388. [Abstract]
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- Autophagy. 2021 Nov;17(11):3592-3606. [Abstract]
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- Nat Commun. 2025 Dec 2;16(1):10831. [Abstract]
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- Nat Commun. 2024 Jan 26;15(1):759. [Abstract]
- Nat Commun. 2021 Jun 8;12(1):3444. [Abstract]
- ACS Nano. 2025 Dec 26. [Abstract]
- ACS Nano. 2020 Apr 28;14(4):4890-4904. [Abstract]
- BMEmat. 2026 Feb 11.
- J Adv Res. 2023 May:47:151-162. [Abstract]
- Redox Biol. 2023 Sep:65:102825. [Abstract]
- Redox Biol. 2023 Jun:62:102702. [Abstract]
- Redox Biol. 2022 May 17;53:102339. [Abstract]
- Redox Biol. 2022 May 10;53:102323. [Abstract]
- Redox Biol. 2021 Oct;46:102098. [Abstract]
- Sci Transl Med. 2026 May 20;18(850):eadj4902. [Abstract]
- Sci Transl Med. 2021 Jan 27;13(578):eaba7308. [Abstract]
- J Nanobiotechnology. 2025 Aug 26;23(1):590. [Abstract]
- Theranostics. 2021 Mar 11;11(11):5279-5295. [Abstract]
- Genes Dis. 2025 Nov 6.
- Acta Pharm Sin B. 2024 Mar;14(3):1166-1186. [Abstract]
- Adv Sci (Weinh). 2026 Apr;13(24):e21626. [Abstract]
- Adv Sci (Weinh). 2025 May;12(20):e2504647. [Abstract]
- Adv Sci (Weinh). 2023 Jun;10(17):e2207017. [Abstract]
- Adv Sci (Weinh). 2023 Feb;10(5):e2203884. [Abstract]
- Cell Rep Med. 2025 Dec 30:102531. [Abstract]
- Cell Rep Med. 2024 Feb 20;5(2):101357. [Abstract]
- Sci Adv. 2022 Jan 21;8(3):eabh2635. [Abstract]
- Cell Death Differ. 2023 Jan;30(1):168-183. [Abstract]
- Carbohydr Polym. 2025 Jan 15;348(Pt A):122854. [Abstract]
- Chem Eng J. 2025 Jul 1.
- J Control Release. 2026 May 10:393:114833. [Abstract]
- J Control Release. 2025 Jan 8:379:45-58. [Abstract]
- Cell Death Dis. 2025 Apr 21;16(1):322. [Abstract]
- Cell Mol Biol Lett. 2024 Feb 2;29(1):22. [Abstract]
- Cell Mol Biol Lett. 2023 Oct 24;28(1):85. [Abstract]
- Cell Death Dis. 2022 Jul 15;13(7):615. [Abstract]
- Cell Death Dis. 2022 Feb 17;13(2):158. [Abstract]
- Cell Death Dis. 2019 Aug 5;10(8):583. [Abstract]
- Cancer Lett. 2022 Aug 10:541:215752. [Abstract]
- Cancer Lett. 2021 Oct 28;519:2-19. [Abstract]
- Small. 2020 Nov;16(44):e2004172. [Abstract]
- J Exp Med. 2022 May 2;219(5):e20212414. [Abstract]
- Phytomedicine. 2025 Jan:136:156314. [Abstract]
- Phytomedicine. 2024 Jul 25:130:155726. [Abstract]
- J Pharm Anal. 2025 May;15(5):101251. [Abstract]
- Mater Today Bio. 2025 Apr 17:32:101778. [Abstract]
- Acta Pharmacol Sin. 2025 Sep 1. [Abstract]
- Acta Biomater. 2025 Jan 24:193:498-513. [Abstract]
- Cell Death Discov. 2024 Jul 19;10(1):329. [Abstract]
- Acta Pharmacol Sin. 2022 Apr;43(4):781-787. [Abstract]
- Food Chem. 2021 Nov 30:363:130323. [Abstract]
- Mol Psychiatry. 2021 Nov;26(11):6451-6468. [Abstract]
- Diabetes. 2026 Feb 13:db250567. [Abstract]
- Cell Chem Biol. 2026 Apr 16;33(4):523-537.e7. [Abstract]
- Cell Chem Biol. 2022 Jul 21;29(7):1113-1125.e6. [Abstract]
- Int J Biol Macromol. 2024 May 1;269(Pt 1):132018. [Abstract]
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- Microbiol Res. 2024 Dec:289:127927. [Abstract]
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- Clin Transl Med. 2022 Feb;12(2):e747. [Abstract]
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- Br J Cancer. 2023 Mar;128(7):1344-1359. [Abstract]
- Cell Rep. 2025 May 3;44(5):115672. [Abstract]
- Cell Prolif. 2024 Nov;57(11):e13700. [Abstract]
- Food Sci Hum Wellness. 2026 Apr 29.
- Anal Chem. 2020 Jan 21;92(2):2103-2111. [Abstract]
- Cancer Cell Int. 2023 Apr 16;23(1):68. [Abstract]
- Front Immunol. 2021 Nov 24;12:786602. [Abstract]
- NPJ Regen Med. 2021 Sep 6;6(1):51. [Abstract]
- Antioxid Redox Signal. 2024 Apr;40(10-12):598-615. [Abstract]
- Ecotoxicol Environ Saf. 2025 Jan 1:289:117448. [Abstract]
- Biochem Pharmacol. 2026 Mar:245:117628. [Abstract]
- Cell Mol Life Sci. 2024 May 30;81(1):244. [Abstract]
- Cell Mol Life Sci. 2018 Jul;75(14):2627-2641. [Abstract]
- Precis Clin Med. 2026 Mar 14;9(2):pbag009. [Abstract]
- Life Sci. 2025 Nov 1:380:123934. [Abstract]
- Life Sci. 2020 Dec 15:263:118713. [Abstract]
- Food Biosci. 2025 Jun.
- EMBO Rep. 2024 Oct;25(10):4488-4514. [Abstract]
- Nutrients. 2024 Nov 13;16(22):3869. [Abstract]
- Cell Biol Toxicol. 2025 Jan 28;41(1):35. [Abstract]
- Mar Drugs. 2024 Apr 13;22(4):174. [Abstract]
- Int J Mol Sci. 2025 Jul 13;26(14):6720. [Abstract]
- Int J Mol Sci. 2025 Mar 29;26(7):3172. [Abstract]
- Int Immunopharmacol. 2024 Jan 5:126:111237. [Abstract]
- Int Immunopharmacol. 2023 Sep:122:110605. [Abstract]
- Int Immunopharmacol. 2022 Oct:111:108806. [Abstract]
- Glia. 2023 Jul;71(7):1648-1666. [Abstract]
- Arthritis Res Ther. 2018 Feb 7;20(1):20. [Abstract]
- Molecules. 2023 Jan 18;28(3):968. [Abstract]
- Molecules. 2020 Apr 23;25(8):1980. [Abstract]
- Mol Med Rep. 2020 Feb;21(2):597-606. [Abstract]
- J Mol Med (Berl). 2019 Aug;97(8):1183-1193. [Abstract]
- Sci Rep. 2026 Jan 3;16(1):3374. [Abstract]
- Cancer Sci. 2021 Apr;112(4):1624-1632. [Abstract]
- PLoS Pathog. 2020 Mar 24;16(3):e1008429. [Abstract]
- Cell Signal. 2025 Jul:131:111706. [Abstract]
- J Cell Mol Med. 2021 Jun;25(11):5238-5249. [Abstract]
- Oncol Rep. 2019 Aug;42(2):479-494. [Abstract]
- Plant Sci. 2023 Nov:336:111859. [Abstract]
- Front Mol Biosci. 2022 Jul 22:9:952608. [Abstract]
- FASEB J. 2025 Aug 31;39(16):e70901. [Abstract]
- FASEB J. 2025 Jan 31;39(2):e70339. [Abstract]
- FASEB J. 2023 Apr;37(4):e22880. [Abstract]
- Front Physiol. 2021 May 13:12:648399. [Abstract]
- Fish Shellfish Immunol. 2022 Oct:129:22-29. [Abstract]
- J Virol. 2026 Jun 23;100(6):e0206125. [Abstract]
- J Virol. 2025 Aug 11:e0077125. [Abstract]
- J Virol. 2021 Nov 23;95(24):e0134521. [Abstract]
- J Virol. 2021 Jul 26;95(16):e0076021. [Abstract]
- Chem Res Toxicol. 2019 Dec 16;32(12):2479-2487. [Abstract]
- Naunyn Schmiedebergs Arch Pharmacol. 2026 Apr 18. [Abstract]
- J Cell Physiol. 2025 Nov;240(11):e70108. [Abstract]
- Virol J. 2025 Apr 17;22(1):103. [Abstract]
- J Funct Foods. August 2022, 105199.
- J Funct Foods. 2021, 104585.
- Viruses. 2024 Feb 5;16(2):256. [Abstract]
- Viruses. 2023 Aug 24;15(9):1805. [Abstract]
- Mol Immunol. 2021 Aug:136:55-64. [Abstract]
- Int J Med Sci. 2020 Sep 28;17(17):2751-2762. [Abstract]
- Toxicol Appl Pharmacol. 2024 Feb:483:116836. [Abstract]
- PLoS Negl Trop Dis. 2023 May 4;17(5):e0011281. [Abstract]
- Cardiovasc Drugs Ther. 2025 May 22. [Abstract]
- Arch Biochem Biophys. 2023 Jul 15:743:109646. [Abstract]
- J Reprod Immunol. 2023 Feb:155:103766. [Abstract]
- J Cosmet Dermatol. 2022 Mar;21(3):1163-1170. [Abstract]
- Arch Biochem Biophys. 2021 Oct 30:711:109015. [Abstract]
- Diabetes Metab Syndr Obes. 2019 Nov 4:12:2289-2302. [Abstract]
- J Leukoc Biol. 2026 Jun 27:qiag090. [Abstract]
- Brain Sci. 2021 Nov 11;11(11):1487. [Abstract]
- Food Chem Toxicol. 2019 Dec:134:110830. [Abstract]
- Brain Res. 2019 Oct 15:1721:146337. [Abstract]
- Polymers. 2023 Mar 28;15(7):1681. [Abstract]
- Vet Microbiol. 2026 Jul 10:320:111134.
- Vet Microbiol. 2025 May:304:110496. [Abstract]
- Vet Microbiol. 2022 Aug:271:109488. [Abstract]
- Vet Parasitol. 2026 May:344:110769. [Abstract]
- Virology. 2023 Aug:585:21-31. [Abstract]
- Int J Pept Res Ther. 2025 May 22.
- Clin Cosmet Investig Dermatol. 2022 Aug 2;15:1499-1508. [Abstract]
- J Cardiovasc Pharmacol. 2021 Jun 1;77(6):814-821. [Abstract]
- Clin Exp Pharmacol Physiol. 2020 Dec;47(12):1953-1964. [Abstract]
- Biochem Biophys Res Commun. 2017 Aug 5;489(4):484-489. [Abstract]
- Int J Ophthalmol. 2025 Jan 18;18(1):28-38. [Abstract]
- Nat Prod Commun. 2023 Dec 20.
- Biosci Biotechnol Biochem. 2023 Nov 21;87(12):1485-1494. [Abstract]
- J Vis Exp. 2026 Feb 13:(228). [Abstract]
- Reproductive Medicine. 2022, 3(2), 112-126.
- Stem Cell Res. 2023 Feb:66:103010. [Abstract]
- bioRxiv. 2026 Jul 2:2026.06.25.734564.
- bioRxiv. 2026 May 19:2026.05.17.725791. [Abstract]
- Cell Biomater. 2026 Mar 6.
- bioRxiv. 2025 Sep 2.
- SSRN. 2025 Aug 13.
- Research Square Preprint. 2024 Dec 18.
- Heliyon. 2024 Aug 6;10(16):e35832. [Abstract]
- Environ Toxicol. 2024 Jul;39(7):3920-3929. [Abstract]
- biorxiv. 2024 May 27.
- SSRN. 2024 May 15.
- Research Square Preprint. 2024 Feb 13.
- Elife. 2024 Feb 20:13:e85985. [Abstract]
- Environ Toxicol. 2023 Nov;38(11):2621-2631. [Abstract]
- bioRxiv. 2023 Feb 14.
- SSRN. 2023 Feb 2.
- Environ Toxicol. 2023 Feb;38(2):343-358. [Abstract]
- Front Med. 2022 Apr;16(2):227-239. [Abstract]
- Ann Transl Med. 2020 Mar;8(6):333. [Abstract]
- Ann Transl Med. 2020 Mar;8(5):219. [Abstract]
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Actividad biológica
Descripciòn
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PI3K 3 nM (IC50) |
DNA-PK 16 nM (IC50) |
PLK3 48 nM (IC50) |
ATM 150 nM (IC50) |
ATR 1.8 μM (IC50) |
MLCK 200 nM (IC50) |
Autophagy |
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| A549 | IC50 |
0.22 μM
Compound: wortmannin
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Inhibition of Plk3 in human A549 cells assessed as casein substrate phosphorylation by Western blot
Inhibition of Plk3 in human A549 cells assessed as casein substrate phosphorylation by Western blot
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[PMID: 17135248] |
| A549 | IC50 |
11 nM
Compound: Wortmannin
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Inhibition of PI3K in human A549 cell lysate preincubated with compound for 5 mins followed by [gamma-32p]-ATP addition measured after 20 mins by immunoprecipitation based liquid scintillation spectroscopic analysis
Inhibition of PI3K in human A549 cell lysate preincubated with compound for 5 mins followed by [gamma-32p]-ATP addition measured after 20 mins by immunoprecipitation based liquid scintillation spectroscopic analysis
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[PMID: 16622124] |
| A549 | IC50 |
11.4 μM
Compound: 1, wortmannin, Wm
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Antiproliferative activity against human A549 cells after 48 hrs by SRB method
Antiproliferative activity against human A549 cells after 48 hrs by SRB method
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[PMID: 18630894] |
| A549 | IC50 |
4.28 ng/mL
Compound: wortmannin
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Cytotoxicity against human A549 cells after 24 hrs by propidium iodide assay
Cytotoxicity against human A549 cells after 24 hrs by propidium iodide assay
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[PMID: 19138858] |
| A549 | IC50 |
4.28 ng/mL
Compound: wortmannin
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Cytotoxicity against human A549 cells after 24 hrs by YO-PRO-1 assay
Cytotoxicity against human A549 cells after 24 hrs by YO-PRO-1 assay
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[PMID: 19138858] |
| HeLa | IC50 |
49 nM
Compound: wortmannin
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Inhibition of AX-7503 binding to recombinant Plk3 expressed in HeLa cells by Western blot
Inhibition of AX-7503 binding to recombinant Plk3 expressed in HeLa cells by Western blot
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[PMID: 17135248] |
| MCF7 | IC50 |
>100 μM
Compound: Wortmannin
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Cytotoxicity against human MCF7 cells after 72 hrs by MTS/PMS assay
Cytotoxicity against human MCF7 cells after 72 hrs by MTS/PMS assay
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[PMID: 23040731] |
| Neutrophil | IC50 |
19 nM
Compound: Wortmannin
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Inhibition of fMLP-induced superoxide production in human neutrophils
Inhibition of fMLP-induced superoxide production in human neutrophils
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[PMID: 17095227] |
| Sf9 | IC50 |
0.012 μM
Compound: (+)-2
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Inhibition of human PI3Kalpha expressed in Sf9 cells by fluorescent polarization assay
Inhibition of human PI3Kalpha expressed in Sf9 cells by fluorescent polarization assay
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[PMID: 21121631] |
In Vitro
Wortmannin (0-100 nM; 24-72 hours) inhibits the proliferation of K562 cells in a time- and dose-dependent manner. The IC50 values at 24 hour, 48 hour, and 72 hour are 25±0.10 nM, 12.5±0.08 nM, and 6.25±0.11 nM, respectively[4]. Wortmannin prevents nuclear entry of YAP[6].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:K562 cells
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Concentration:0, 6.25, 12.5, 25, 50 and 100 nM
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Incubation Time:0, 24, 48 and 72 hours
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Result:Inhibited the K562 cells proliferation. The IC50 value at 24 hour, 48 hour, and 72 hour was 25±0.10 nM, 12.5±0.08 nM, and 6.25±0.11 nM.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Scid mice with the murine C3H mammary tumor or human MCF-7 breast cancer xenograft[5]
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Dosage:1 mg/kg and 1.5 mg/kg
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Administration:Oral gavage; daily; one group 1 mg/kg for 14 days; second group 1.5 mg/kg for 5 days then 1.0 mg/kg for 9 days.
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Result:The growth rate of the treated tumors was significantly slower during drug administration than that of nontreated tumors.
Chemical Information
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No. CAS 19545-26-7
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Appearance Solid
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Peso molecular 428.43
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Fòrmula C23H24O8
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Color White to yellow
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SMILES
O=C1C([C@@](CC2)([H])[C@@]3(C)C2=O)=C([C@H](OC(C)=O)C3)[C@]4(C)C5=C1OC=C5C(O[C@@H]4COC)=O
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Synonyms
SL-2052; KY-12420
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Structure Classification
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Initial Source
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 2 years -20°C 1 year
Publications (179)
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Journal Impact Factor
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Most Recent
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Signal Transduct Target Ther
Tet methylcytosine dioxygenase 2 (TET2) deficiency elicits EGFR-TKI (tyrosine kinase inhibitors) resistance in non-small cell lung cancer. [Abstract]2024 Mar 9;9(1):65. PMID: 38461173 -
Signal Transduct Target Ther
2022 Dec 9;7(1):388. PMID: 36481655 -
Nat Immunol
2024 Apr;25(4):659-670. PMID: 38499799 -
Bioact Mater
2025 Aug 6:53:801-818. PMID: 40809511 -
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Autophagy
WNT2B impairs endosomal trafficking via WASHC5 to inhibit autophagy: a novel non-secretory WNT pathway. [Abstract]2026 Jun 3:1-31. PMID: 42233622 -
Autophagy
Iterative design leads to a smart probe capable of quantifying autophagic flux with switchable fluorescence via engaging MAP1LC3/LC3. [Abstract]2026 May 20:1-18. PMID: 42163447 -
Autophagy
The mammalian actin elongation factor ENAH/MENA contributes to autophagosome formation via its actin regulatory function. [Abstract]2024 Aug;20(8):1798-1814. PMID: 38705725 -
Autophagy
Extracellular vesicles originating from autophagy mediate an antibody-resistant spread of classical swine fever virus in cell culture. [Abstract]2022 Jun;18(6):1433-1449. PMID: 34740307 -
Autophagy
Cannabidiol inhibits human glioma by induction of lethal mitophagy through activating TRPV4. [Abstract]2021 Nov;17(11):3592-3606. PMID: 33629929 -
Nat Commun
African swine fever virus B66L drives extracellular mitochondrial release to promote systemic inflammation in mice. [Abstract]2026 May 27. PMID: 42203763 -
Nat Commun
Human pluripotent stem cell-derived skin organoids enabled pathophysiological model of Mycobacterium tuberculosis infection. [Abstract]2025 Dec 2;16(1):10831. PMID: 41331115 -
Nat Commun
2025 Jul 18;16(1):6621. PMID: 40681515 -
Nat Commun
2024 Jan 26;15(1):759. PMID: 38272905 -
Nat Commun
2021 Jun 8;12(1):3444. PMID: 34103528 -
ACS Nano
Redox-Responsive Peptide Coacervates for Enhanced mRNA Delivery and Intracellular Release. [Abstract]2025 Dec 26. PMID: 41451635 -
ACS Nano
Enzyme-Triggered Transcytosis of Dendrimer-Drug Conjugate for Deep Penetration into Pancreatic Tumors. [Abstract]2020 Apr 28;14(4):4890-4904. PMID: 32286784 -
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J Adv Res
Paeonol protects against doxorubicin-induced cardiotoxicity by promoting Mfn2-mediated mitochondrial fusion through activating the PKCε-Stat3 pathway. [Abstract]2023 May:47:151-162. PMID: 35842187 -
Redox Biol
2023 Sep:65:102825. PMID: 37531930 -
Redox Biol
BNP protects against diabetic cardiomyopathy by promoting Opa1-mediated mitochondrial fusion via activating the PKG-STAT3 pathway. [Abstract]2023 Jun:62:102702. PMID: 37116257 -
Redox Biol
ROS generation attenuates the anti-cancer effect of CPX on cervical cancer cells by inducing autophagy and inhibiting glycophagy. [Abstract]2022 May 17;53:102339. PMID: 35636017 -
Redox Biol
TIGAR alleviates oxidative stress in brain with extended ischemia via a pentose phosphate pathway-independent manner. [Abstract]2022 May 10;53:102323. PMID: 35576689 -
Redox Biol
Paeonol promotes Opa1-mediated mitochondrial fusion via activating the CK2α-Stat3 pathway in diabetic cardiomyopathy. [Abstract]2021 Oct;46:102098. PMID: 34418601 -
Sci Transl Med
Real-time in vivo analysis of murine β cells reveals autophagic flux defects before onset of autoimmune diabetes. [Abstract]2026 May 20;18(850):eadj4902. PMID: 42160447 -
Sci Transl Med
A chimeric antigen receptor with antigen-independent OX40 signaling mediates potent antitumor activity. [Abstract]2021 Jan 27;13(578):eaba7308. PMID: 33504651 -
J Nanobiotechnology
Sparking angiogenesis by carbon monoxide-rich gold nanoparticles obtained by pulsed laser driven CO2 reduction reaction. [Abstract]2025 Aug 26;23(1):590. PMID: 40859339 -
Theranostics
A novel small-molecule PROTAC selectively promotes tau clearance to improve cognitive functions in Alzheimer-like models. [Abstract]2021 Mar 11;11(11):5279-5295. PMID: 33859747 -
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Acta Pharm Sin B
Extracellular vesicles from human urine-derived stem cells delay aging through the transfer of PLAU and TIMP1. [Abstract]2024 Mar;14(3):1166-1186. PMID: 38487008 -
Adv Sci (Weinh)
Coronavirus Nsp3 Hijacks CLTC to Modulate Autophagosome Nucleation for Promoting DMV Formation and Viral Replication. [Abstract]2026 Apr;13(24):e21626. PMID: 41721606 -
Adv Sci (Weinh)
Drug Screening of Primary Human Endometriotic Cells Based on Micro-Encapsulating Microfluidic Chip. [Abstract]2025 May;12(20):e2504647. PMID: 40289897 -
Adv Sci (Weinh)
CCR7 Mediated Mimetic Dendritic Cell Vaccine Homing in Lymph Node for Head and Neck Squamous Cell Carcinoma Therapy. [Abstract]2023 Jun;10(17):e2207017. PMID: 37092579 -
Adv Sci (Weinh)
RNF126-Mediated MRE11 Ubiquitination Activates the DNA Damage Response and Confers Resistance of Triple-Negative Breast Cancer to Radiotherapy. [Abstract]2023 Feb;10(5):e2203884. PMID: 36563124 -
Cell Rep Med
Discovery, delineation, and therapeutic targeting of a hyper-translation pathway driving cytokine release syndrome. [Abstract]2025 Dec 30:102531. PMID: 41475340 -
Cell Rep Med
Benzosceptrin C induces lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting DHHC3. [Abstract]2024 Feb 20;5(2):101357. PMID: 38237597 -
Sci Adv
Fasting improves therapeutic response in hepatocellular carcinoma through p53-dependent metabolic synergism. [Abstract]2022 Jan 21;8(3):eabh2635. PMID: 35061544 -
Cell Death Differ
FAAH served a key membrane-anchoring and stabilizing role for NLRP3 protein independently of the endocannabinoid system. [Abstract]2023 Jan;30(1):168-183. PMID: 36104448 -
Carbohydr Polym
Dextran-based nanodrugs with mitochondrial targeting/glutathione depleting synergy for enhanced photodynamic therapy. [Abstract]2025 Jan 15;348(Pt A):122854. PMID: 39562123 -
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J Control Release
Spore-inspired surface engineering enhances macrophage phagocytosis for inhalation therapy of acute lung injury. [Abstract]2026 May 10:393:114833. PMID: 41850404 -
J Control Release
Combating cisplatin-resistant lung cancer using a coiled-coil lipopeptides modified membrane fused drug delivery system. [Abstract]2025 Jan 8:379:45-58. PMID: 39756686 -
Cell Death Dis
2025 Apr 21;16(1):322. PMID: 40258807 -
Cell Mol Biol Lett
IP3R1-mediated MAMs formation contributes to mechanical trauma-induced hepatic injury and the protective effect of melatonin. [Abstract]2024 Feb 2;29(1):22. PMID: 38308199 -
Cell Mol Biol Lett
Inhibition of SQSTM1 S403 phosphorylation facilitates the aggresome formation of ubiquitinated proteins during proteasome dysfunction. [Abstract]2023 Oct 24;28(1):85. PMID: 37872526 -
Cell Death Dis
Autophagic sequestration of SQSTM1 disrupts the aggresome formation of ubiquitinated proteins during proteasome inhibition. [Abstract]2022 Jul 15;13(7):615. PMID: 35840557 -
Cell Death Dis
Targeting CCL2-CCR4 axis suppress cell migration of head and neck squamous cell carcinoma. [Abstract]2022 Feb 17;13(2):158. PMID: 35177591 -
Cell Death Dis
2019 Aug 5;10(8):583. PMID: 31378785 -
Cancer Lett
2022 Aug 10:541:215752. PMID: 35644286 -
Cancer Lett
UM-6 induces autophagy and apoptosis via the Hippo-YAP signaling pathway in cervical cancer. [Abstract]2021 Oct 28;519:2-19. PMID: 34161791 -
Small
Active Transportation of Liposome Enhances Tumor Accumulation, Penetration, and Therapeutic Efficacy. [Abstract]2020 Nov;16(44):e2004172. PMID: 33030305 -
J Exp Med
EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model. [Abstract]2022 May 2;219(5):e20212414. PMID: 35420633 -
Phytomedicine
Phillygenin improves diabetic nephropathy by inhibiting inflammation and apoptosis via regulating TLR4/MyD88/NF-κB and PI3K/AKT/GSK3β signaling pathways. [Abstract]2025 Jan:136:156314. PMID: 39647467 -
Phytomedicine
β-Caryophyllene promotes the survival of random skin flaps by upregulating the PI3K/AKT signaling pathway. [Abstract]2024 Jul 25:130:155726. PMID: 38815406 -
J Pharm Anal
Impact of FASN-enriched EVs on endothelial cell function in obstructive sleep apnea hypopnea syndrome. [Abstract]2025 May;15(5):101251. PMID: 40521370 -
Mater Today Bio
Membrane biomimetic nanoenzyme-incorporated hybrid glycyrrhizic acid hydrogel for precise mitochondrial ROS scavenging for osteoarthritis treatment. [Abstract]2025 Apr 17:32:101778. PMID: 40290887 -
Acta Pharmacol Sin
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Acta Biomater
A prodrug nanodevice co-delivering docetaxel and ROR1 siRNA for enhanced triple negative breast cancer therapy. [Abstract]2025 Jan 24:193:498-513. PMID: 39730101 -
Cell Death Discov
2024 Jul 19;10(1):329. PMID: 39030174 -
Acta Pharmacol Sin
2022 Apr;43(4):781-787. PMID: 34294887 -
Food Chem
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Mol Psychiatry
CCL5 promotion of bioenergy metabolism is crucial for hippocampal synapse complex and memory formation. [Abstract]2021 Nov;26(11):6451-6468. PMID: 33931731 -
Diabetes
Exofacial Epitope-Specific Antibodies Detect GLUT4 Translocation in Adult Human, Rat, and Mouse Skeletal Muscle. [Abstract]2026 Feb 13:db250567. PMID: 41686119 -
Cell Chem Biol
A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway. [Abstract]2026 Apr 16;33(4):523-537.e7. PMID: 41932333 -
Cell Chem Biol
Identifying enhancers of innate immune signaling as broad-spectrum antivirals active against emerging viruses. [Abstract]2022 Jul 21;29(7):1113-1125.e6. PMID: 35728599 -
Int J Biol Macromol
Porcine TLR8 signaling and its anti-infection function are disturbed by immune checkpoint receptor TIM-3 via inhibition of P13K-AKT pathway. [Abstract]2024 May 1;269(Pt 1):132018. PMID: 38702002 -
New Phytol
Basally distributed actin array drives embryonic hypocotyl elongation during the seed-to-seedling transition in Arabidopsis. [Abstract]2023 Oct;240(1):191-206. PMID: 37537721 -
Microbiol Res
Christensenellaceae minuta modulates epithelial healing via PI3K-AKT pathway and macrophage differentiation in the colitis. [Abstract]2024 Dec:289:127927. PMID: 39393129 -
J Anim Sci Biotechnol
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Clin Transl Med
Tumour cells are sensitised to ferroptosis via RB1CC1-mediated transcriptional reprogramming. [Abstract]2022 Feb;12(2):e747. PMID: 35220675 -
ACS Appl Mater Interfaces
Fullerol Initiates Stem Cell-Nanomaterials Interactions for Enhanced Tissue Regeneration via Clathrin-Mediated Endocytosis and Nuclear Factor Erythroid 2-Related Factor 2 Signaling. [Abstract]2025 Apr 17. PMID: 40241445 -
Br J Cancer
Transcriptome analysis of newly established carboplatin-resistant ovarian cancer cell model reveals genes shared by drug resistance and drug-induced EMT. [Abstract]2023 Mar;128(7):1344-1359. PMID: 36717670 -
Cell Rep
ER-PM tether Syt1 limits cell-to-cell connectivity via plasmodesmata during innate immune responses in Arabidopsis. [Abstract]2025 May 3;44(5):115672. PMID: 40319474 -
Cell Prolif
MCL restrained ROS/AKT/ASAH1 pathway to therapy tamoxifen resistance breast cancer by stabilizing NRF2. [Abstract]2024 Nov;57(11):e13700. PMID: 38924190 -
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Anal Chem
Chemoenzymatic Labeling of Extracellular Vesicles for Visualizing Their Cellular Internalization in Real Time. [Abstract]2020 Jan 21;92(2):2103-2111. PMID: 31876137 -
Cancer Cell Int
PCK1 activates oncogenic autophagy via down-regulation Serine phosphorylation of UBAP2L and antagonizes colorectal cancer growth. [Abstract]2023 Apr 16;23(1):68. PMID: 37062825
Wortmannin purchased from MedChemExpress. Usage Cited in: Cancer Cell Int. 2023 Apr 16;23(1):68. [Abstract]
Wortmannin (1 mM; 24 h) significantly reverses the PCK1-OE-induced increased expression of LC3B II in RKO and SW480 cells.
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Front Immunol
Fpr2/CXCL1/2 Controls Rapid Neutrophil Infiltration to Inhibit Streptococcus agalactiae Infection. [Abstract]2021 Nov 24;12:786602. PMID: 34899755 -
NPJ Regen Med
Cyclic pulsation stress promotes bone formation of tissue engineered laminae through the F-actin/YAP-1/β-Catenin signaling axis. [Abstract]2021 Sep 6;6(1):51. PMID: 34489466 -
Antioxid Redox Signal
SESN2-mediated AKT/GSK-3β/NRF2 Activation to Ameliorate Adriamycin Cardiotoxicity in High Fat Diet-induced Obese Mice. [Abstract]2024 Apr;40(10-12):598-615. PMID: 37265150 -
Ecotoxicol Environ Saf
Cigarette and e-cigarette reversely regulated autophagy via distinct level of ROS in vitro. [Abstract]2025 Jan 1:289:117448. PMID: 39626481 -
Biochem Pharmacol
Almonertinib inhibits liver cancer progression by triggering autophagy-dependent ferroptosis through inhibition of the PI3K/Akt1/mTOR pathway. [Abstract]2026 Mar:245:117628. PMID: 41380804 -
Cell Mol Life Sci
Proximal tubular FHL2, a novel downstream target of hypoxia inducible factor 1, is a protector against ischemic acute kidney injury. [Abstract]2024 May 30;81(1):244. PMID: 38814462 -
Cell Mol Life Sci
HBeAg induces the expression of macrophage miR-155 to accelerate liver injury via promoting production of inflammatory cytokines. [Abstract]2018 Jul;75(14):2627-2641. PMID: 29349567
Wortmannin purchased from MedChemExpress. Usage Cited in: Cell Mol Life Sci. 2018 Jul;75(14):2627-2641. [Abstract]
RAW264.7 macrophages are pre-treated with the inhibitor of ERK, JNK, P38, P65, and AKT signal pathway.Western blot analyzes the non- and phosphorylation of ERK, JNK, P38, P65, and AKT.
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Precis Clin Med
Agrimol B inhibits pancreatic ductal adenocarcinoma by induction of lethal mitophagy through decreasing mitochondrial transcription termination factor 3. [Abstract]2026 Mar 14;9(2):pbag009. PMID: 41978696 -
Life Sci
Nuclear trafficking of PDK1 in importin7-dependent manner is required for insulin-induced AKT ubiquitination. [Abstract]2025 Nov 1:380:123934. PMID: 40921246 -
Life Sci
Taxifolin improves disorders of glucose metabolism and water-salt metabolism in kidney via PI3K/AKT signaling pathway in metabolic syndrome rats. [Abstract]2020 Dec 15:263:118713. PMID: 33157091 -
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EMBO Rep
CaMKII suppresses proteotoxicity by phosphorylating BAG3 in response to proteasomal dysfunction. [Abstract]2024 Oct;25(10):4488-4514. PMID: 39261742 -
Nutrients
Resveratrol and Its Derivatives Diminish Lipid Accumulation in Adipocytes In Vitro-Mechanism of Action and Structure-Activity Relationship. [Abstract]2024 Nov 13;16(22):3869. PMID: 39599655 -
Cell Biol Toxicol
Targeting p38γ synergistically enhances sorafenib-induced cytotoxicity in hepatocellular carcinoma. [Abstract]2025 Jan 28;41(1):35. PMID: 39871031 -
Mar Drugs
Cholestane-3β,5α,6β-triol Induces Multiple Cell Death in A549 Cells via ER Stress and Autophagy Activation. [Abstract]2024 Apr 13;22(4):174. PMID: 38667791 -
Int J Mol Sci
Molecular Mechanisms of Aminoglycoside-Induced Ototoxicity in Murine Auditory Cells: Implications for Otoprotective Drug Development. [Abstract]2025 Jul 13;26(14):6720. PMID: 40724969 -
Int J Mol Sci
D-M159 Synergistically Induces Apoptosis in HeLa Cells Through Endoplasmic Reticulum Stress and Mitochondrial Dysfunction. [Abstract]2025 Mar 29;26(7):3172. PMID: 40243937 -
Int Immunopharmacol
Exploring the molecular mechanism of berberine for treating diabetic nephropathy based on network pharmacology. [Abstract]2024 Jan 5:126:111237. PMID: 37977063 -
Int Immunopharmacol
TRAIP serves as a potential prognostic biomarker and correlates with immune infiltrates in lung adenocarcinoma. [Abstract]2023 Sep:122:110605. PMID: 37451021 -
Int Immunopharmacol
Gitogenin suppresses lung cancer progression by inducing apoptosis and autophagy initiation through the activation of AMPK signaling. [Abstract]2022 Oct:111:108806. PMID: 35914447 -
Glia
Vascular endothelial growth factor promotes transdifferentiation of astrocytes into neurons via activation of the MAPK/Erk-Pax6 signal pathway. [Abstract]2023 Jul;71(7):1648-1666. PMID: 36960578 -
Arthritis Res Ther
Soluble uric acid increases PDZK1 and ABCG2 expression in human intestinal cell lines via the TLR4-NLRP3 inflammasome and PI3K/Akt signaling pathway. [Abstract]2018 Feb 7;20(1):20. PMID: 29415757
Wortmannin purchased from MedChemExpress. Usage Cited in: Arthritis Res Ther. 2018 Feb 7;20(1):20. [Abstract]
Cells preincubated with or without the NF-κB inhibitor PDTC (100 μM), the PI3K inhibitor Wortmannin (3 μg/mL), or DMSO-Control for 2 h. Total protein expression of ABCG2, PDZK1, Akt, p-Akt, and NF-κB p65 determined by western blot analysis. Protein expression normalized to that of GAPDH.
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Molecules
Anemoside B4 Exerts Hypoglycemic Effect by Regulating the Expression of GLUT4 in HFD/STZ Rats. [Abstract]2023 Jan 18;28(3):968. PMID: 36770636 -
Molecules
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Mol Med Rep
Polyphyllin VII induces apoptotic cell death via inhibition of the PI3K/Akt and NF‑κB pathways in A549 human lung cancer cells. [Abstract]2020 Feb;21(2):597-606. PMID: 31974591 -
J Mol Med (Berl)
2019 Aug;97(8):1183-1193. PMID: 31201471 -
Sci Rep
NDRG4 overexpression is associated with reduced apoptosis after intracerebral hemorrhage via the PI3K/Akt/GSK3β signaling pathway. [Abstract]2026 Jan 3;16(1):3374. PMID: 41484166 -
Cancer Sci
LPAR5 promotes thyroid carcinoma cell proliferation and migration by activating class IA PI3K catalytic subunit p110β. [Abstract]2021 Apr;112(4):1624-1632. PMID: 33540491 -
PLoS Pathog
BRD4 inhibition exerts anti-viral activity through DNA damage-dependent innate immune responses. [Abstract]2020 Mar 24;16(3):e1008429. PMID: 32208449 -
Cell Signal
Paeoniflorin-6'-O-benzene sulfonate inhibits keratinocyte proliferation by restoring GRK2-JAK1 colocalization in mouse model of psoriasis. [Abstract]2025 Jul:131:111706. PMID: 40037425 -
J Cell Mol Med
Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis. [Abstract]2021 Jun;25(11):5238-5249. PMID: 33951300 -
Oncol Rep
Matrine improves skeletal muscle atrophy by inhibiting E3 ubiquitin ligases and activating the Akt/mTOR/FoxO3α signaling pathway in C2C12 myotubes and mice. [Abstract]2019 Aug;42(2):479-494. PMID: 31233199 -
Plant Sci
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Front Mol Biosci
NPC1 Deficiency Contributes to Autophagy-Dependent Ferritinophagy in HEI-OC1 Auditory Cells. [Abstract]2022 Jul 22:9:952608. PMID: 35936782 -
FASEB J
AKT2/mTOR/VEGF Signaling Attenuates Aging-Related Male Infertility by Suppressing Epididymal Autophagy and Apoptosis via Extracellular Vesicle-Mediated Sperm Rescue. [Abstract]2025 Aug 31;39(16):e70901. PMID: 40810376 -
FASEB J
AGDMP1 alleviates insulin resistance by modulating heat shock protein 60-mediated IRS-1/AKT/GLUT4 pathway and adipose inflammation: A potential therapeutic peptide for gestational diabetes mellitus. [Abstract]2025 Jan 31;39(2):e70339. PMID: 39854096 -
FASEB J
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Front Physiol
High Glucose Attenuates Cardioprotective Effects of Glucagon-Like Peptide-1 Through Induction of Mitochondria Dysfunction via Inhibition of β-Arrestin-Signaling. [Abstract]2021 May 13:12:648399. PMID: 34054568 -
Fish Shellfish Immunol
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J Virol
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J Virol
2025 Aug 11:e0077125. PMID: 40788012 -
J Virol
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J Virol
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Chem Res Toxicol
Cardiac Glycoside Compound Isolated from Helleborus thibetanus Franch Displays Potent Toxicity against HeLa Cervical Carcinoma Cells through ROS-Independent Autophagy. [Abstract]2019 Dec 16;32(12):2479-2487. PMID: 31714069 -
Naunyn Schmiedebergs Arch Pharmacol
Aucubin inhibits epithelial-mesenchymal transition and angiogenesis in colorectal cancer via the HDAC6/PI3K/Akt signaling pathway. [Abstract]2026 Apr 18. PMID: 41999432 -
J Cell Physiol
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Virol J
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Viruses
The Main Protease of Middle East Respiratory Syndrome Coronavirus Induces Cleavage of Mitochondrial Antiviral Signaling Protein to Antagonize the Innate Immune Response. [Abstract]2024 Feb 5;16(2):256. PMID: 38400032 -
Viruses
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Mol Immunol
Inhibition of histone deacetylase 1 suppresses pseudorabies virus infection through cGAS-STING antiviral innate immunity. [Abstract]2021 Aug:136:55-64. PMID: 34087624 -
Int J Med Sci
Terlipressin relieves intestinal and renal injuries induced by acute mesenteric ischemia via PI3K/Akt pathway. [Abstract]2020 Sep 28;17(17):2751-2762. PMID: 33162803 -
Toxicol Appl Pharmacol
The skin photoprotective effect of trilinolein: Induction of cellular autophagy via the AMPK-mTOR signaling pathway. [Abstract]2024 Feb:483:116836. PMID: 38272316 -
PLoS Negl Trop Dis
Sporothrix globosa melanin regulates autophagy via the TLR2 signaling pathway in THP-1 macrophages. [Abstract]2023 May 4;17(5):e0011281. PMID: 37141335 -
Cardiovasc Drugs Ther
Midkine Promote Atherosclerosis by Regulating the Expression of ATP-Binding Cassette Transporter A1 via Activator Protein-1. [Abstract]2025 May 22. PMID: 40402391 -
Arch Biochem Biophys
Handelin protects human skin keratinocytes against ultraviolet B-induced photodamage via autophagy activation by regulating the AMPK-mTOR signaling pathway. [Abstract]2023 Jul 15:743:109646. PMID: 37225010 -
J Reprod Immunol
Chloroquine is a safe autophagy inhibitor for sustaining the expression of antioxidant enzymes in trophoblasts. [Abstract]2023 Feb:155:103766. PMID: 36470134 -
J Cosmet Dermatol
2022 Mar;21(3):1163-1170. PMID: 33894036 -
Arch Biochem Biophys
2021 Oct 30:711:109015. PMID: 34437865 -
Diabetes Metab Syndr Obes
Essential Role Of High Glucose-Induced Overexpression Of PKCβ And PKCδ In GLP-1 Resistance In Rodent Cardiomyocytes. [Abstract]2019 Nov 4:12:2289-2302. PMID: 31807042 -
J Leukoc Biol
2026 Jun 27:qiag090. PMID: 42364103 -
Brain Sci
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Food Chem Toxicol
α-Humulene inhibits hepatocellular carcinoma cell proliferation and induces apoptosis through the inhibition of Akt signaling. [Abstract]2019 Dec:134:110830. PMID: 31562948 -
Brain Res
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Polymers
Chitosan Oligosaccharide Promotes Junction Barrier through Modulation of PI3K/AKT and ERK Signaling Intricate Interplay in T84 Cells. [Abstract]2023 Mar 28;15(7):1681. PMID: 37050295
Wortmannin purchased from MedChemExpress. Usage Cited in: Polymers. 2023 Mar 28;15(7):1681. [Abstract]
Wortmannin (5.0, 10 μM; 24 h) significantly induces cell death in T84 cells.
Wortmannin purchased from MedChemExpress. Usage Cited in: Polymers. 2023 Mar 28;15(7):1681. [Abstract]
Wortmannin (2 µM; 2, 4, 6 h) decreases the expression of p-AKT and the ratio of p-AKT/AKT while increases the expression of p-ERK and the ratio of p-ERK/ERK in T84 cells.
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Vet Microbiol
The important role of TLR2/MyD88/JNK in regulating the pathogenesis and inflammation induced by pseudorabies virus in mice. [Abstract]2025 May:304:110496. PMID: 40156971 -
Vet Microbiol
Epidermal growth factor receptor (EGFR) promotes uptake of bovine parainfluenza virus type 3 into MDBK cells. [Abstract]2022 Aug:271:109488. PMID: 35691094 -
Vet Parasitol
2026 May:344:110769. PMID: 41967379 -
Virology
BCX4430 inhibits the replication of rabies virus by suppressing mTOR-dependent autophagy invitro. [Abstract]2023 Aug:585:21-31. PMID: 37267717 -
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Clin Cosmet Investig Dermatol
Salidroside Ameliorates Ultraviolet-Induced Keratinocyte Injury by Inducing SIRT1-Dependent Autophagy. [Abstract]2022 Aug 2;15:1499-1508. PMID: 35941856 -
J Cardiovasc Pharmacol
Carbon Monoxide-Saturated Polymerized Placenta Hemoglobin Optimizes Mitochondrial Function and Protects Heart Against Ischemia-Reperfusion Injury. [Abstract]2021 Jun 1;77(6):814-821. PMID: 34001725 -
Clin Exp Pharmacol Physiol
ELABELA improves endothelial cell function via the ELA-APJ axis by activating the PI3K/Akt signalling pathway in HUVECs and EA.hy926 cells. [Abstract]2020 Dec;47(12):1953-1964. PMID: 32687618 -
Biochem Biophys Res Commun
MEK and PI3K catalytic activity as predictor of the response to molecularly targeted agents in triple-negative breast cancer. [Abstract]2017 Aug 5;489(4):484-489. PMID: 28576487 -
Int J Ophthalmol
Autophagy serves as a protective effect against inflammatory injury of oxidative stress in ARPE-19 cell. [Abstract]2025 Jan 18;18(1):28-38. PMID: 39829617 -
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Biosci Biotechnol Biochem
Kangfuxin liquid reduces the ultraviolet B-induced photodamage of HaCaT cells by regulating autophagy. [Abstract]2023 Nov 21;87(12):1485-1494. PMID: 37682519 -
J Vis Exp
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Stem Cell Res
PI3K-Akt signaling regulates BMP2-induced osteogenic differentiation of mesenchymal stem cells (MSCs): A transcriptomic landscape analysis. [Abstract]2023 Feb:66:103010. PMID: 36580886 -
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bioRxiv
2026 May 19:2026.05.17.725791. PMID: 42239437 -
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Heliyon
Overexpression of mir-489-3p inhibits proliferation and migration of non-small cell lung cancer cells by suppressing the HER2/PI3K/AKT/Snail signaling pathway. [Abstract]2024 Aug 6;10(16):e35832. PMID: 39224367 -
Environ Toxicol
Asiatic acid inhibits osteosarcoma cell migration and invasion via the AKT/Sp1/MMP1 axis. [Abstract]2024 Jul;39(7):3920-3929. PMID: 38567545 -
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Elife
2024 Feb 20:13:e85985. PMID: 38375778 -
Environ Toxicol
2023 Nov;38(11):2621-2631. PMID: 37466199 -
Wortmannin purchased from MedChemExpress. Usage Cited in: bioRxiv. 2023 Feb 14.
Both of LY294002 (LY; 30 μM) and Wortmannin (Wort; 50 nM) can downregulate the expression of ACE2 in HPAEpiC cells.
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Environ Toxicol
PI3K/AKT/mTOR pathway mediated-cell cycle dysregulation contribute to malignant proliferation of mouse spermatogonia induced by microcystin-leucine arginine. [Abstract]2023 Feb;38(2):343-358. PMID: 36288207 -
Front Med
Cannabidiol prevents depressive-like behaviors through the modulation of neural stem cell differentiation. [Abstract]2022 Apr;16(2):227-239. PMID: 35212887 -
Ann Transl Med
Downregulated placental expression of linc00468 contributes to trophoblast dysfunction by inducing epithelial-mesenchymal transition. [Abstract]2020 Mar;8(6):333. PMID: 32355777 -
Ann Transl Med
Left ventricular response in the transition from hypertrophy to failure recapitulates distinct roles of Akt, β-arrestin-2, and CaMKII in mice with aortic regurgitation. [Abstract]2020 Mar;8(5):219. PMID: 32309366
Solvente y solubilidad
In Vitro:
DMSO : ≥ 50 mg/mL (116.71 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: 2.08 mg/mL (4.85 mM); Suspended solution; Need ultrasonic
This protocol yields a suspended solution of 2.08 mg/mL. Suspended solution can be used for oral and intraperitoneal injection.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.85 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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-
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
-
%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
-
%+
-
+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (292 KB)
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SDS (481 KB)
- English - EN (481 KB)
- Français - FR (481 KB)
- Deutsch - DE (481 KB)
- Norwegian - NO (481 KB)
- Español - ES (481 KB)
- Swedish - SV (481 KB)
- Italian - IT (481 KB)
- Korean - KR (481 KB)
- Portuguese - PT (481 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Yano H, et al. Inhibition of histamine secretion by wortmannin through the blockade of phosphatidylinositol 3-kinase in RBL-2H3 cells. J Biol Chem. 1993 Dec 5;268(34):25846-56. [Content Brief]
[2]. Moon EK, et al. Autophagy inhibitors as a potential antiamoebic treatment for Acanthamoeba keratitis. Antimicrob Agents Chemother. 2015 Jul;59(7):4020-5. [Content Brief]
[3]. Liu Y, et al. Polo-like kinases inhibited by wortmannin. Labeling site and downstream effects. J Biol Chem. 2007 Jan 26;282(4):2505-11. [Content Brief]
[4]. Wu Q, et al. Wortmannin inhibits K562 leukemic cells by regulating PI3k/Akt channel in vitro. J Huazhong Univ Sci Technolog Med Sci. 2009 Aug;29(4):451-6. [Content Brief]
[5]. Lemke LE, et al. Wortmannin inhibits the growth of mammary tumors despite the existence of a novel wortmannin-insensitive phosphatidylinositol-3-kinase. Cancer Chemother Pharmacol. 1999;44(6):491-7. [Content Brief]
[6]. Liu Y, et al. Wortmannin, a widely used phosphoinositide 3-kinase inhibitor, also potently inhibits mammalianpolo-like kinase. Chem Biol. 2005 Jan;12(1):99-107. [Content Brief]
[7]. Pobbati AV, et al. A combat with the YAP/TAZ-TEAD oncoproteins for cancer therapy. Theranostics. 2020 Feb 18;10(8):3622-3635. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.3341 mL | 11.6705 mL | 23.3410 mL | 58.3526 mL |
| 5 mM | 0.4668 mL | 2.3341 mL | 4.6682 mL | 11.6705 mL | |
| 10 mM | 0.2334 mL | 1.1671 mL | 2.3341 mL | 5.8353 mL | |
| 15 mM | 0.1556 mL | 0.7780 mL | 1.5561 mL | 3.8902 mL | |
| 20 mM | 0.1167 mL | 0.5835 mL | 1.1671 mL | 2.9176 mL | |
| 25 mM | 0.0934 mL | 0.4668 mL | 0.9336 mL | 2.3341 mL | |
| 30 mM | 0.0778 mL | 0.3890 mL | 0.7780 mL | 1.9451 mL | |
| 40 mM | 0.0584 mL | 0.2918 mL | 0.5835 mL | 1.4588 mL | |
| 50 mM | 0.0467 mL | 0.2334 mL | 0.4668 mL | 1.1671 mL | |
| 60 mM | 0.0389 mL | 0.1945 mL | 0.3890 mL | 0.9725 mL | |
| 80 mM | 0.0292 mL | 0.1459 mL | 0.2918 mL | 0.7294 mL | |
| 100 mM | 0.0233 mL | 0.1167 mL | 0.2334 mL | 0.5835 mL |
Wortmannin Related Classifications
HY-10197 Related Classifications
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