2. NF-κB MAPK/ERK Pathway PI3K/Akt/mTOR GPCR/G Protein Apoptosis Immunology/Inflammation
  3. NF-κB p38 MAPK Akt Prostaglandin Receptor TNF Receptor Interleukin Related NO Synthase COX
  4. Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside 

Cat. No.: HY-N18025
Handling Instructions Technical Support

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside is an anti-inflammatory agent. Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside exerts significant anti-inflammatory effects in LPS (HY-D1056)-induced RAW 264.7 macrophages by inhibiting the NF-κB, MAPK and Akt signaling pathways, thereby reducing the production of inflammatory mediators (NO, PGE2) and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6).

For research use only. We do not sell to patients.

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside Chemical Structure

CAS No. : 131559-50-7

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Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside Related Antibodies

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NF-κB NF-κB1/p50 RelA/p65 RelB c-Rel

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nNOS iNOS eNOS

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  • Biological Activity

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Description

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside is an anti-inflammatory agent. Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside exerts significant anti-inflammatory effects in LPS (HY-D1056)-induced RAW 264.7 macrophages by inhibiting the NF-κB, MAPK and Akt signaling pathways, thereby reducing the production of inflammatory mediators (NO, PGE2) and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6)[1].

IC50 & Target[1]

COX-2

 

iNOS

 

IL-6

 

IL-1β

 

In Vitro

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-40 μM; 1 h pre-incubation prior to 24 h LPS stimulation) concentration-dependently inhibits LPS-induced NO production in RAW 264.7 cells[1].
Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-5 μM; 1 h pre-incubation prior to 24 h LPS stimulation) concentration-dependently inhibits LPS-induced production of PGE2, TNF-α, IL-1β, and IL-6 in RAW 264.7 cells[1].
Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-5 μM; 1 h pre-incubation prior to LPS stimulation) concentration-dependently inhibits LPS-induced activation of the NF-κB pathway in RAW 264.7 cells by reducing phosphorylation of NF-κB p65 and IκB and restoring total IκB expression[1].
Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-5 μM) concentration-dependently inhibits LPS-induced expression of iNOS and COX-2 proteins in RAW 264.7 cells[1].
Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-5 μM) concentration-dependently inhibits LPS-induced activation of the MAPKs pathway in RAW 264.7 cells by reducing phosphorylation of p38, JNK, and Erk[1].
Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside (1.25-5 μM) concentration-dependently inhibits LPS-induced activation of the Akt pathway in RAW 264.7 cells by reducing phosphorylation of Akt[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside Related Antibodies

Cell Viability Assay[1]

Cell Line: RAW 264.7
Concentration: 1.25, 2.5, 5, 10, 20 and 40 μM
Incubation Time: 24 h
Result: Showed no significant effect on cell viability.

ELISA Assay[1]

Cell Line: RAW 264.7 (LPS-stimulated)
Concentration: 1.25, 2.5 and 5 μM
Incubation Time: 1 h pre-incubation; 24 h LPS stimulation
Result: Concentration-dependently reduced LPS-induced production of PGE2, TNF-α, IL-1β, and IL-6, with significant inhibition at all tested concentrations.

Western Blot Analysis[1]

Cell Line: RAW 264.7 (LPS-stimulated)
Concentration: 1.25, 2.5 and 5 μM
Incubation Time: 1 h pre-incubation; LPS stimulation
Result: Concentration-dependently reduced LPS-induced phosphorylation of NF-κB p65 and IκB.
Concentration-dependently enhanced LPS-reduced total IκB protein expression.
Left total NF-κB p65 levels unaffected.
Molecular Weight

918.80

Formula

C39H50O25
CAS No.
SMILES

O[C@H]([C@@H](CO[C@@H]1O[C@@H](C)[C@H](O)[C@@H](O)[C@H]1O)O2)[C@H](O)[C@@H](O[C@H]3[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O3)[C@@H]2OC4=C(C5=CC=C(O)C=C5)OC6=CC(O[C@H]7[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O7)=CC(O)=C6C4=O
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside Related Classifications

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    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Kaempferol 3-O-(2G-glucosylrutinoside)-7-O-glucoside131559-50-7NF-κBp38 MAPKAktProstaglandin ReceptorTNF ReceptorInterleukin RelatedNO SynthaseCOXNuclear factor-κBNuclear factor-kappaBPKBProtein kinase BTumor Necrosis Factor ReceptorTNFRILNitric oxide synthasesNOSCyclooxygenaseIκBNF-κB p65JNKErkcyclooxygenase 2inducible nitric oxide synthaseCOX-2Hosta plantagineaInhibitorinhibitorinhibit

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