Go 6983 

Go 6983 GMP is Go 6983 (HY-13689) produced by using GMP guidelines. GMP small molecules works appropriately as an auxiliary reagent for cell therapy manufacture. Go 6983 is a dual inhibitor targeting Suv39h1/2 (KMT1A/KMT1B) and PKC, as well as a transcriptional activator capable of inducing DNA hypomethylation. Go 6983 stimulates the transcription of Prdm14 by reducing Suv39h1/2 protein levels, decreasing histone modifications in the Prdm14 promoter region, and increasing the recruitment of RNA polymerase II. Go 6983 induces genome-wide DNA hypomethylation by inhibiting de novo methyltransferase expression and increasing Tet1/Tet2 levels, thereby promoting self-renewal and pluripotency maintenance of stem cells. Meanwhile, Go 6983 can block PKC-mediated signaling pathways to reduce the expression of EMT-related genes and eliminate the upregulation of antioxidant genes downstream of NRF2. Go 6983 is mainly used in mechanism studies related to myocardial ischemia/reperfusion injury^[1][2][3].

Go 6983 GMP (5 μM; 8 d) promotes short-term self-renewal and maintains pluripotency of 46C mouse embryonic stem cells in both serum-containing medium without LIF and serum-free N2B27 medium^[1].
Go 6983 GMP (5 μM; 8 d) promotes the self-renewal of 46C mouse embryonic stem cells via Prdm14, as overexpression of Prdm14 mimics the activity of Go6983, while knockdown of Prdm14 abolishes Go6983-mediated pluripotency maintenance^[1].
Go 6983 GMP (5 μM; 24 h) promotes short-term self-renewal of human induced pluripotent stem cells, and this effect is mediated by Prdm14 through inhibiting the expression of Dnmt3 gene^[1].
Go 6983 GMP (5 μM; 12 h, 24 h) enhances the expression of Prdm14 in 46C mouse embryonic stem cells by reducing the expression levels of Suv39h1 and Suv39h2^[1].
Go 6983 (5 μM) blocks Dihydrotanshinone I (HY-N0360)-induced NRF2 nuclear accumulation by inhibiting nuclear import, and this process cannot be reversed by the nuclear export inhibitory effect of leptomycin B in HL-1 mouse cardiomyocytes^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

442.51

C₂₆H₂₆N₄O₃

133053-19-7

O=C(C(C1=CN(CCCN(C)C)C2=C1C=C(OC)C=C2)=C3C4=CNC5=C4C=CC=C5)NC3=O

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Ji J, et al. Inhibition of protein kinase C increases Prdm14 level to promote self-renewal of embryonic stem cells through reducing Suv39h-induced H3K9 methylation. J Biol Chem. 2024;300(3):105714.  [Content Brief]

  [2]. Zeng H, et al. Activated PKB/GSK-3β synergizes with PKC-δ signaling in attenuating myocardial ischemia/reperfusion injury via potentiation of NRF2 activity: Therapeutic efficacy of dihydrotanshinone-I. Acta Pharm Sin B. 2021;11(1):71-88.  [Content Brief]

  [3]. Yu CY, et al. The Trans-Spliced Long Noncoding RNA tsRMST Impedes Human Embryonic Stem Cell Differentiation Through WNT5A-Mediated Inhibition of the Epithelial-to-Mesenchymal Transition. Stem Cells. 2016;34(8):2052-2062.