RGD peptide (GRGDNP)

Name: RGD peptide (GRGDNP)
Brand: MedChemExpress
SKU: HY-P1740
Rating: 5.0 (7 reviews)

Cat. No.: HY-P1740 Purity: 99.83%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

RGD peptide (GRGDNP) is an inhibitor of integrin-ligand interactions. RGD peptide (GRGDNP) competitively inhibits α5β1 binding with extracellular matrice (ECM). RGD peptide (GRGDNP) promotes apoptosis through activation of conformation changes that enhance pro-caspase-3 activation and autoprocessing. RGD peptide (GRGDNP) plays an important role in cell adhesion, migration, growth, and differentiation.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

CAS No. : 114681-65-1

Size	Stock	Quantity
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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Customer Review

Based on 7 publication(s) in Google Scholar

Other Forms of RGD peptide (GRGDNP):

RGD peptide (GRGDNP) TFA In-stock

7 Publications Citing Use of MCE RGD peptide (GRGDNP)

•bioRxiv. 2024 October 06.

Bio/Physico-chemical Assay

: RGD peptide (GRGDNP) purchased from MedChemExpress. Usage Cited in: Adv Mater. 2024 Jul 18:e2406140. [Abstract]; Comparison of the adhesion of B-GEM and R-GEM cells to 4T1 tumor cells after 10 min of coincubation in medium preadded with free RGD peptide (0-8 μM). Exogenous free RGD dose-dependently reversed the increase in adhesion of R-GEM cells to tumor cells.

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•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Integrin Isoform Specific Products:

View All Isoforms

αvβ1 αvβ3 αvβ5 αvβ6 αvβ8 α5β1 α1β1 α2β1 α4β1 α9β1 αIIbβ3 α4β7 αLβ2

View All Caspase Isoform Specific Products:

View All Isoforms

Caspase 1 Caspase 2 Caspase 3 Caspase 4 Caspase 5 Caspase 6 Caspase 7 Caspase 8 Caspase 9 Caspase 10 Caspase 12 Caspase Caspase 11 Caspase-13

Biological Activity
Purity & Documentation
References
Customer Review

Description

IC₅₀ & Target^[2]

α5β1

Caspase 3

Cellular Effect

Cell Line	Type	Value	Description	References
K562	IC₅₀	> 100 μM Compound: H-Gly-Arg-Gly-Asp-Asn-Pro-OH	Inhibition of alpha5beta1 integrin-mediated human K562 cell adhesion to fibronectin incubated for 30 mins before plating measured after 1 hr Inhibition of alpha5beta1 integrin-mediated human K562 cell adhesion to fibronectin incubated for 30 mins before plating measured after 1 hr	[PMID: 23811088]

In Vitro

RGD peptide (GRGDNP) (50 μM ; preincubation for 3 hours before stretch) abolishes stretch-induced IKK activation and IL-6 mRNA expression. It shows little effect on the IKK activity and IL-6 mRNA expression in unstretched HUVECs^[2].
RGD peptide (GRGDNP) (300 μg/mL; for 6 hours) completely reverses enhanced FN1 expression in oxygen glucose deprivation(OGD) treated primary hippocampal neurons, HT22 cell lines and in its sEVs^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

614.61

Formula

C₂₃H₃₈N₁₀O₁₀

CAS No.

114681-65-1

Appearance

Solid

Color

White to off-white

Sequence

Gly-Arg-Gly-Asp-Asn-Pro

Sequence Shortening

GRGDNP

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Sealed storage, away from moisture

Powder	-80°C	2 years
	-20°C	1 year

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

H₂O : ≥ 100 mg/mL (162.70 mM)

DMSO : 3.12 mg/mL (5.08 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	1.6270 mL	8.1352 mL	16.2705 mL
5 mM	0.3254 mL	1.6270 mL	3.2541 mL
10 mM	0.1627 mL	0.8135 mL	1.6270 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 0.67 mg/mL (1.09 mM); Clear solution

This protocol yields a clear solution of ≥ 0.67 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (6.7 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Purity & Documentation

Purity: 99.83%

Select Batch:

Data Sheet (278 KB) SDS (393 KB)

COA (248 KB) RP-HPLC (207 KB)

Handling Instructions (2659 KB)

References

[1]. C D Buckley, et al. RGD peptides induce apoptosis by direct caspase-3 activation. Nature. 1999 Feb 11;397(6719):534-9. [Content Brief]

[2]. Wei Xia, et al. Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors. Nat Commun. 2021 Oct 15;12(1):6043. [Content Brief]

[3]. Akitoshi Sasamoto, et al. Mechanotransduction by integrin is essential for IL-6 secretion from endothelial cells in response to uniaxial continuous stretch. Am J Physiol Cell Physiol. 2005 May;288(5):C1012-22. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO / H₂O	1 mM	1.6270 mL	8.1352 mL	16.2705 mL	40.6762 mL
DMSO / H₂O	5 mM	0.3254 mL	1.6270 mL	3.2541 mL	8.1352 mL
H₂O	10 mM	0.1627 mL	0.8135 mL	1.6270 mL	4.0676 mL
	15 mM	0.1085 mL	0.5423 mL	1.0847 mL	2.7117 mL
	20 mM	0.0814 mL	0.4068 mL	0.8135 mL	2.0338 mL
	25 mM	0.0651 mL	0.3254 mL	0.6508 mL	1.6270 mL
	30 mM	0.0542 mL	0.2712 mL	0.5423 mL	1.3559 mL
	40 mM	0.0407 mL	0.2034 mL	0.4068 mL	1.0169 mL
	50 mM	0.0325 mL	0.1627 mL	0.3254 mL	0.8135 mL
	60 mM	0.0271 mL	0.1356 mL	0.2712 mL	0.6779 mL
	80 mM	0.0203 mL	0.1017 mL	0.2034 mL	0.5085 mL
	100 mM	0.0163 mL	0.0814 mL	0.1627 mL	0.4068 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.